BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0248.0010.p (61 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309689|ref|YP_003773781.1| transmembrane protein [Herbaspi... 94 3e-21 >gi|300309689|ref|YP_003773781.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 275 Score = 93.6 bits (231), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 41/59 (69%), Positives = 48/59 (81%) Query: 3 IAGHCDLVYEIHPERGWAGVIGGNVFNSVSESLIPIDANARLLPLARRPWFVVAKNLMP 61 +AGHCD+V E+H + GWAGVIGGNVFNSVSESLIP+D R P+A RPW VV KNL+P Sbjct: 217 LAGHCDVVVEVHADDGWAGVIGGNVFNSVSESLIPVDGQGRAQPIAARPWLVVVKNLLP 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.142 0.466 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 193,780 Number of extensions: 5933 Number of successful extensions: 19 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 19 Number of HSP's successfully gapped: 1 Length of query: 61 Length of database: 1,619,870 Length adjustment: 33 Effective length of query: 28 Effective length of database: 1,463,615 Effective search space: 40981220 Effective search space used: 40981220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 123 (52.0 bits)