BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0245.0016.p
         (471 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ...   578   e-166

>gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 569

 Score =  578 bits (1491), Expect = e-166,   Method: Compositional matrix adjust.
 Identities = 288/480 (60%), Positives = 366/480 (76%), Gaps = 11/480 (2%)

Query: 2   ALEALEAAYRKAGYSAVQVNVPEQELTSGVVTINISESVIGQITVSDNKYFSEQNIRNSL 61
           ALEALE  YR AGY+AVQV+VPEQEL+SG V I++ E+V+G++ VS+N++FS+ NIR S+
Sbjct: 91  ALEALENLYRSAGYTAVQVHVPEQELSSGEVRIDVVETVVGKVQVSENRHFSQANIRASI 150

Query: 62  PRLQVGKSPNLTAISQAVQLSNDNPAKQVNVALSVSDEEGKVDADVKVTDYNPLRVFMTV 121
           P L+ G  PNL  IS++VQL+NDNPAKQVNV LS +    ++DA ++V D +PLRV + +
Sbjct: 151 PALKEGVPPNLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAKIEVKDDDPLRVMLNL 210

Query: 122 DNTGAPATGNWRTGIAIQHANLFDRDQVGTLAYTTSPDSPSGVKVNLYSLGYRIPLYTLG 181
           DNTG+  TG WRTG+AIQH+NLF+RD V T+AYTTSPDSPSG KV+LYSLGYR+PLY LG
Sbjct: 211 DNTGSADTGRWRTGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKVDLYSLGYRLPLYELG 270

Query: 182 DSIEFIYGKSSVNTPGSVGAPNGLIGFTGKGDVVGLRWNHFFAREGESTSKLVAGLDYKK 241
           DS++FIYGKSSV++ G   A N  +  TG+G+V  LRWNHFFAR GE +SKLV G DYKK
Sbjct: 271 DSLDFIYGKSSVSS-GQTLALNSTLNITGRGEVYALRWNHFFARNGEWSSKLVLGADYKK 329

Query: 242 IDSRCDFAGASLN---IGSCVAYKTMPLSLTYSGQTRSATQNVDYNIGISRNIAIGPSYT 298
           +DS C   G  LN   + +C+ Y T+PLSLTYS Q +   + +D NIG++RN+A G +  
Sbjct: 330 VDSSCAINGGLLNGATLNTCLPYSTLPLSLTYSAQRQGVGEVIDANIGVARNMATGQARR 389

Query: 299 GSNGR-SDRYSYVT-GRDSADNFVIVRGAASWFKAFANDWQMRLAGTAQITNNALVSAEQ 356
               + +DRYS  T GR S DNF+ VRG ASW KAF NDWQMRLAG  Q++ +AL + EQ
Sbjct: 390 NDALQVTDRYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSPHALPAVEQ 449

Query: 357 FGLAGSTAVRGFTERAVAADGGVIVNAEVYTPELLP----KGSLRLLAFYDIGRGYNNNV 412
           FGLAG+TAVRGFTERAV+AD GV++N+E+YTP+LL     KGS+RLLAF D GRG NNNV
Sbjct: 450 FGLAGATAVRGFTERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDAGRGANNNV 509

Query: 413 GGGSVVNS-VTVSSMGIGARYSFSRDFNVKLDVARVSVRGTSSTEKRGDLNAHISAILGF 471
              S V S +T++S+G G RY+  RDFN++LDVARV + G S TE+RGDLNAH+SA LGF
Sbjct: 510 TANSQVQSTLTLASIGAGLRYALGRDFNLRLDVARVLLNGNSITEQRGDLNAHLSATLGF 569


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.132    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,398,755
Number of extensions: 60176
Number of successful extensions: 139
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 135
Number of HSP's successfully gapped: 1
Length of query: 471
Length of database: 1,619,870
Length adjustment: 89
Effective length of query: 382
Effective length of database: 1,198,455
Effective search space: 457809810
Effective search space used: 457809810
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 132 (55.5 bits)