BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0245.0033.p (499 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 657 0.0 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 285 4e-78 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 239 4e-64 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 238 8e-64 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 232 5e-62 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 657 bits (1696), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/470 (76%), Positives = 404/470 (85%), Gaps = 9/470 (1%) Query: 15 KLLKPLQGFRTSSALLAAMLVLAGCSLAPTYERPAVNTSVAYKEAADA----KAASDWKT 70 +LL+P+ R SALLAA+LVLAGCS+APTYERP+V+T A+KEAA A + S WKT Sbjct: 10 RLLRPV---RIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKT 66 Query: 71 AQPAEDMARGEWWKIFNDEGLNALEAQALEANQNLKAAAARLSQSRALRQDARSGLFPQV 130 AQPAED+ARGEWWKIF DE LNALE A +ANQ+LKAAAARL Q+RAL +DAR+G FPQV Sbjct: 67 AQPAEDIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQV 126 Query: 131 DAGFGATRQRQSPASQGLPPNANTTPTTLYRAQLGVSYEADLFGRVASTVDAATADAQRS 190 DAG GATRQR SPASQGLP +A+T P TLYRAQ+ VSYE DLFGRVASTVDAATADAQRS Sbjct: 127 DAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRS 186 Query: 191 EALFHSVRLALQADVAQQYFLLRELDAEQELYTGTVDLRMQSLKLVQRRFDEGDISEVDV 250 EALFHSV LALQADVAQQYFL+RELDA LY GTVDLR +SL+LVQRRFDEGDISEVDV Sbjct: 187 EALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDV 246 Query: 251 ARAKSELASAQSESFGIARQRANAEHALAILLGKTPAEFDFKPQPLQRLSIAIPAGLPSD 310 ARA++ELASAQSE+ GIARQRA AEHALAILLG+TPAEF PQPL RL++ +P GLPS Sbjct: 247 ARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPPGLPSA 306 Query: 311 LLERRPDXRPDGRLDDLAAANARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTFL 370 LLERRPD +AAANARVGVA+SA+FP L+LTGGLGYESA+LGDLFNWSSRTFL Sbjct: 307 LLERRPDI--AAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFL 364 Query: 371 LGPLVGTALTLPIFDGGRREAGVNRARAAYEEDVANYRQTVLTAFGEVEDNLASLRILGD 430 LGPLVGTAL++P+FDGGRR+AGV+RARA+YEE VA+YR TVL AF EVEDNLA+LRIL Sbjct: 365 LGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQ 424 Query: 431 QTRAQDDAVSASNHAAKLSHVQYREGSVSYFNVIDADRTVLQQQRAAVQL 480 Q RAQD+AV+AS AAKLSH QYREG+VSYF+VIDADRTVLQQQRA+V L Sbjct: 425 QNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTL 474 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 285 bits (730), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 194/481 (40%), Positives = 277/481 (57%), Gaps = 23/481 (4%) Query: 17 LKPLQGFRTSSALLAAMLVLAGCSLAPTYERPAVNTSVA-YKEAADAKAASDWKTAQPAE 75 LKPL A+L A L LA CS+ P Y RP ++T A +KE +WK AQP + Sbjct: 9 LKPL------LAMLVASLALAACSVGPDYVRPQMDTGPAIFKEG------QNWKPAQPRD 56 Query: 76 DMARGEWWKIFNDEGLNALEAQALEANQNLKAAAARLSQSRALRQDARSGLFPQVDAGFG 135 +A G+WW++F D LNAL AQ +NQNL A A + AL Q +RS P + A G Sbjct: 57 LVADGKWWEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAG 116 Query: 136 ATRQRQSPASQGLPPNANTTPTTL----YRAQLGVSYEADLFGRVASTVDAATADAQRSE 191 A+R A A+ + Y L ++EAD++G+V V+++ A AQ S Sbjct: 117 ASRGSSGSAGASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASA 176 Query: 192 ALFHSVRLALQADVAQQYFLLRELDAEQELYTGTVDLRMQSLKLVQRRFDEGDISEVDVA 251 A + RL+ QA VAQ Y LR LDA+Q+L TV +SL++ Q ++ G +++ DV Sbjct: 177 ADLAAARLSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVI 236 Query: 252 RAKSELASAQSESFGIARQRANAEHALAILLGKTPAEFDFKPQPLQRLSIAIPAGLPSDL 311 +A+++L SAQ+ + RA EHA+A+L+GKTPA+F PL ++ +P G+PS L Sbjct: 237 QAQTQLKSAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSML 296 Query: 312 LERRPDXRPDGRLDDLAAANARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTFLL 371 LERRPD R +A+ANA++GVA++A+FP L L+ GY+S+ D F R + L Sbjct: 297 LERRPDVAAAER--RVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSL 354 Query: 372 GPLVGTALTLPIFDGGRREAGVNRARAAYEEDVANYRQTVLTAFGEVEDNLASLRILGDQ 431 GP L IFDGG R A ++A A Y+ VA Y+QTVLTAF EVEDNLA+LRIL + Sbjct: 355 GP----QLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQE 410 Query: 432 TRAQDDAVSASNHAAKLSHVQYREGSVSYFNVIDADRTVLQQQRAAVQLPHAELVGFFIL 491 Q +A+ A+ A L + QY+ G+VSY NVI A T L + +++ + + + +L Sbjct: 411 AAVQGEALQAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLL 470 Query: 492 C 492 Sbjct: 471 A 471 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 239 bits (610), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 173/458 (37%), Positives = 245/458 (53%), Gaps = 17/458 (3%) Query: 38 GCSLAPTYERPAVNTSVAYKEAADAKAASDWKTAQPAEDMARGEWWKIFNDEGLNALEAQ 97 GC+ P Y RP V+ A+ A W++ QPA+ RG WW+ F D LNAL+ Q Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAP------WRSMQPADATPRGPWWERFGDAQLNALQQQ 71 Query: 98 ALEANQNLKAAAARLSQSRALRQDARSGLFPQVDAGFGATRQRQS---PASQGLPPNANT 154 AL NQ L A ARL+Q+RA + PQV+ A R R S P + PN +T Sbjct: 72 ALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSPNFST 131 Query: 155 TPTTLYRAQLGVSYEADLFGRVASTVDAATADAQRSEALFHSVRLALQADVAQQYFLLRE 214 + VSYE D FGRV +V+AA A A++S A + RL L A++A YF LRE Sbjct: 132 VQND-FALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRE 190 Query: 215 LDAEQELYTGTVDLRMQSLKLVQRRFDEGDISEVDVARAKSELASAQSESFGIARQRANA 274 LD E ++ + L+ Q+L+L R D G S +D+A+ ++ L S ++ + RQRA Sbjct: 191 LDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQY 250 Query: 275 EHALAILLGKTPAEFDFKPQPLQRLSIAIPAGLPSDLLERRPDXRPDGRLDDLAAANARV 334 EHA+A L G F P AIP G+PSD+LERRPD +AAANA++ Sbjct: 251 EHAIATLTGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDI--AAAERAMAAANAQI 308 Query: 335 GVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTFLLGPLVGTALTLPIFDGGRREAGVN 394 GVA +A++P L G +S LFN S + L G + + +FDGGR AGV+ Sbjct: 309 GVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSL----GVSASQSLFDGGRLRAGVD 364 Query: 395 RARAAYEEDVANYRQTVLTAFGEVEDNLASLRILGDQTRAQDDAVSASNHAAKLSHVQYR 454 ++A YE VA+YR+TVLTA EVED + L L A++++ +++ +Y Sbjct: 365 FSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYE 424 Query: 455 EGSVSYFNVIDADRTVLQQQRAAVQLPHAE-LVGFFIL 491 G+ Y +VI A +++L +R A QL LV F++ Sbjct: 425 GGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLI 462 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 238 bits (607), Expect = 8e-64, Method: Compositional matrix adjust. Identities = 185/487 (37%), Positives = 266/487 (54%), Gaps = 36/487 (7%) Query: 23 FRTSSALLAAML--VLAGCSLAPTYERPAVNTSVAYKEAADAKAASDWKTAQPAEDMARG 80 FR + + A +L +L GC++ P Y RPA++ AYKE WK A P + Sbjct: 29 FRATKGMTALVLAALLGGCAVGPDYARPALDLPQAYKETGP------WKLAAPRQIDDHQ 82 Query: 81 EWWKIFNDEGLNALEAQALEANQNLKAAAARLSQSRALRQDARSGLFPQVDAGFGATRQR 140 WW+ + D LNAL QA ANQ ++ AAA+ Q++A + AR+ L+P + A GA+R + Sbjct: 83 PWWEAYGDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQ 142 Query: 141 QSPASQGLPPNANTTPTTLYRAQLGVSYEADLFGRVASTVDAATADAQRSEALFHSVRLA 200 + + G+ N+ Y L S+EADL+GR+ +A A+AQ S A + RL+ Sbjct: 143 TN--TNGVQRLGNS-----YSVGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLS 195 Query: 201 LQADVAQQYFLLRELDAEQELYTGTVDLRMQSLKLVQRRFDEGDISEVDVARAKSELASA 260 +QA +AQ Y LR D +++LY TV +SL+L +++ G DVA+A+++L +A Sbjct: 196 IQATLAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAA 255 Query: 261 QSESFGIARQRANAEHALAILLGKTPAEFDFKPQP---------------LQRLSIAIPA 305 Q++ + R EHA+A+L+GK PA+F P LQ IP Sbjct: 256 QAQLIDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANAARLQGSLPQIPV 315 Query: 306 GLPSDLLERRPDXRPDGRLDDLAAANARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWS 365 GLPS+LLERRPD AAANA +GVAR+A+FP L L+ GY S +LFN Sbjct: 316 GLPSELLERRPDI--ANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTP 373 Query: 366 SRTFLLGPLVGTALTLPIFDGGRREAGVNRARAAYEEDVANYRQTVLTAFGEVEDNLASL 425 SR + L G+AL +FDGG R A + A AAY+ VA Y+QTVL EVED L++L Sbjct: 374 SRVWSL----GSALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTL 429 Query: 426 RILGDQTRAQDDAVSASNHAAKLSHVQYREGSVSYFNVIDADRTVLQQQRAAVQLPHAEL 485 R+L ++ Q AV ++ A +L+ QY G+V+Y +V+ L QR AV L +L Sbjct: 430 RVLDQESTVQAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQL 489 Query: 486 VGFFILC 492 V L Sbjct: 490 VASVALI 496 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 232 bits (591), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 165/449 (36%), Positives = 237/449 (52%), Gaps = 24/449 (5%) Query: 35 VLAGCSLAPTYERPAVNTSVAYKEAADAKAASDWKTAQPAEDMARGEWWKIFNDEGLNAL 94 VL GCSLAPTYERP +Y ADA ++ + W + F D+ L L Sbjct: 24 VLGGCSLAPTYERPQAPVESSYP--ADAAGRAELQAVNLG-------WRQFFPDQRLQQL 74 Query: 95 EAQALEANQNLKAAAARLSQSRALRQDARSGLFPQVDAGFGATRQRQSPASQGLPPNANT 154 A ALE N++L+ A + ++RA Q AR+ L P ++ A R R + ASQ L Sbjct: 75 IAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR-TAASQSL--TGQP 131 Query: 155 TPTTLYRAQLGV-SYEADLFGRVASTVDAATADAQRSEALFHSVRLALQADVAQQYFLLR 213 T + Y+ L V +YE D FGRV + DAA A +E S +++L A VAQ Y R Sbjct: 132 TISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAER 191 Query: 214 ELDAEQELYTGTVDLRMQSLKLVQRRFDEGDISEVDVARAKSELASAQSESFGIARQRAN 273 + +L T+ R +L L Q+R D G + +D ++ + SA+ + RQRA Sbjct: 192 SYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQ 251 Query: 274 AEHALAILLGKTPAEFDFKPQPL---QRLSIAIPAGLPSDLLERRPDXRPDGRLDDLAAA 330 AE+AL +L+GK E P L +++ IPAGLPSDLL RPD R + L AA Sbjct: 252 AENALTLLVGKKIGE--LPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQ--SLLAA 307 Query: 331 NARVGVARSAWFPRLDLTGGLGYESAKLGDLFNWSSRTFLLGPLVGTALTLPIFDGGRRE 390 NA +G AR+A+FPR+ LTG G S+ LG LF S + GP +L+LPIFD GR Sbjct: 308 NANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGP----SLSLPIFDFGRNS 363 Query: 391 AGVNRARAAYEEDVANYRQTVLTAFGEVEDNLASLRILGDQTRAQDDAVSASNHAAKLSH 450 A ++ A + V Y + + TAF EV D L + L +Q +AQ+ ++A KL+ Sbjct: 364 ANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTD 423 Query: 451 VQYREGSVSYFNVIDADRTVLQQQRAAVQ 479 ++++ G S + +DA R + Q+A +Q Sbjct: 424 LRFKNGIASSLDQLDAQRDLFSAQQALIQ 452 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,131,241 Number of extensions: 39188 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 18 Length of query: 499 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 410 Effective length of database: 1,198,455 Effective search space: 491366550 Effective search space used: 491366550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)