BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0006.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleoti... 536 e-153 gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit... 154 9e-39 gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspi... 56 4e-09 >gi|300312207|ref|YP_003776299.1| FAD-dependent pyridine nucleotide-disulfide oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 536 bits (1380), Expect = e-153, Method: Compositional matrix adjust. Identities = 263/312 (84%), Positives = 281/312 (90%), Gaps = 1/312 (0%) Query: 14 SAKKPSLVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKSID 73 S +PSLVV+GNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRI+LSPVLAGDKSID Sbjct: 8 STARPSLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRIMLSPVLAGDKSID 67 Query: 74 DIMLNTRDWYVQNNITLHAGDPVERIDRRRRIVRSQAGIEIEYDRLLLATGSKPFIIPVP 133 DIMLN R WY N ITLHAGDPV IDRR+R V +++G+ ++YDRLLLATGS PFIIPVP Sbjct: 68 DIMLNPRAWYDDNGITLHAGDPVVHIDRRQRTVHAKSGLTVQYDRLLLATGSTPFIIPVP 127 Query: 134 GHELPGVIAFRDIQDVQTMLDAARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHVTDS 193 GH+LPGVIAFRDIQDV ML AAR HRHAVVIGGGLLGLEAANGL+RQGMDVTVVHVTD+ Sbjct: 128 GHQLPGVIAFRDIQDVDAMLQAARTHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHVTDA 187 Query: 194 LMNQQLDKPAAALLKKALEMKGLRFLLNAQTDAIVGPDRVTAVRFKDGSEIPADLVVMTA 253 LMNQQLDKPAAALLKKALE KGLRFLLNA T+ IVGPDRVTAVRFKDGS IPADLVVMTA Sbjct: 188 LMNQQLDKPAAALLKKALEDKGLRFLLNAHTEEIVGPDRVTAVRFKDGSSIPADLVVMTA 247 Query: 254 GVRPNISLAKDAGLHCDRAIVVDDTLQTYDPRIYAVGECVQHRKATFGLVAPIWDQARXS 313 GVRPNI+LAK AGLHC+RAI+VDDTLQTYDPR+YAVGECVQHRKATFGLVAPIWDQAR Sbjct: 248 GVRPNIALAKAAGLHCERAIIVDDTLQTYDPRVYAVGECVQHRKATFGLVAPIWDQARVC 307 Query: 314 APRHLQAAGAAR 325 HL AG R Sbjct: 308 GA-HLAGAGHRR 318 >gi|300312205|ref|YP_003776297.1| nitrite reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 857 Score = 154 bits (389), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 112/311 (36%), Positives = 175/311 (56%), Gaps = 16/311 (5%) Query: 20 LVVIGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGDKSIDDIMLNT 79 ++VIG+GM G + +E L + +T+ EP Y+R+ LS AG K+ +D+ L Sbjct: 3 IIVIGHGMVGHKFLECLAESGAGDLQVTILCEEPRPAYDRVHLSEFFAG-KTAEDLSLVA 61 Query: 80 RDWY------VQNNITLHAGDPVERID-RRRRIVRSQAGIE--IEYDRLLLATGSKPFII 130 ++ VQ ++ L+A ++ID R++++ + AG+E + YD+L+LATGS PF+ Sbjct: 62 PGFFDRGRSAVQFDLKLNA--KAQQIDLERKQVLVNVAGVEETMSYDKLVLATGSYPFVP 119 Query: 131 PVPGHELPGVIAFRDIQDVQTMLDAARNHRHAVVIGGGLLGLEAANGLLRQGMDVTVVHV 190 V G++ +R I+D++ ML+ + VVIGGGLLGLE A L ++ VV Sbjct: 120 TVAGNQRKDCFVYRTIEDLEAMLECGSRSKIGVVIGGGLLGLECAKALRDLKLETHVVEF 179 Query: 191 TDSLMNQQLDKPAAALLKKALEMKGLRFLLNAQTDAIV-GPDRVTAVRFKDGSEIPADLV 249 LM Q+D+ +L++ +E G+ T IV G + F+DGS + D++ Sbjct: 180 APRLMAVQVDESGGRVLRQKIEELGVTAHTQKNTVEIVDGKHHTHRMNFEDGSHLETDMI 239 Query: 250 VMTAGVRPNISLAKDAGLHC-DR-AIVVDDTLQTYDPRIYAVGECVQHRKATFGLVAPIW 307 V +AG+RP LA+ AGL DR I +D++ T DP +YA+GEC FGLVAP + Sbjct: 240 VFSAGIRPRDELARQAGLKVGDRGGIAIDNSCLTSDPAVYAIGECALWNGKIFGLVAPGY 299 Query: 308 DQARXSAPRHL 318 D AR +A +HL Sbjct: 300 DMAR-TAAKHL 309 >gi|300312608|ref|YP_003776700.1| glutathione reductase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 55.8 bits (133), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 53/186 (28%), Positives = 88/186 (47%), Gaps = 20/186 (10%) Query: 119 LLLATGSKPFIIPVPGHELPGVIAFRDIQDVQTMLDAARNHRHAVVIGGGLLGLEAANGL 178 +L+ATG +P +PG EL I D ++ + + AVV+GGG + +E A+ L Sbjct: 136 ILVATGGRPDKPAIPGAELG--ITSDDFFHLKAL------PQRAVVLGGGYIAVELASIL 187 Query: 179 LRQGMDVTVVHVTDSLMNQQLDKPAAALLKKALEMKGLRFLLNAQTDAI-------VGPD 231 G +VT+V+ + L+ + +D L + KG+R + NA +AI Sbjct: 188 NGLGCEVTLVYRRERLL-RNMDADLGIHLADEMAKKGIRIVFNASIEAIEAEQAGEASEA 246 Query: 232 RVTAVRFKDGSEIPADLVVMTAGVRPNIS--LAKDAG--LHCDRAIVVDDTLQTYDPRIY 287 V VR +G + A V+ G N + ++AG L + AI V+ ++ P I Sbjct: 247 SVKTVRLTNGEALSAQCVLFATGRTANTAGLGLQEAGVKLKANGAIEVNQDFESSVPSIL 306 Query: 288 AVGECV 293 AVG+ + Sbjct: 307 AVGDVI 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 956,156 Number of extensions: 36417 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 131 Number of HSP's successfully gapped: 3 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)