BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0024.p (160 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310895|ref|YP_003774987.1| dipeptide/oligopeptide/nickel A... 139 1e-34 gi|300312716|ref|YP_003776808.1| ABC transporter duplicated ATPa... 129 2e-31 gi|300312478|ref|YP_003776570.1| dipeptide/oligopeptide/nickel A... 126 8e-31 gi|300311168|ref|YP_003775260.1| ABC transporter ATPase [Herbasp... 125 1e-30 gi|300310466|ref|YP_003774558.1| dipeptide/oligopeptide/nickel A... 92 2e-20 >gi|300310895|ref|YP_003774987.1| dipeptide/oligopeptide/nickel ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 139 bits (349), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 68/158 (43%), Positives = 107/158 (67%) Query: 2 ITLRDLQVQFFAPKKLIRAVNGVNLTVGQGEVVALIGESGSGKSVTLRSIMRLHSERSTR 61 + +R+L+ FF P+ ++ AV+ V+L+VG+G ++ L+GESGSGKSVT SI+ + Sbjct: 6 LEVRNLRTHFFTPEGVLPAVDDVSLSVGRGRILGLVGESGSGKSVTGFSILGMVDAPGRI 65 Query: 62 ISGGLEVDGKDVLKMSQRELAALRGDTVAMIFQEPLLALDPVYTVGAQIIEAIRRHKKVS 121 + G + G+D+++M + L L+G+ +AMIFQ+P++ L+PV V AQ+++A+ H KVS Sbjct: 66 VGGEILFQGRDLVRMDKTSLRELQGNRIAMIFQDPMMTLNPVLKVEAQMVDAVLAHSKVS 125 Query: 122 KAEAHRQALSLFQKVRIPSPERRLDAYPHEMSGGMRQR 159 + +A A + I SPE RL AYPH++SGGMRQR Sbjct: 126 RQQARELARDTLGMMGIASPEERLQAYPHQLSGGMRQR 163 >gi|300312716|ref|YP_003776808.1| ABC transporter duplicated ATPase [Herbaspirillum seropedicae SmR1] Length = 529 Score = 129 bits (323), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 65/140 (46%), Positives = 100/140 (71%), Gaps = 2/140 (1%) Query: 21 VNGVNLTVGQGEVVALIGESGSGKSVTLRSIMRLHSERSTRISGGLEVDGKDVLKMSQRE 80 V+ V+ + GE AL+GESGSGK+V+ ++M+L+ + R G + +DG+DVL S+R+ Sbjct: 21 VDRVSFAIAPGEKFALVGESGSGKTVSSLAVMQLNQD--ARYEGSILLDGQDVLAKSERQ 78 Query: 81 LAALRGDTVAMIFQEPLLALDPVYTVGAQIIEAIRRHKKVSKAEAHRQALSLFQKVRIPS 140 + +RG+ VAMIFQEP+ AL+P++T+G QI E + H+ ++ +A R+A++L +K IP Sbjct: 79 MQQVRGNDVAMIFQEPMTALNPLFTIGNQIAEVLMHHEGLNARDAARRAVALLEKTGIPE 138 Query: 141 PERRLDAYPHEMSGGMRQRA 160 P RR +YPHE+SGG RQRA Sbjct: 139 PARRALSYPHELSGGQRQRA 158 Score = 84.3 bits (207), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 55/150 (36%), Positives = 87/150 (58%), Gaps = 8/150 (5%) Query: 11 FFAPKKLIRAVNGVNLTVGQGEVVALIGESGSGKSVTLRSIMRLHSERSTRISGGLEVDG 70 +FA K+ AV+GV+L + +GE + ++GESGSGKS +++RL + ++G + DG Sbjct: 284 WFA-KRAFHAVDGVDLQLRRGETLGIVGESGSGKSTLGMALLRLS---AASVAGSIRFDG 339 Query: 71 KDVLKMSQRELAALRGDTVAMIFQEPLLALDPVYTVGAQIIEAIRRHKKVSKAEAHRQAL 130 + + QREL LR + ++FQ+P +L P TV + E + H AE RQA+ Sbjct: 340 VPISERPQRELRPLR-SRMQVVFQDPFASLSPRRTVEQIVGEGLELHHPDRSAEQQRQAI 398 Query: 131 -SLFQKVRIPSPERRLDAYPHEMSGGMRQR 159 + ++V + + L+ YPHE SGG RQR Sbjct: 399 VAALEEVGLSAA--VLERYPHEFSGGQRQR 426 >gi|300312478|ref|YP_003776570.1| dipeptide/oligopeptide/nickel ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 334 Score = 126 bits (317), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 63/159 (39%), Positives = 102/159 (64%) Query: 1 MITLRDLQVQFFAPKKLIRAVNGVNLTVGQGEVVALIGESGSGKSVTLRSIMRLHSERST 60 ++ +++L+V ++ AV G++ V +GE++ L+GESG GKS+T ++M L ++ Sbjct: 4 LLDVKNLKVDLPTENGMLHAVRGIDFQVRRGEMLCLVGESGCGKSMTSLALMGLLPRKAQ 63 Query: 61 RISGGLEVDGKDVLKMSQRELAALRGDTVAMIFQEPLLALDPVYTVGAQIIEAIRRHKKV 120 + + DG D+ M ++L LRG +AMIFQEP+ +L+P YT+G Q+ EA+ + V Sbjct: 64 CSADHILFDGVDLHGMPDKQLMQLRGKRMAMIFQEPMTSLNPSYTLGNQLCEAMLQQPGV 123 Query: 121 SKAEAHRQALSLFQKVRIPSPERRLDAYPHEMSGGMRQR 159 S+AEA +AL L + I + E RL YPH++SGG+RQR Sbjct: 124 SRAEARERALYLLHRTGISNAEDRLRQYPHQLSGGLRQR 162 >gi|300311168|ref|YP_003775260.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 617 Score = 125 bits (314), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 68/165 (41%), Positives = 106/165 (64%), Gaps = 6/165 (3%) Query: 1 MITLRDLQVQFFAPKKLIRAVNGVNLTVGQGEVVALIGESGSGKSVTLRSIMRLHSERST 60 ++ + L V+F ++ + AV ++ V QGE +A++GESGSGKSV+ +IMRL Sbjct: 7 VLDVNHLSVRFSTSERTVDAVRDLSFHVDQGETLAIVGESGSGKSVSSLAIMRLIEHGGG 66 Query: 61 RISGG----LEVDGK--DVLKMSQRELAALRGDTVAMIFQEPLLALDPVYTVGAQIIEAI 114 +I+ G L G+ D+ + + ++RG +AMIFQEP+ +L+PV+TVG QI E+I Sbjct: 67 KIASGSMQFLRRSGQRLDLAQADNATMRSIRGAEIAMIFQEPMTSLNPVFTVGEQIAESI 126 Query: 115 RRHKKVSKAEAHRQALSLFQKVRIPSPERRLDAYPHEMSGGMRQR 159 R H+ + +A A +AL + + VRIP +R L +PH++SGGMRQR Sbjct: 127 RLHQGLGRAAAQAEALRMLEMVRIPEAKRVLGRHPHQLSGGMRQR 171 Score = 86.7 bits (213), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 62/167 (37%), Positives = 93/167 (55%), Gaps = 18/167 (10%) Query: 1 MITLRDLQVQFFAP-------KKLIRAVNGVNLTVGQGEVVALIGESGSGKSVTLRSIMR 53 ++ +RDL +F ++ + AV V+ + GE +A++GESG GKS T RS++R Sbjct: 312 ILKVRDLTTRFDVKSGIFGRVRQRVHAVEKVSFDLYAGETLAIVGESGCGKSTTGRSLLR 371 Query: 54 LHSERSTRISGG-LEVDGKDVLKMSQRELAALRGDTVAMIFQEPLLALDPVYTVGAQIIE 112 L I+GG +E G+D+ ++ + EL LR D + IFQ+P +LDP TVG I+E Sbjct: 372 L-----VDIAGGKVEFGGRDLAQLPRSELRQLRRD-IQFIFQDPFASLDPRMTVGYSIME 425 Query: 113 AIRRHKKVSKAEAHRQALSLFQKVRIPSPERRLDAYPHEMSGGMRQR 159 + H K A + +L +V + +PE YPHE SGG RQR Sbjct: 426 PMLVHGM--KEGAQERVDALLTRVGL-TPEHG-QRYPHEFSGGQRQR 468 >gi|300310466|ref|YP_003774558.1| dipeptide/oligopeptide/nickel ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 645 Score = 91.7 bits (226), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 55/143 (38%), Positives = 89/143 (62%), Gaps = 2/143 (1%) Query: 18 IRAVNGVNLTVGQGEVVALIGESGSGKSVTLRSIMRLHSERSTRISGG-LEVDGKDVLKM 76 + V VN TV +GEV+ALIGESGSGK+ S++ ++ I+GG + + + + +M Sbjct: 46 VEIVKNVNFTVARGEVLALIGESGSGKTTIALSLLG-YARAGCHIAGGQVRIGERQIDQM 104 Query: 77 SQRELAALRGDTVAMIFQEPLLALDPVYTVGAQIIEAIRRHKKVSKAEAHRQALSLFQKV 136 ++ L A+RG VA I Q A +P T+ Q+IE+ +H + KA A R+A+ LF+ + Sbjct: 105 DEKALLAMRGHHVAYIAQSAAAAFNPARTLMDQVIESALQHGVMDKASAMRKAVELFRAL 164 Query: 137 RIPSPERRLDAYPHEMSGGMRQR 159 +P+P++ + YPH++SGG QR Sbjct: 165 ALPNPDKIGERYPHQVSGGQLQR 187 Score = 57.4 bits (137), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 43/156 (27%), Positives = 81/156 (51%), Gaps = 17/156 (10%) Query: 11 FFAPKKL----IRAVNGVNLTVGQGEVVALIGESGSGKSVTLRSIMRLHSERSTRISGGL 66 + AP +L +R ++ +NLT+ +G V +IGESGSGK+ R++ + + G + Sbjct: 318 YGAPDRLGLPGVRILDDINLTIQRGSTVGIIGESGSGKTTLARAVAGMIAP----ARGSI 373 Query: 67 EVDGKDV---LKMSQRELAALRGDTVAMIFQEPLLALDPVYTVGAQIIEAIRRHKKVSKA 123 ++DG+ + L+ RE + V ++FQ AL+P +++G + + + + Sbjct: 374 QLDGETLSPTLEGRTRE----QFRRVQIVFQNADTALNPSHSIGRILNRPLSFYHGLKGE 429 Query: 124 EAHRQALSLFQKVRIPSPERRLDAYPHEMSGGMRQR 159 R+ L V++P+ +D P E+SGG +QR Sbjct: 430 AMRRRTAELLDLVQLPA--TVIDRLPAELSGGQKQR 463 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 464,775 Number of extensions: 17304 Number of successful extensions: 320 Number of sequences better than 1.0e-08: 28 Number of HSP's gapped: 314 Number of HSP's successfully gapped: 31 Length of query: 160 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 82 Effective length of database: 1,250,540 Effective search space: 102544280 Effective search space used: 102544280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)