BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0005.p (127 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312206|ref|YP_003776298.1| nitrite reductase small subunit... 174 3e-45 >gi|300312206|ref|YP_003776298.1| nitrite reductase small subunit [Herbaspirillum seropedicae SmR1] Length = 120 Score = 174 bits (440), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 82/123 (66%), Positives = 102/123 (82%), Gaps = 5/123 (4%) Query: 7 MHSDRQLNHCEHWTEVCDIADIVPNTGVCALIGSEQVAVFHVIDGAHNET--RVFAIGNY 64 MHSD+Q+ +HWT VC++ +IVPNTGV AL+G +QVAVFHVID + E RVFA+GNY Sbjct: 1 MHSDQQI---KHWTPVCELGEIVPNTGVAALVGDQQVAVFHVIDRSQGEPQPRVFAVGNY 57 Query: 65 DPNAEASVLSRGLVGSIGERIVVASPIYKQHFDLQTGECIEAPEHSVSAYPARVEHGKVW 124 DPN +A+VLSRGLVG++GERIVVASPIYK HFDL TGEC+E P +S+ A+PAR+ GKVW Sbjct: 58 DPNCDAAVLSRGLVGNLGERIVVASPIYKHHFDLTTGECLEEPANSIPAWPARIADGKVW 117 Query: 125 VGA 127 +GA Sbjct: 118 IGA 120 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 408,270 Number of extensions: 15409 Number of successful extensions: 36 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 35 Number of HSP's successfully gapped: 1 Length of query: 127 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 52 Effective length of database: 1,264,745 Effective search space: 65766740 Effective search space used: 65766740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)