BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0002.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 69 2e-13 gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis tra... 59 2e-10 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 58 4e-10 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 57 9e-10 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 68.9 bits (167), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 35/67 (52%), Positives = 45/67 (67%) Query: 1 MKNMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEWAANI 60 MKN++IG RL +GF +L L+A+M +GVWRL IG T M + L+KER A +W A I Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 61 DANGVRT 67 NGVRT Sbjct: 61 KENGVRT 67 Score = 67.0 bits (162), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 34/66 (51%), Positives = 44/66 (66%) Query: 75 MKNMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEWAANI 134 MKN++IG RL +GF +L L+A+M +GVWRL IG T M + L+KER A +W A I Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 135 DANGVR 140 NGVR Sbjct: 61 KENGVR 66 >gi|300310668|ref|YP_003774760.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 59.3 bits (142), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 26/56 (46%), Positives = 40/56 (71%) Query: 1 MKNMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEW 56 +K++RIGSRLG GFA+VL ++ VG+WRL+ + +T M+ + L+ ERL S+W Sbjct: 2 IKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQW 57 Score = 59.3 bits (142), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 26/56 (46%), Positives = 40/56 (71%) Query: 75 MKNMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEW 130 +K++RIGSRLG GFA+VL ++ VG+WRL+ + +T M+ + L+ ERL S+W Sbjct: 2 IKDIRIGSRLGAGFAVVLVFSMVVAAVGIWRLKTVAVQTQQMMDVPLKTERLVSQW 57 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 58.2 bits (139), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 30/69 (43%), Positives = 44/69 (63%), Gaps = 1/69 (1%) Query: 73 NQMKNMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEWAA 132 N + NM IG RL LGFAL+L+ ++ +G+WRL + T M+A L+ ERL ++W Sbjct: 2 NIIGNMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYT 61 Query: 133 NIDANGVRR 141 N+ A G+RR Sbjct: 62 NLVA-GIRR 69 Score = 55.5 bits (132), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 28/64 (43%), Positives = 41/64 (64%), Gaps = 1/64 (1%) Query: 3 NMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEWAANIDA 62 NM IG RL LGFAL+L+ ++ +G+WRL + T M+A L+ ERL ++W N+ A Sbjct: 6 NMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNLVA 65 Query: 63 NGVR 66 G+R Sbjct: 66 -GIR 68 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 57.0 bits (136), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 28/67 (41%), Positives = 40/67 (59%) Query: 1 MKNMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEWAANI 60 NM+IG RL LGFA++L+ +++ + +WRLQ I T M+ L KERL S+W I Sbjct: 4 FANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTI 63 Query: 61 DANGVRT 67 + RT Sbjct: 64 HTSVRRT 70 Score = 57.0 bits (136), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 29/67 (43%), Positives = 40/67 (59%), Gaps = 1/67 (1%) Query: 75 MKNMRIGSRLGLGFALVLSLLALMTIVGVWRLQDIGGRTATMIALDLQKERLASEWAANI 134 NM+IG RL LGFA++L+ +++ + +WRLQ I T M+ L KERL S+W I Sbjct: 4 FANMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTI 63 Query: 135 DANGVRR 141 VRR Sbjct: 64 H-TSVRR 69 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 465,214 Number of extensions: 15016 Number of successful extensions: 88 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 8 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)