BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0245.0024.p (193 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 161 3e-41 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 157 6e-40 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 155 2e-39 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 136 1e-33 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 133 9e-33 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 161 bits (408), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 86/159 (54%), Positives = 113/159 (71%), Gaps = 10/159 (6%) Query: 12 SATASPAAKRKNPW--KEICAASIGNALEFYDLLIYGYFAVVIGKLFFPAQDETTSLLLS 69 S TA+P A +P + I ++SIGNALE++D+LIYG FAVVI K FFP D++ SLLL+ Sbjct: 3 SNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLT 62 Query: 70 VGTFGISFVIRPLGAIVLGSYADRAGRKASLTASILIMMVGTAMIAFAPTYAQIGIASPL 129 TFG+SF +RPLGA+VLG+Y+DRAGRKA+L SI++M VGTAMIAF P+YA IG+ +P Sbjct: 63 FATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPA 122 Query: 130 IIIVARLLQGFSTGGEKGG--------ARRRRQRFAQLQ 160 I + +++QGFS GGE G A RR F+ Q Sbjct: 123 GIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQ 161 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 157 bits (397), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 79/151 (52%), Positives = 105/151 (69%), Gaps = 2/151 (1%) Query: 7 SSSPLSATASPAAKRKNPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPAQDETTSL 66 S+ P S + +++ + I A IGN LE++D +Y +FAV+I KLFFP ++ TSL Sbjct: 4 STLPADGIVSVSNQQRR--RAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSL 61 Query: 67 LLSVGTFGISFVIRPLGAIVLGSYADRAGRKASLTASILIMMVGTAMIAFAPTYAQIGIA 126 LL+V TFG+ F +RP+G I+LG YADR GRKA+L+ +IL+M VGT MI APTY IG+ Sbjct: 62 LLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLF 121 Query: 127 SPLIIIVARLLQGFSTGGEKGGARRRRQRFA 157 +PLII+VARLLQGFS GGE G A +A Sbjct: 122 APLIIVVARLLQGFSAGGEMGSATAFLTEYA 152 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 155 bits (393), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 69/123 (56%), Positives = 100/123 (81%) Query: 23 NPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPAQDETTSLLLSVGTFGISFVIRPL 82 N +++ AA +GNALE+YD ++YG+F+ +I +LFFPA +E TSLL+++ TFGI F +RP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 83 GAIVLGSYADRAGRKASLTASILIMMVGTAMIAFAPTYAQIGIASPLIIIVARLLQGFST 142 G ++LG YADR GRKA++ I++M + A+IAF PTYA IGIA+P++I+VAR+LQGF+T Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 143 GGE 145 GGE Sbjct: 126 GGE 128 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 136 bits (342), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 63/136 (46%), Positives = 90/136 (66%) Query: 14 TASPAAKRKNPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPAQDETTSLLLSVGTF 73 T + + + + + GN LE +D +YG++A I K FFP+ +E SL+L+ GTF Sbjct: 2 TTTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTF 61 Query: 74 GISFVIRPLGAIVLGSYADRAGRKASLTASILIMMVGTAMIAFAPTYAQIGIASPLIIIV 133 G F++RPLGAI+LGSY DR GR+ L ++ IM +GT +IAF P YA IG+A+P++++ Sbjct: 62 GAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLT 121 Query: 134 ARLLQGFSTGGEKGGA 149 RLLQGFS G E GG Sbjct: 122 GRLLQGFSAGVELGGV 137 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 133 bits (335), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 66/148 (44%), Positives = 94/148 (63%), Gaps = 9/148 (6%) Query: 22 KNPWKEICAASIGNALEFYDLLIYGYFAVVIGKLFFPAQDETTSLLLSVGTFGISFVIRP 81 K + + AA++GN LE YD +I+G FAV I K FFP+ DE +L+ + TF FV+RP Sbjct: 11 KTQRRAVIAATLGNVLEIYDFIIFGIFAVAISKTFFPSGDEYAALMFTFMTFAAGFVVRP 70 Query: 82 LGAIVLGSYADRAGRKASLTASILIMMVGTAMIAFAPTYAQIGIASPLIIIVARLLQGFS 141 +GA+VLG YAD+ GRK +L+ ++++M +GT + A P+Y IGIA+ +I++ RLLQGFS Sbjct: 71 IGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAATVILVFGRLLQGFS 130 Query: 142 TGGEKG---------GARRRRQRFAQLQ 160 GGE G + R R F Q Sbjct: 131 AGGEIGSNVAMLVENASSRNRALFCSFQ 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 414,247 Number of extensions: 14507 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 11 Length of query: 193 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 113 Effective length of database: 1,241,070 Effective search space: 140240910 Effective search space used: 140240910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)