BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0002.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313080|ref|YP_003777172.1| major facilitator superfamily p... 155 2e-39 >gi|300313080|ref|YP_003777172.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 403 Score = 155 bits (391), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 104/162 (64%), Positives = 119/162 (73%), Gaps = 6/162 (3%) Query: 8 DARAPVLRKDS-----HRWKILGIGVLANASFSAAFAGIPVTAIFMRADYRLDNTQLGLA 62 A APVL + S HRWK+L GV ANASFSA F+GIPVT++ +R+DYRL NTQLGLA Sbjct: 2 QAAAPVLPRPSPSPSPHRWKVLAAGVAANASFSAVFSGIPVTSVVLRSDYRLSNTQLGLA 61 Query: 63 LGSLGLGVAVSELPWGVLTDRWGDRRVLLCGLISTALALAAMAACVAPDAAGVPSLWLLA 122 LG L LGVA+ ELPWGVLTDRWGDR+VLL GL+ + AL + P WL A Sbjct: 62 LGLLSLGVALGELPWGVLTDRWGDRKVLLAGLLGASAALGLLMLYALPGQGAASFAWLCA 121 Query: 123 LGLLTVGLLGGSVNGSSGRAVMGWFREGERGLAMSIRQTAVP 164 L+ GLLGGSVNG+SGRA+MGWF EGERGLAMSIRQTAVP Sbjct: 122 G-LMLAGLLGGSVNGASGRAIMGWFGEGERGLAMSIRQTAVP 162 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 377,425 Number of extensions: 10133 Number of successful extensions: 65 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 1 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)