BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0246.0009.p (363 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314057|ref|YP_003778149.1| methyl-accepting chemotaxis pro... 476 e-135 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 345 5e-96 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 336 1e-93 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 333 1e-92 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 332 4e-92 >gi|300314057|ref|YP_003778149.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 554 Score = 476 bits (1226), Expect = e-135, Method: Compositional matrix adjust. Identities = 258/333 (77%), Positives = 281/333 (84%), Gaps = 2/333 (0%) Query: 10 GVVLATLITRHIRRALGAEPAQVKELAFAVDRGELYHAIEQDKDLGKGR-ESIMAALSEM 68 G VLA LITR IR ALGAEP +VKELA AVDRGELYHA GK R SIMA LSEM Sbjct: 211 GTVLAMLITRSIRGALGAEPDEVKELALAVDRGELYHAAALPL-AGKTRARSIMATLSEM 269 Query: 69 SSNLRRTVTEVRDAASDVTEISAQIAEGNRDLAARTEDQAASLEETASAMEQLTSTVKQN 128 S NLR TVT VRDAA V IS+QIA GNRDLAARTEDQAASLEETASAMEQLT+TVKQN Sbjct: 270 SGNLRHTVTRVRDAADGVATISSQIAAGNRDLAARTEDQAASLEETASAMEQLTATVKQN 329 Query: 129 DDNAKQANQLARNASAIAIQGGAIVGQVVHTMDSINTSSRKIVDIIGVIDGIAFQTNILA 188 D++A+QANQLA NAS IA QGGAIV VV TM +IN SSRKIVDIIGVIDGIAFQTNILA Sbjct: 330 DNHARQANQLAHNASEIARQGGAIVDNVVDTMAAINVSSRKIVDIIGVIDGIAFQTNILA 389 Query: 189 LNAAVEAARAGEQGRGFAVVATEVRSLAQRSSAAAKEIKSLIDDSVEKVDTGAKLVGQAG 248 LNAAVEAARAGEQGRGFAVVATEVR+LAQRS+AAAKE+K LIDDSV+ V+ G LV QAG Sbjct: 390 LNAAVEAARAGEQGRGFAVVATEVRNLAQRSAAAAKEVKQLIDDSVQNVENGTHLVEQAG 449 Query: 249 ETMEQIVASVKHVTDVVGEISAASHEQSIGIEEVHRAIALMDQVTQQNAALVEQASAAVG 308 +TMEQIVASV+ VTDV+GEIS+ASHEQS+GIEEVH+AIALMDQVTQ NAALVEQASAAV Sbjct: 450 QTMEQIVASVQQVTDVMGEISSASHEQSLGIEEVHKAIALMDQVTQHNAALVEQASAAVA 509 Query: 309 TLQDQAGRLNQAVGVFSLEDPALSSSARILPLR 341 TLQ+QA LNQAVGVF LE P S + R + ++ Sbjct: 510 TLQEQAVNLNQAVGVFQLERPDQSPTLRTINVK 542 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 345 bits (884), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 209/336 (62%), Positives = 252/336 (75%), Gaps = 8/336 (2%) Query: 6 AMATGVVLATLITRHIRRALGAEPAQVKELAFAVDRGELYHAIEQDKDLGKGRESIMAAL 65 A+ ++L L+ R I + L E +V E V G+L IE G+ +M AL Sbjct: 202 AVLAAIILGYLMARSIVKPL-TEAVRVAE---NVAAGDLTTRIEPHSRDETGQ--LMQAL 255 Query: 66 SEMSSNLRRTVTEVRDAASDVTEISAQIAEGNRDLAARTEDQAASLEETASAMEQLTSTV 125 +M+ NL V+ VR +T S +IA GN DL+ARTE QA SLEETASAME++TSTV Sbjct: 256 RKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEETASAMEEMTSTV 315 Query: 126 KQNDDNAKQANQLARNASAIAIQGGAIVGQVVHTMDSINTSSRKIVDIIGVIDGIAFQTN 185 +QN DNA+QANQ+A +AS IA+QGG IVG+VVHTMD IN SSRKIVDIIGVIDGIAFQTN Sbjct: 316 RQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDEINQSSRKIVDIIGVIDGIAFQTN 375 Query: 186 ILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSSAAAKEIKSLIDDSVEKVDTGAKLVG 245 ILALNAAVEAARAGEQGRGFAVVATEVRSLAQRS+AAAKEIK+LI DSV KV+ G+ LV Sbjct: 376 ILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKALIADSVGKVEGGSALVA 435 Query: 246 QAGETMEQIVASVKHVTDVVGEISAASHEQSIGIEEVHRAIALMDQVTQQNAALVEQASA 305 QAG TMEQ+V+SV+ VTD+V EISAAS EQS GIEE++RA+ MD+ TQQNAALVEQA+A Sbjct: 436 QAGATMEQVVSSVRSVTDIVAEISAASAEQSSGIEEINRAVTQMDEGTQQNAALVEQAAA 495 Query: 306 AVGTLQDQAGRLNQAVGVFSLEDPALSSSARILPLR 341 A ++Q+QA RL Q++ +F LE A S A P+R Sbjct: 496 AAKSMQEQAVRLTQSISIFKLEQGAASIPA--APVR 529 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 336 bits (862), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 210/352 (59%), Positives = 260/352 (73%), Gaps = 16/352 (4%) Query: 1 VLGAIAMATGVVLATLITRHIRRALGAEPAQVKELAFAVDRGELYHAIEQDKDLGKGRES 60 VL +A+ + A LITR I R L + ++A AV G+L IE G + Sbjct: 195 VLSGVAVLLSALCAMLITRSITRPLN----EAVQVATAVAAGDLTVQIEATSKDETG--A 248 Query: 61 IMAALSEMSSNLRRTVTEVRDAASDVTEISAQIAEGNRDLAARTEDQAASLEETASAMEQ 120 ++ AL M+ NL R V EVR + S +IA GN DL++RTE QA +LEETASAME+ Sbjct: 249 LLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGALEETASAMEE 308 Query: 121 LTSTVKQNDDNAKQANQLARNASAIAIQGGAIVGQVVHTMDSINTSSRKIVDIIGVIDGI 180 LTSTV+QN DNA+QAN LA AS +AIQGG++VG+VV TM IN +SRKIVDII VIDGI Sbjct: 309 LTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIVDIISVIDGI 368 Query: 181 AFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSSAAAKEIKSLIDDSVEKVDTG 240 AFQTNILALNAAVEAARAGEQGRGFAVVA+EVR+LAQRS+AAAKEIKSLID SVE+V++G Sbjct: 369 AFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLIDASVERVESG 428 Query: 241 AKLVGQAGETMEQIVASVKHVTDVVGEISAASHEQSIGIEEVHRAIALMDQVTQQNAALV 300 ++LVGQAG TME++VASVK VTDVV EI+AAS EQS GIE++++AI MD+VTQQNAALV Sbjct: 429 SRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQAIVQMDEVTQQNAALV 488 Query: 301 EQASAAVGTLQDQAGRLNQAVGVFSL------EDPALSS----SARILPLRP 342 EQA+AA +LQ+Q+GRL+ VG+F L + LS+ S ++ P RP Sbjct: 489 EQAAAAAQSLQEQSGRLSDTVGIFRLGNDHAHQPSPLSAPVQRSVQVAPARP 540 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 333 bits (854), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 202/325 (62%), Positives = 250/325 (76%), Gaps = 6/325 (1%) Query: 2 LGAIAMATGVVLATLITRHIRRALGAEPAQVKELAFAVDRGELYHAIEQDKDLGKGRESI 61 L A+A+A V+ A L+TR I R L + +A AV G+L I G ++ Sbjct: 195 LSAVAIALCVIAAWLVTRSITRPLN----EAVSVASAVAAGDLTVQIGATSKDETG--AL 248 Query: 62 MAALSEMSSNLRRTVTEVRDAASDVTEISAQIAEGNRDLAARTEDQAASLEETASAMEQL 121 +AAL M+ +L R V+EVR + + SA+IA GN DL++RTE+QA +LEETASAME+L Sbjct: 249 LAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAGALEETASAMEEL 308 Query: 122 TSTVKQNDDNAKQANQLARNASAIAIQGGAIVGQVVHTMDSINTSSRKIVDIIGVIDGIA 181 TSTVKQN DNA+QAN LA AS +A+QGG++VGQVV TM IN +SRKIVDII VIDGIA Sbjct: 309 TSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRKIVDIISVIDGIA 368 Query: 182 FQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSSAAAKEIKSLIDDSVEKVDTGA 241 FQTNILALNAAVEAARAGEQGRGFAVVA+EVR+LAQRS++AAKEIK+LIDDSV +VD G+ Sbjct: 369 FQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKALIDDSVSRVDNGS 428 Query: 242 KLVGQAGETMEQIVASVKHVTDVVGEISAASHEQSIGIEEVHRAIALMDQVTQQNAALVE 301 +LV QAG TM ++VASV+ VTDVV EISAAS+EQS GIE+++ AI MDQVTQQNAALVE Sbjct: 429 RLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQMDQVTQQNAALVE 488 Query: 302 QASAAVGTLQDQAGRLNQAVGVFSL 326 +A+AA +LQ+Q+ RL+ V VF L Sbjct: 489 EAAAAAQSLQEQSTRLSATVSVFKL 513 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 332 bits (850), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 204/329 (62%), Positives = 242/329 (73%), Gaps = 6/329 (1%) Query: 2 LGAIAMATGVVLATLITRHIRRALGAEPAQVKELAFAVDRGELYHAIEQDKDLGKGRESI 61 LGAI + G LA L+TR I R L Q +A V G+L I+ G+ + Sbjct: 193 LGAIEIVLGAALAWLLTRSITRPLN----QAVTIAETVAAGDLTSRIDSSSRDETGK--L 246 Query: 62 MAALSEMSSNLRRTVTEVRDAASDVTEISAQIAEGNRDLAARTEDQAASLEETASAMEQL 121 +AAL M+ NL V VR + S +IA GN DL+ARTE QA SLEETASAMEQL Sbjct: 247 LAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETASAMEQL 306 Query: 122 TSTVKQNDDNAKQANQLARNASAIAIQGGAIVGQVVHTMDSINTSSRKIVDIIGVIDGIA 181 TSTVKQN DNA+QAN+LA +AS +A QGGA+V QVV TM +I+ SS+KI DIIGVIDGIA Sbjct: 307 TSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIGVIDGIA 366 Query: 182 FQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSSAAAKEIKSLIDDSVEKVDTGA 241 FQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRS++AAKEIK LIDDSVEKVD G+ Sbjct: 367 FQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKVDDGS 426 Query: 242 KLVGQAGETMEQIVASVKHVTDVVGEISAASHEQSIGIEEVHRAIALMDQVTQQNAALVE 301 +LV +AG+TM+++V SV VT VV EI+ AS EQS GIE+V+ AI +D+ TQQNAALVE Sbjct: 427 RLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQNAALVE 486 Query: 302 QASAAVGTLQDQAGRLNQAVGVFSLEDPA 330 QA+AA ++QDQA L AV VF LED A Sbjct: 487 QAAAAARSMQDQAASLTDAVSVFRLEDDA 515 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.125 0.320 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 850,710 Number of extensions: 30786 Number of successful extensions: 588 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 259 Number of HSP's successfully gapped: 71 Length of query: 363 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 277 Effective length of database: 1,212,660 Effective search space: 335906820 Effective search space used: 335906820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)