BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0008.p (556 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310930|ref|YP_003775022.1| multicopper oxidase [Herbaspiri... 82 2e-16 >gi|300310930|ref|YP_003775022.1| multicopper oxidase [Herbaspirillum seropedicae SmR1] Length = 462 Score = 81.6 bits (200), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 48/122 (39%), Positives = 68/122 (55%), Gaps = 9/122 (7%) Query: 2 VTINVTNKLREHTSIHWHGIILPFQMDGVPGISFAGIAPGETFTYKFKVQQSGTYWYHSH 61 V I VTNKL E TS+HWHG LP MDGV G++ I PG+T+ Y+F +++GT+ YH H Sbjct: 116 VRIFVTNKLPEATSVHWHGQRLPNGMDGVSGLTQPAIPPGKTYVYEFTARRAGTFMYHPH 175 Query: 62 SG--MQEQTGMYGAIIIDPNGAGRSRGQGKNQADREHVLLLSDWMDEDPMSVLAKLKKQG 119 + +Q GM G + P G R DR+ V L++ + D P + + K+ Sbjct: 176 ADEMVQMAMGMMGFWVTHPKDPGFMR------VDRDFVFLMNAY-DIAPGASVPKVSTML 228 Query: 120 DF 121 DF Sbjct: 229 DF 230 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,729,357 Number of extensions: 74957 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 1 Length of query: 556 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 466 Effective length of database: 1,193,720 Effective search space: 556273520 Effective search space used: 556273520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)