BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0245.0025.p (235 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 168 3e-43 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 168 3e-43 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 144 5e-36 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 125 4e-30 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 91 9e-20 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 168 bits (426), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 92/222 (41%), Positives = 144/222 (64%), Gaps = 9/222 (4%) Query: 1 VGPVGLYIRSQIDETPEFKKIAADAAVAKAEKSPLMLVLVRDRVKLLLAIGVVAGATAFN 60 + P G+YIR +DE PEFK+ ++ +PL +++LL+ G V AT Sbjct: 203 IAPAGIYIRRHLDEAPEFKE------SSEKTNAPLRDTFAHQKMRLLIGAGSVIMATVSV 256 Query: 61 YVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRF-RVLTIAMLS 119 Y+ LY+PTYA+KQL +PA SSF + GL++ + +P+ G LSD+ GR ++T + L Sbjct: 257 YL-SLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALY 315 Query: 120 IGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTQTRGTGLALSY 179 I LT YP+F+ L P L+ +Q ++GVL+ +PA+L+DIFP TRGTG++L+Y Sbjct: 316 IVLT-YPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAY 374 Query: 180 NFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVA 221 N +VT+FGGF+ LI+TW+I T +K++ S++V+ A++SL+A Sbjct: 375 NIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIA 416 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 168 bits (426), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 97/235 (41%), Positives = 141/235 (60%), Gaps = 15/235 (6%) Query: 1 VGPVGLYIRSQIDETPEF--KKIAADAAVAKAEKSPLMLVLVRDRVKLLLAIGVVAGATA 58 +GPVG YIR ++ETP F +K D SPL V+ + + +V T Sbjct: 206 IGPVGYYIRHHLEETPTFLEEKDKTD--------SPLKEVIRNFPRETSASFSMVILWTV 257 Query: 59 FNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRFRVLTIAML 118 YV YMPTY++K L +P T+ FI ++ GL +M+ AP+ G L+DR GR +L+ + Sbjct: 258 CTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLLSGSAA 317 Query: 119 SIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTQTRGTGLALS 178 I + +YP+F +N+ P+++TL+ Q + G LIAA GPI A S++FP + TGL+++ Sbjct: 318 LILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLSVA 377 Query: 179 YNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVAVIVLGRRMGRNP 233 YN +VT+FGGF+ +TW+IAAT + MAP+FYVM A IS V G R R P Sbjct: 378 YNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFV-----GTRFVREP 427 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 144 bits (364), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 85/223 (38%), Positives = 136/223 (60%), Gaps = 7/223 (3%) Query: 1 VGPVGLYIRSQIDETPEFKKIAADAAVAKAEKSPLMLV-LVRDRVK-LLLAIGVVAGATA 58 +GPVGL+IR + ET +F + A K +P+ L+ +V+ ++ +L+++G V T Sbjct: 194 IGPVGLWIRRHMHETDDFLESAN-----KPNAAPIKLLDVVKTNLRGVLVSMGQVINGTV 248 Query: 59 FNYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRFRVLTIAML 118 YV + MPT+A KQL +P +F+ +I +L P G LSDR GR VL + L Sbjct: 249 AFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLYSSL 308 Query: 119 SIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTQTRGTGLALS 178 + L YPLF+ ++ P++ L+ +Q ++ ++I A GP+P LS+ FPT+ R TGLA++ Sbjct: 309 AFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGLAVA 368 Query: 179 YNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVA 221 YN + LFGGF+P IVTW+ + + +AP++YV+ A I + A Sbjct: 369 YNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSA 411 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 125 bits (313), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 77/209 (36%), Positives = 119/209 (56%), Gaps = 14/209 (6%) Query: 1 VGPVGLYIRSQIDETPEFKKIAADAAVAKAEKSPLMLVLVRDRV-KLLLAIGVVAGATAF 59 +GPVG YIR + ETPEF++ K E L V+ ++L+ I ++ T Sbjct: 200 IGPVGWYIRRVLPETPEFER------SKKKESVDSFLEGVKANFGRILIGIAIMIFWTVA 253 Query: 60 NYVHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGRFRVLTIAMLS 119 V Y TYA+++LH+P S+++G + +GL L+ P+ G L+DR G ++ + Sbjct: 254 TQVSN-YFTTYAVRELHMPVASTYLGQISSGLTLLAICPLVGMLADRIGPYKPMAFGAAL 312 Query: 120 IGLTSYPLFMLLNTWPTVTTLIAVQGIVGV---LIAACLGPIPAMLSDIFPTQTRGTGLA 176 G+ +YPLF +L PT+ TL+ VQ + V L AAC P ++SDIFPT+ R TG+ Sbjct: 313 AGVLAYPLFKMLVATPTLPTLLLVQIAISVTLGLYAAC---APLVMSDIFPTRYRATGIG 369 Query: 177 LSYNFSVTLFGGFSPLIVTWMIAATENKM 205 SY V +FGG +P+IVT +I + +K+ Sbjct: 370 TSYALGVMIFGGLTPMIVTTLIQISGDKL 398 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 90.9 bits (224), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 69/233 (29%), Positives = 113/233 (48%), Gaps = 19/233 (8%) Query: 3 PVGLYIRSQIDETPEFKKIAADAAVAKAEKSPLMLVLVRDRVKLLLA-IGVVAGATAFNY 61 PV IR + ET EF + + + +S + + KL++A + +V+ T Y Sbjct: 199 PVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMI------ENWKLVIAGMMLVSMTTVSFY 252 Query: 62 VHKLYMPTYALKQLHIPATSSFIGALITGLVLMLTAPVFGTLSDRYGR------FRVLTI 115 + +Y PT+ L + +S I L GL + P+ G LSDR GR F VLTI Sbjct: 253 LITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTFTVLTI 312 Query: 116 AMLSIGLTSYPLFMLLNTWPTVTTLIAVQGIVGVLIAACLGPIPAMLSDIFPTQTRGTGL 175 LT+YP L + ++ V+ + L A+ G + L+++ P R G Sbjct: 313 ------LTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAGF 366 Query: 176 ALSYNFSVTLFGGFSPLIVTWMIAATENKMAPSFYVMATALISLVAVIVLGRR 228 +L+Y+ + +FGGF+P I T +I T +K AP ++ A+ L+A ++L R Sbjct: 367 SLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRN 419 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 624,385 Number of extensions: 23607 Number of successful extensions: 184 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 182 Number of HSP's successfully gapped: 10 Length of query: 235 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 153 Effective length of database: 1,231,600 Effective search space: 188434800 Effective search space used: 188434800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)