BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0248.0013.p (106 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313911|ref|YP_003778003.1| cations and cationic drugs memb... 59 7e-11 >gi|300313911|ref|YP_003778003.1| cations and cationic drugs membrane transport protein [Herbaspirillum seropedicae SmR1] Length = 110 Score = 58.9 bits (141), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 38/105 (36%), Positives = 61/105 (58%) Query: 2 AWIILVLAGLFEVAWAVGLKYTDGFTRLLPSVATLAAMVVSVGLLALAVKTLPLSTAYAI 61 A++ L +A + EV LK + GFTRLLP + +A VS LAL ++T+P S AYAI Sbjct: 3 AYLSLAIAIVAEVIATTALKSSQGFTRLLPGLIVVAGYGVSFYCLALTLRTMPTSVAYAI 62 Query: 62 WTGIGAVGAVVLGIVLFHESASPARLACLALIIVGIIGLKVVSPE 106 W+G+G V + ++ H+ +A +ALI+ G++ + + S Sbjct: 63 WSGVGLVLITLAAWLIHHQRLDWPAVAGIALIVAGVMVINLFSSS 107 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 254,536 Number of extensions: 7546 Number of successful extensions: 52 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 52 Number of HSP's successfully gapped: 1 Length of query: 106 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 34 Effective length of database: 1,278,950 Effective search space: 43484300 Effective search space used: 43484300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 123 (52.0 bits)