BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0249.0009.p
         (350 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ...   277   7e-76
gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge...    72   8e-14

>gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase
           large subunit [Herbaspirillum seropedicae SmR1]
          Length = 922

 Score =  277 bits (709), Expect = 7e-76,   Method: Compositional matrix adjust.
 Identities = 132/156 (84%), Positives = 141/156 (90%)

Query: 1   MGVNQSSAGTDKVNSIINCHLISGRIGKPGMGPFSITGQPNAMGGREVGGLANMLAAHMD 60
           MGVNQSSAGTDKVNSIINCHL +GRIGKPGMGPFSITGQPNAMGGREVGGLANMLAAHMD
Sbjct: 307 MGVNQSSAGTDKVNSIINCHLATGRIGKPGMGPFSITGQPNAMGGREVGGLANMLAAHMD 366

Query: 61  LDNALHRDTVQTFWGSPHMADKPGLKAVDLFNAIEAGRIKAVWIMATNPMVSMPDANVVQ 120
           LD  LHR  VQ FW SP +ADKPGLKAVD+F AIE GRIKAVWIMATNPMVS+PDA++VQ
Sbjct: 367 LDQPLHRQIVQDFWQSPRIADKPGLKAVDMFRAIEEGRIKAVWIMATNPMVSLPDADLVQ 426

Query: 121 RALSKCELVVASDIVEGTDTNAFAHVLLPALGWGEK 156
           RAL KCELVV+SD++  TDTNA AHVLLPAL WGEK
Sbjct: 427 RALQKCELVVSSDVITQTDTNALAHVLLPALAWGEK 462



 Score =  262 bits (669), Expect = 3e-71,   Method: Compositional matrix adjust.
 Identities = 123/172 (71%), Positives = 142/172 (82%), Gaps = 1/172 (0%)

Query: 142 AFAHVLLPALGWGEKKKXDARVGALVNPVVDPLSGEPEFKHTPVSIEEFGVSWHGFILSR 201
           A   V +P + W  +   DARVGA+VNPVVDP+SGEPEFKHTPV+IEEF VSWHGFIL+R
Sbjct: 677 ARGSVFVP-IHWNGQSASDARVGAVVNPVVDPVSGEPEFKHTPVAIEEFRVSWHGFILTR 735

Query: 202 KTLALDEITHWTRIQGRDFARYELAGRSTIADHSAWARKLLGVHDIEADWLEYEDRTAGV 261
             L LD++THWTR+QGRDF RYELAGR+ I DHSAWAR LLGV D +ADWLEYED + G 
Sbjct: 736 HALDLDQVTHWTRVQGRDFMRYELAGRNHIPDHSAWARSLLGVQDPQADWLEYEDLSDGS 795

Query: 262 YRAVHVENERIDMCIFLSKRPDLPSRAWLASLFDKEQLEEADRVGLLIGQGI 313
           YRAVHV  +RIDMC+F+S RPDLPSR+WLA LF KE+LEEADRVGLL+GQ I
Sbjct: 796 YRAVHVVEDRIDMCLFMSARPDLPSRSWLAGLFAKEKLEEADRVGLLVGQPI 847


>gi|300310808|ref|YP_003774900.1| NAD-dependent formate
           dehydrogenase subunit alpha [Herbaspirillum seropedicae
           SmR1]
          Length = 957

 Score = 71.6 bits (174), Expect = 8e-14,   Method: Compositional matrix adjust.
 Identities = 50/195 (25%), Positives = 94/195 (48%), Gaps = 7/195 (3%)

Query: 1   MGVNQSSAGTDKVNSIINCHLISGRIGKPGMGPFSITGQPNAMGGREVGGLANMLAAHMD 60
           +GV + + G+  V  I N  + +G +G+ G+G   + GQ N  G  ++G   + L  +  
Sbjct: 541 LGVTEHAQGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRH 600

Query: 61  LDNALHRDTVQTFWGSPHMADKPGLKAVDLFNAIEAGRIKAVWIMATNPMVSMPDANVVQ 120
           + ++  R+  +  WG   +  +PGL+  ++F+A   G  K ++    + + S P+   V 
Sbjct: 601 ISDSTVRNQFEQAWGVT-LNPEPGLRIPNMFDAALDGSFKGLYCEGEDIVQSDPNTQHVA 659

Query: 121 RALSKCELVVASDIVEGTDTNAFAHVLLPALGWGEK----KKXDARVGALVNPVVDPLSG 176
            AL   E +V  D+    +T  +AHV LP   + EK       + R+ + V  V+ P +G
Sbjct: 660 AALQAMECIVVQDLFL-NETAKYAHVFLPGSSFLEKDGTFTNAERRI-SRVRKVMPPKAG 717

Query: 177 EPEFKHTPVSIEEFG 191
           + +++ T    E  G
Sbjct: 718 KSDWEVTVALAEALG 732


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.137    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,185,833
Number of extensions: 51937
Number of successful extensions: 131
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 130
Number of HSP's successfully gapped: 3
Length of query: 350
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 264
Effective length of database: 1,212,660
Effective search space: 320142240
Effective search space used: 320142240
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)