BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0245.0023.p (304 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313153|ref|YP_003777245.1| transcription regulator protein... 173 1e-44 gi|300313854|ref|YP_003777946.1| LysR family transcription regul... 149 2e-37 gi|300310792|ref|YP_003774884.1| LysR family transcription regul... 59 4e-10 >gi|300313153|ref|YP_003777245.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 331 Score = 173 bits (439), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 106/297 (35%), Positives = 155/297 (52%), Gaps = 8/297 (2%) Query: 5 WLEDFIELARTRSFSRAAENRFVTHPAFGRRIKALEQWVGAELVVRT-HPVSLTAAGQLF 63 WLEDF+ LA TRSFSR+AE R VT PAF RRI++LE W+G +L+ RT +P LTAAG++F Sbjct: 5 WLEDFVSLAETRSFSRSAELRHVTQPAFSRRIQSLEAWLGTDLIDRTSYPTRLTAAGEVF 64 Query: 64 LDAATHSTDVLYAARAQLQDGSGPAEKTLRVATGRTLARTFFPDWYENINQRFGPFPVSV 123 + A + RA L+ A+ T+ A TL+ T+ P W + FG + Sbjct: 65 YEQAVAMLGQINNTRALLRGKRPTAQTTIDFAVPHTLSLTYMPKWLSALESGFGAINTRL 124 Query: 124 STGGAQEVILRLGAGDADLLVIYSSPTTRLLIDPMRYESLTLAREILLPVVAADARGRPR 183 + ++ + G DLL+ Y P + +D RY+ LT+ E L P D G+P Sbjct: 125 IALNVHDAVMTIVEGGCDLLLCYHHPRQPVQLDSSRYDMLTMGTETLRPYSRCDRNGKPD 184 Query: 184 FKLAPANAATPTPWLGFSQSLTLRGVLAKHLAGLPKKVSLRMVYEADSYESIHEMARRGV 243 + + P +A P P+L ++ + L ++ +A + + L YE D ES+ MA G Sbjct: 185 Y-VFPGSAKAPVPFLSYTSNAYLGRMVELMMADTKRPLHLDKHYETDMSESLKMMALEGH 243 Query: 244 GVAWLPQRLVQEDVNERRLLVIGEP--EMRVNFDISLYRVRGN----DDALVNAIWD 294 GVA+LP+ V +V R+L P E V +I LYR R ALV +W+ Sbjct: 244 GVAFLPESSVVREVRNRQLARTDVPSGEWEVEMEIRLYRERPTAQRAGKALVARLWE 300 >gi|300313854|ref|YP_003777946.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 310 Score = 149 bits (377), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 99/309 (32%), Positives = 156/309 (50%), Gaps = 11/309 (3%) Query: 1 VHLIWLEDFIELARTRSFSRAAENRFVTHPAFGRRIKALEQWVGAELVVRT-HPVSLTAA 59 + + W+EDF+ + T +FSR+A+ R VT PAFGRRI++LE W+GAEL R+ +P +LT A Sbjct: 1 MEIKWVEDFLSVVETLNFSRSAKLRNVTQPAFGRRIRSLETWLGAELFDRSSYPCTLTQA 60 Query: 60 GQLFLDAATHSTDVLYAARAQLQDGSGPAEKTLRVATGRTLARTFFPDWYENINQRFGPF 119 G+ F+ A + AR L+ A+ +R+A TL T FP I + G Sbjct: 61 GESFVPIAKEMLNQSMQARLVLRGQLAGAQSAVRLAMPHTLLLTVFPKLLSEIEKSIGAI 120 Query: 120 PVSVSTGGAQEVILRLGAGDADLLVIYSSPTTRLLIDPMRYESLTLAREILLPVVAADAR 179 +V G + ++ L + DLL+ Y + +D RY+ L+L +E L P V Sbjct: 121 STTVMAGNVHDAVMALVERNCDLLLCYDHRQQGIALDTERYDVLSLGKEPLRPYVKTLPN 180 Query: 180 GRPRFKLAPANAATPTPWLGFSQSLTLRGVLAKHLAGLPKKVSLRMVYEADSYESIHEMA 239 G P++ L P P P+L +S +L ++ K L P++V L +E D E + MA Sbjct: 181 GLPKYVL-PGAPNDPQPFLSYSPYSSLSRIVEKALHSSPRQVHLFRRFETDLAEGLKSMA 239 Query: 240 RRGVGVAWLPQRLVQEDVNERRL-LVIGEPEMRV--------NFDISLYRVRGNDDALVN 290 G G+AWLP V ++ E +L L I + DI +YR + ++ Sbjct: 240 VEGHGIAWLPASAVAREIAEGKLTLAIAPTDSEALEQGLWCDEMDIRVYRRLDGANPDID 299 Query: 291 AIWDGVASD 299 IW+ + ++ Sbjct: 300 RIWNYLKAE 308 >gi|300310792|ref|YP_003774884.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 46/142 (32%), Positives = 67/142 (47%), Gaps = 1/142 (0%) Query: 6 LEDFIELARTRSFSRAAENRFVTHPAFGRRIKALEQWVGAELVVR-THPVSLTAAGQLFL 64 L+ FI +AR SF AAE F+T PA RRI+ LEQ + L R T VSLT AG+LFL Sbjct: 10 LQAFIAVARQSSFRAAAEGLFITQPALSRRIENLEQALQERLFDRTTRRVSLTPAGELFL 69 Query: 65 DAATHSTDVLYAARAQLQDGSGPAEKTLRVATGRTLARTFFPDWYENINQRFGPFPVSVS 124 A + L A ++ + + VA ++A P + + V + Sbjct: 70 VHAQAVMEELELAVKSVEQRMAQRREHVTVACVPSVANNLLPQVLKRYARSHANVRVRII 129 Query: 125 TGGAQEVILRLGAGDADLLVIY 146 A +V+ + G+AD V + Sbjct: 130 DESAADVLESVRKGEADFGVNF 151 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,014,123 Number of extensions: 43696 Number of successful extensions: 236 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 4 Length of query: 304 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 219 Effective length of database: 1,217,395 Effective search space: 266609505 Effective search space used: 266609505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)