BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0249.0011.p
         (391 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac...   699   0.0  
gi|300310489|ref|YP_003774581.1| glucarate dehydratase [Herbaspi...   177   1e-45

>gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate
           lactonizing [Herbaspirillum seropedicae SmR1]
          Length = 424

 Score =  699 bits (1805), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 343/424 (80%), Positives = 359/424 (84%), Gaps = 33/424 (7%)

Query: 1   MKITRVTVTPIAFKDAPLLNASGIHEPYALRSIIEVETDNGHFGLGESYGDAAALAVLDK 60
           MKITRVTVTPIAFKD PLLNASGIHEPYALRSIIE+ETDNG+ GLGESYGDA AL +LD 
Sbjct: 1   MKITRVTVTPIAFKDGPLLNASGIHEPYALRSIIEIETDNGYIGLGESYGDAPALKILDL 60

Query: 61  VKTQLIGLDPFNLNRLRAIVKSTVAGMAPAGNSGAELAPGSHASKAVSNAYSAFEVALLD 120
           VK +L+GLDPFNLN LRAIV+  VA MAPA N+GAELAPGSHASKAVSNAYSAFEVA LD
Sbjct: 61  VKERLVGLDPFNLNGLRAIVRGVVAAMAPASNAGAELAPGSHASKAVSNAYSAFEVACLD 120

Query: 121 AQARYLNVPLVDLLGGAVRTEIPFSAYLFFKYAEHIDSPYEPDSWGEALSEEQIVAQARS 180
           AQARYLNVPLVDLLGGAVR EIPFSAYLFFKYA+HIDSPY PD+WGEALSEEQIVAQAR 
Sbjct: 121 AQARYLNVPLVDLLGGAVRKEIPFSAYLFFKYAQHIDSPYAPDAWGEALSEEQIVAQARK 180

Query: 181 AY---------------------------------APLRIDPNGNWSLETAVRMAELLGD 207
                                              APLRIDPNGNWSL+TA+RMAELLGD
Sbjct: 181 MIEENGFQSIKLKAGALAPEHEVACIKALRKAFPDAPLRIDPNGNWSLQTAIRMAELLGD 240

Query: 208 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDEFRRSVAQNSVQIVLADHHYWGGL 267
           DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDE RRSVA +SVQIVLADHHYWGGL
Sbjct: 241 DLQYYEDPTPGLEGMAELHRRTGLPLATNMVVTDFDELRRSVALDSVQIVLADHHYWGGL 300

Query: 268 RDTQMLALMCDTFGLGVSMHSNSHLGISLMAMSHVAAAVPNLSYACDTHYPWQEADEEVI 327
           RDTQ LA MCD FGLGVSMHSNSHLGISLMAMSHVAAAV NLSYACDTHYPWQE DEEVI
Sbjct: 301 RDTQQLAKMCDVFGLGVSMHSNSHLGISLMAMSHVAAAVENLSYACDTHYPWQEPDEEVI 360

Query: 328 KGGKLSIRNGCVGITRAPGLGVELDRDQLAKLHDLYLSCGIRSRDDVRQMRKYKPDWKTV 387
           +GGKL IRNGCV IT APGLGVE+D+DQL KLH LYL CGIR RDDV QMRKY+PDWKTV
Sbjct: 361 QGGKLPIRNGCVQITDAPGLGVEVDQDQLRKLHQLYLECGIRQRDDVGQMRKYQPDWKTV 420

Query: 388 KPRF 391
           KPRF
Sbjct: 421 KPRF 424


>gi|300310489|ref|YP_003774581.1| glucarate dehydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 455

 Score =  177 bits (449), Expect = 1e-45,   Method: Compositional matrix adjust.
 Identities = 143/435 (32%), Positives = 205/435 (47%), Gaps = 59/435 (13%)

Query: 2   KITRVTVTPIAFKDAPLLNASGIHEPYALRSIIEVETDNGHFGLGESYGDAAALAVLDKV 61
           ++T + V P+A  D+ LLN SG H PY  R+++ +    G+ G+GE  G       L+  
Sbjct: 19  RVTEMRVIPVAGHDSMLLNLSGAHGPYFTRNLVILTDSAGNTGVGEVPGGEGIRQTLEDA 78

Query: 62  KTQLIGLDPFNLNRLRAIVKSTVAGMAPAGNSGAELAPGSHASKAVSNAYSAFEVALLDA 121
           +T ++G    ++  ++ I+       A     G  L   +   +   +A +A E ALLD 
Sbjct: 79  RTLVVGQ---SIGNVQGILNRARTAFADRDTGGRGLQ--TFDLRIAIHAVTALEAALLDL 133

Query: 122 QARYLNVPLVDLLG-GAVRTEIPFSAYLFFKYAEH-IDSPY-----EPDSWGE-----AL 169
             ++L+VP+  LLG G  R E+    YLF+    +  D PY       D W       AL
Sbjct: 134 LGKFLDVPVAALLGEGQQRDEVEMLGYLFYVADRNKTDLPYLAAPDAQDDWTRLRHEAAL 193

Query: 170 SEEQIVAQARSAYAP---------------------------------LRIDPNGNWSLE 196
           + E +V  A +AYA                                  + +DPNG W L+
Sbjct: 194 TPEAVVRLAEAAYARYGFNDFKLKGGVMRGEEEIAAVTALAERFPKARITLDPNGGWLLK 253

Query: 197 TAVRMAELLGDDLQYYEDPT------PGLEGMAELHRRTGLPLATNMVVTDFDEFRRSVA 250
            A+R+     D L Y EDP        G E MAE  R TGL  ATNMV TD+ +   +++
Sbjct: 254 DAIRLCRDQHDVLAYAEDPCGAEDGYSGREVMAEFRRATGLQTATNMVATDWRQMGHAIS 313

Query: 251 QNSVQIVLADHHYWGGLRDTQMLALMCDTFGLGVSMHSNSHLGISLMAMSHVAAAVPNLS 310
             SV I LAD H+W  ++ +  +A MC  +GL    HSN+H  +SL   +HVAAA P   
Sbjct: 314 LQSVDIPLADPHFW-TMQGSVRVAQMCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKI 372

Query: 311 YACDTHYPWQEADEEVIKGGKLSIRNGCVGITRAPGLGVELDRDQLAKLHDLYLSCGIRS 370
            A DTH+ WQ  D + +    L I  G V +   PGLGVELD  Q+   H  Y + G+ +
Sbjct: 373 TAIDTHWIWQ--DGQRLTREPLQIVGGKVQVPTKPGLGVELDMAQVEAAHQTYRNMGLGA 430

Query: 371 RDDVRQMRKYKPDWK 385
           RDD   M+   P WK
Sbjct: 431 RDDAVAMQYLIPGWK 445


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.135    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,270,905
Number of extensions: 54168
Number of successful extensions: 162
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 159
Number of HSP's successfully gapped: 4
Length of query: 391
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 304
Effective length of database: 1,207,925
Effective search space: 367209200
Effective search space used: 367209200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)