BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0248.0016.p (324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptid... 506 e-144 gi|300309747|ref|YP_003773839.1| membrane metalloendopeptidase [... 77 1e-15 gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 68 1e-12 gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 67 2e-12 gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 62 4e-11 >gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 506 bits (1303), Expect = e-144, Method: Compositional matrix adjust. Identities = 252/325 (77%), Positives = 293/325 (90%), Gaps = 1/325 (0%) Query: 1 MQIILLHPRFTRARSVTLTSKHVVAVLLFLVVSIAASAAGLSYLVLRHASDSDSSPMLHK 60 MQIILLHPRFT+ARS+TL ++H++ +LL L+ S+ + +SYL+LR A D ++SPMLHK Sbjct: 1 MQIILLHPRFTKARSITLGTRHLLLLLLLLIGSVIGCSMLISYLMLRQAGDPEASPMLHK 60 Query: 61 LMVSMTQQEDDRKDKYLKENLAVMAVKLGEMQAQLMRLDALGERVQGLAGVRPEEFNFKD 120 L SM+Q+EDDRK+K++KENLA+MAVK+GEMQAQ+MRLDALGERVQGLAG+RPEEFNFK+ Sbjct: 61 LAASMSQEEDDRKEKFVKENLAMMAVKVGEMQAQMMRLDALGERVQGLAGIRPEEFNFKE 120 Query: 121 KPGRGGVETSSIGGPGRELGMSELQKMLDALSVDVGHRADYMNAVESTLMGDKIKSRLLP 180 PGRGG E SS+ GPGR++G+ EL++MLD L+VD GHR DY+NAVESTLMGDKIKSRLLP Sbjct: 121 LPGRGGAEVSSLAGPGRDVGLDELRRMLDNLAVDAGHRTDYLNAVESTLMGDKIKSRLLP 180 Query: 181 TNPPVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPASTGTSIVAAAAGVVIAAEYHYQFGN 240 TN PVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPA GT IVAAAAGVVIA+EYHYQFGN Sbjct: 181 TNQPVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPAPVGTRIVAAAAGVVIASEYHYQFGN 240 Query: 241 MLEIDHGNDIITRYAHASQLFVKVGDIVRRGQHVADIGTTGRSTGPHLHFEVRIRGVAQD 300 MLEIDHGN+IITRYAHAS+LFVKVGDIV+RGQHVAD+G+TGRSTG HLHFEVRIRG+AQD Sbjct: 241 MLEIDHGNNIITRYAHASRLFVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGIAQD 300 Query: 301 PRKFLALGSPGGG-PRAAPVFVAGR 324 PRKFLALG+ GG P+ PVFVAGR Sbjct: 301 PRKFLALGANGGNQPKQMPVFVAGR 325 >gi|300309747|ref|YP_003773839.1| membrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 49/133 (36%), Positives = 67/133 (50%), Gaps = 4/133 (3%) Query: 171 GDKIKSRLLPTNPPVNVAYNSSSFGWRLDPFTGHNAFHEGLDFPASTGTSIVAAAAGVVI 230 G +K L + P+ SS F RL P G H G+DF A TGT I AAA GVV Sbjct: 329 GKSLKKAFLKS--PLAFTRISSGFSMRLHPILGKWKQHTGVDFAAPTGTPIHAAADGVVD 386 Query: 231 AAEYHYQFGNMLEIDHGNDIITRYAHASQLF--VKVGDIVRRGQHVADIGTTGRSTGPHL 288 +GN++ I H + T Y H S+ +K G V + + +G TG +TGPHL Sbjct: 387 FVGKQNGYGNIVVIKHWSGYSTAYGHMSRFAPDIKKGMKVSQNDVIGFVGMTGWATGPHL 446 Query: 289 HFEVRIRGVAQDP 301 H+E R+ ++P Sbjct: 447 HYEFRVNNQPRNP 459 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 67.8 bits (164), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 34/96 (35%), Positives = 56/96 (58%) Query: 210 GLDFPASTGTSIVAAAAGVVIAAEYHYQFGNMLEIDHGNDIITRYAHASQLFVKVGDIVR 269 GL A G + A AAG V+ A++ FGN++ +DHGN +T Y + + + GD+V+ Sbjct: 372 GLFIRAHEGAEVRAVAAGRVVFADWLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDLVK 431 Query: 270 RGQHVADIGTTGRSTGPHLHFEVRIRGVAQDPRKFL 305 G +A G +G + L+FE+R +G A DP+ ++ Sbjct: 432 AGDTIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWV 467 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 35/99 (35%), Positives = 59/99 (59%), Gaps = 2/99 (2%) Query: 208 HEGLDFPASTGTSIVAAAAGVVIAAEYHYQ-FGNMLEIDHGNDIITRYAHASQLFVKVGD 266 +G+D G +++AA AG V+ A + +GN++ I H +++++ YAH + VK G Sbjct: 232 KKGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLVIIKHTSNLLSAYAHNQTILVKEGQ 291 Query: 267 IVRRGQHVADIGTTGRSTGPHLHFEVRIRGVAQDPRKFL 305 V +GQ +A++G + T LHFE+R +G DP K+L Sbjct: 292 SVTKGQKIAEMGKSDSDT-VKLHFEIRQQGKPVDPSKYL 329 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 35/93 (37%), Positives = 55/93 (59%), Gaps = 2/93 (2%) Query: 205 NAFHEGLDFPASTGTSIVAAAAGVVIAAEYHYQ-FGNMLEIDHGNDIITRYAHASQLFVK 263 +++ +G+D S G S+VAAAAG V A + + +GNM+ I H +++ YAH + VK Sbjct: 151 DSYKKGIDIIGSEGQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSVYAHNKTILVK 210 Query: 264 VGDIVRRGQHVADIGTTGRSTGPHLHFEVRIRG 296 G V RGQ +A +G + + L+FE+R G Sbjct: 211 EGQTVTRGQQIATMGNSD-TNKVKLYFEIRRNG 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 991,776 Number of extensions: 40104 Number of successful extensions: 124 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 121 Number of HSP's successfully gapped: 5 Length of query: 324 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 239 Effective length of database: 1,217,395 Effective search space: 290957405 Effective search space used: 290957405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)