BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0245.0022.p (543 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protei... 865 0.0 gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolas... 165 9e-42 gi|300310565|ref|YP_003774657.1| response regulator containing a... 74 3e-14 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 71 2e-13 gi|300313980|ref|YP_003778072.1| two component response regulato... 70 4e-13 >gi|300309652|ref|YP_003773744.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 552 Score = 865 bits (2236), Expect = 0.0, Method: Compositional matrix adjust. Identities = 412/539 (76%), Positives = 474/539 (87%), Gaps = 1/539 (0%) Query: 4 QHLDISHRLEKLTELSVELSTNRNIPLLLERILQTARSITLADGGTLYRTVEDEDSLAFY 63 +H +I++RLE+LTEL EL ++R+IPLLLERIL TA+ IT ADGGTLYR E DSL FY Sbjct: 12 RHRNIAYRLERLTELCRELGSSRDIPLLLERILLTAKDITQADGGTLYRINEGGDSLRFY 71 Query: 64 ISINDTLGMHQGGSSAQKISIPNIQLTSPDGSKNLSAVAAYAANTRKSVNIPDVYQ-AEG 122 IS+NDTL MHQGGSS + ISIP+I L PDG NLSAVAAYAANTRKSVNI DVYQ + G Sbjct: 72 ISVNDTLKMHQGGSSGKAISIPDIALRLPDGQPNLSAVAAYAANTRKSVNIADVYQVSSG 131 Query: 123 FNFSGMRMFDEKFGYHSQSFLTVPMQDHEGELVGVLQLVNAIDPATGKPHPFSETDQYFI 182 FNF+GMR+FDEK+GY S+SFLTVPM+DHEGELVGVLQL+NAIDPATG P PFSETDQ+FI Sbjct: 132 FNFNGMRLFDEKYGYQSKSFLTVPMEDHEGELVGVLQLINAIDPATGAPLPFSETDQHFI 191 Query: 183 EALASQAAIAITNQQLIYRLENLFEHFIKLINMGIDEKSPHTGRHCEHVPELAMMLAEAS 242 EALASQAA+A+TNQQLI RLE LFE I+LIN+GI EKSPHTGRHCE VPELAMMLAEA+ Sbjct: 192 EALASQAAMAMTNQQLIQRLEQLFESLIRLINIGIGEKSPHTGRHCEQVPELAMMLAEAA 251 Query: 243 HRTTEGPLADFQMTERDRRELWVAGLLHDCGKITTPDHIVEKSTKLQTIYDRIHLVDARY 302 H TEGPLADF M+E DRRELW+A LLHDCGKI+TP HIVEK+TKL+TI+DRIHLV+ R Sbjct: 252 HNATEGPLADFHMSEADRRELWLAALLHDCGKISTPTHIVEKATKLETIFDRIHLVETRI 311 Query: 303 EVLKRDAEIRVLKEKCALGAALTAQKSAELDAALAAECEALDAERNFLRKSNVGSEGMKP 362 EVLKRDAEIRVL+EK ALGAAL AQ++AELDA LAAE LD ER FLRK NVG+EGM+P Sbjct: 312 EVLKRDAEIRVLQEKLALGAALDAQRAAELDAGLAAELAQLDLERAFLRKINVGTEGMRP 371 Query: 363 DDQQRVLEIARRQWRGPDGTMQDLLSTDEAENLRIRAGTLNDAERQVINNHIVVTIKMLE 422 +DQQRVL+IA+R WRGPDG ++ LL +E+ NLRIRAGTLNDAERQ+INNHIV+TIKMLE Sbjct: 372 EDQQRVLDIAQRSWRGPDGQLRPLLDEEESANLRIRAGTLNDAERQIINNHIVITIKMLE 431 Query: 423 SLPWPKQLRNVTEYAGGHHERMDGKGYPKGLTREQMSPQARMMAIADIFEALTAADRPYK 482 SLPWPKQL VTE+AGGHHERMDGKGYPKGLTREQMS QAR+MA+ADIFEA+TAADRPYK Sbjct: 432 SLPWPKQLARVTEFAGGHHERMDGKGYPKGLTREQMSVQARIMAVADIFEAVTAADRPYK 491 Query: 483 KGNTLSEALEILGGFRLRNHIDPDLFDVFVRSKVYQQYADKFMPASQIDVVDHSKIPGF 541 +GNTL+EA+EIL GF+ R+HIDPDLFD+FVR ++Y++YA+KFM SQID VD S+IPGF Sbjct: 492 RGNTLNEAVEILAGFKARDHIDPDLFDLFVRERIYEKYAEKFMHPSQIDEVDLSRIPGF 550 >gi|300309839|ref|YP_003773931.1| metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 975 Score = 165 bits (417), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 97/230 (42%), Positives = 136/230 (59%), Gaps = 12/230 (5%) Query: 147 MQDHEGELVGVLQLVNAIDPATGKPHPFSETDQYFIEALASQAAIAITNQQLIYRLENLF 206 ++ G L G+L LV+A D A G P + F L+ A+AI +QLI + L Sbjct: 523 LRGRNGSLEGLLVLVHAHDSAHGDPRFLA-----FARRLSGMLAVAIETRQLIAAQKALL 577 Query: 207 EHFIKLINMGIDEKSPHTGRHCEHVPELAMMLAEASHRTTEGPLADFQMTERDRRELWVA 266 E I+L+ ID KSP+TG HCE VP LA+ML E + EGP A F + E +R +A Sbjct: 578 ESLIRLMADAIDAKSPYTGGHCERVPHLAIMLVERLAQEREGPYAGFSLNEDERYAFRLA 637 Query: 267 GLLHDCGKITTPDHIVEKSTKLQTIYDRIHLVDARYEVLKRDAEIRVLKEKCALGAALTA 326 LLHDCGK+T+P+HIV+K+TKL+ I++RIH V R+EVL RDAE+ +L + L AL+ Sbjct: 638 ALLHDCGKVTSPEHIVDKATKLEVIHNRIHEVRMRFEVLWRDAELALLHQ---LPQALSE 694 Query: 327 QKSAELDAALAAECEALDAERNFLRKSNVGSEGMKPDDQQRVLEIARRQW 376 Q++ AL A+ L + F+ + NVGSE M + R+ IA + W Sbjct: 695 QQA----QALEAQRARLREDFAFVAQCNVGSEAMSEEAIARLARIATQTW 740 Score = 158 bits (399), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 75/148 (50%), Positives = 101/148 (68%) Query: 391 EAENLRIRAGTLNDAERQVINNHIVVTIKMLESLPWPKQLRNVTEYAGGHHERMDGKGYP 450 E NL IR GTL +R INNHIV T+ ML+SLPWP+ L++V + A HHE+MDG GYP Sbjct: 819 ELHNLGIRRGTLTPEDRFAINNHIVQTLVMLKSLPWPESLKSVPDTAANHHEKMDGTGYP 878 Query: 451 KGLTREQMSPQARMMAIADIFEALTAADRPYKKGNTLSEALEILGGFRLRNHIDPDLFDV 510 + L ++ Q R+MA+AD+FEALTAADRPYK TLSE+L I+ H+D +LF Sbjct: 879 RRLKASELPLQDRVMALADVFEALTAADRPYKPAKTLSESLRIMANMCRERHLDTELFRY 938 Query: 511 FVRSKVYQQYADKFMPASQIDVVDHSKI 538 F+RS++++ YA + + QID VD +I Sbjct: 939 FLRSRLWEVYAGELLKEGQIDAVDIVEI 966 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 44/129 (34%), Positives = 73/129 (56%), Gaps = 3/129 (2%) Query: 383 MQDLLSTDEAENLRIRAGTLNDAERQVINNHIVVTIKMLESLPWPKQLRNVTEYAGGHHE 442 M D+ +++ ++ G L++ E +V+ H + +L + P P L+ ++ A HHE Sbjct: 205 MHDIGKMGIPDHILLKPGRLDEEELKVMRMHARIGADILVASPSPL-LQTASQIALTHHE 263 Query: 443 RMDGKGYPKGLTREQMSPQARMMAIADIFEALTAADRPYKKGNTLSEALEILGGFRLRNH 502 + DG GYP GL EQ+ R++A+AD+F+ALT++ RPYK G +L A + L + H Sbjct: 264 KWDGSGYPNGLKGEQIPLYGRIVAVADVFDALTSS-RPYKPGWSLERASQYLRE-QSGAH 321 Query: 503 IDPDLFDVF 511 DP + F Sbjct: 322 FDPRCVEAF 330 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 71.2 bits (173), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 50/149 (33%), Positives = 75/149 (50%), Gaps = 14/149 (9%) Query: 370 EIARRQWRGPDGTMQDLLSTDEAENLRIRAGTLNDAERQVINNHIVVTIKMLESLPWPKQ 429 E A R+ G G + D+ L + G L+D E I H + K L P Sbjct: 181 ETACRE-AGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDP---- 235 Query: 430 LRNVTEYAGG----HHERMDGKGYPKGLTREQMSPQARMMAIADIFEALTAADRPYKKGN 485 NV +YA HHE++DG GYP+GL ++ +RM AI D+++ALT +DRPYK+ Sbjct: 236 --NVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALT-SDRPYKRAW 292 Query: 486 TLSEALEILGGFRLRNHIDPDLFDVFVRS 514 +EA+ + + H D +F FV++ Sbjct: 293 DPAEAISAMASW--TGHFDRAMFLAFVKT 319 >gi|300313980|ref|YP_003778072.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 199 Score = 70.1 bits (170), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 40/123 (32%), Positives = 69/123 (56%), Gaps = 7/123 (5%) Query: 395 LRIRAGTLNDAERQVINNHIVVTIKMLESLPWPKQLRNVTEYAGGHHERMDGKGYPKGLT 454 L + G ++ E V+ H+ ++L + +P N+ E HHE +DG GYP+G++ Sbjct: 74 LLTKPGRISVEEYDVLKTHVQFGYEILNKISFP---FNLAEIVHCHHEYLDGSGYPRGIS 130 Query: 455 REQMSPQARMMAIADIFEALTAADRPYKKGNTLSEALEIL---GGFRLRNHIDPDLFDVF 511 +++ +AR++ +ADI E+++ ADRPY+K +S AL+ + G RL + DV Sbjct: 131 GDEIPLEARILTVADIVESMS-ADRPYRKSLGMSAALDEIQRQSGVRLDAVVVDACLDVV 189 Query: 512 VRS 514 RS Sbjct: 190 KRS 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,574,635 Number of extensions: 62368 Number of successful extensions: 207 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 9 Length of query: 543 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 453 Effective length of database: 1,193,720 Effective search space: 540755160 Effective search space used: 540755160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)