BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0245.0008.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310319|ref|YP_003774411.1| TonB-dependent siderophore rece... 171 2e-43 gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore rece... 129 5e-31 gi|300310762|ref|YP_003774854.1| outer membrane ferric sideropho... 107 3e-24 gi|300311911|ref|YP_003776003.1| TonB-dependent siderophore rece... 105 5e-24 gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisideropho... 102 8e-23 >gi|300310319|ref|YP_003774411.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 723 Score = 171 bits (432), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 147/446 (32%), Positives = 213/446 (47%), Gaps = 39/446 (8%) Query: 26 ELPFSVQSYSSELIDAQESRTMMDVLKNDPSV-QDATQAGAYEMVRIRGYFSDWTNTVRR 84 + P SV +S + Q+SR + DV+KND S+ ++ G YE IRG+ D + R Sbjct: 71 DTPASVSVVTSAQMKDQQSRLLSDVVKNDASINENYAPVGYYENFSIRGFTLDPASAYRV 130 Query: 85 DGMSVAPYYEIPLENVEQIDLLKGPSGFLYGINSPGGTINYAIKRPTRDRFTSIKTSLRS 144 +G++VA I LEN E+++ LKG +G G+ + GG +NY KRP R ++ T Sbjct: 131 NGLTVAGEQTIALENKERVEFLKGVAGLQAGVVAAGGLVNYVTKRPANVRSITLGT---- 186 Query: 145 NSSGGSYVALDTGGPLDDGR-FGYRFNVARERNGDFRHNGDTSRDFVSGAFDWAINPDLL 203 +S+G Y+A D G + + FG R N A E + NGD RDF S + WAI+ L Sbjct: 187 DSNGSRYLATDLGALFGEHKQFGLRINAAHEDIHSYVSNGDGWRDFASLSATWAISARTL 246 Query: 204 VRANFDYQERKISAQPSIGPY--LNGQLPPVGSIDPRTLLG-QPWLQYETRTFNIGADLQ 260 V+ + +YQ++ AQ S+ Y L G P G + P T+L QPW Q R ++ A L+ Sbjct: 247 VQFDAEYQQK---AQRSVAGYQLLGGATLPSG-VSPSTMLTPQPWSQ-PVRDSSVNARLR 301 Query: 261 Y--RINDKWKLTTRAAQSYNGRVAAFPDVY-------------NVAANGNVLSGDIYYNP 305 N W+ +A GR A D Y AANG+ D Y P Sbjct: 302 IDTEFNADWRGYVQA-----GRSRAVIDDYLAFPYGSGNGASPTFAANGDFDVYD-YRVP 355 Query: 306 NQSFRVISTDSYVSGDFETIGIRHQLVTGFS-TRNFQAVESGFRVLPDTVGNIYNPSFTS 364 N R + G F + ++H+L G + +R G VL GNIYN + Sbjct: 356 NDERRNDEVQAVAIGKFASGWVQHELTLGMAMSRRIVDKSDGISVLVGQ-GNIYNGTPVL 414 Query: 365 APSNLTYPNHNKS-KNYQPSVFISDLMSLSDRWDVMLGLRHVRYKNDSMPASGKNTSQTA 423 +N P ++ + Q ++F SD M S RW V+ G R V S ++G T T+ Sbjct: 415 PAANAVIPAAYRNLDSRQKALFFSDRMQFSQRWQVLAGGRQVWLNEQSFNSTGAVTRDTS 474 Query: 424 -SINAPSAAVTFKVTPALSTYLSYAE 448 ++ P A+ FK LS Y SYAE Sbjct: 475 RNLFLPQLALIFKPVQNLSLYGSYAE 500 >gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 129 bits (324), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 118/442 (26%), Positives = 193/442 (43%), Gaps = 29/442 (6%) Query: 25 EELPFSVQSYSSELIDAQESRTMMDVLKNDPSVQDATQ-AGAYEMVRIRGYFSDWTN--- 80 +ELP SVQ S +LID + + DVL V G ++ IRG FS N Sbjct: 66 QELPQSVQVVSRQLIDDLAATRLDDVLLYVSGVARQNNFGGLWDNYSIRG-FSGSENGGM 124 Query: 81 TVRRDGMSVAPYYEIP--LENVEQIDLLKGPSGFLYGINSPGGTINYAIKRPTRDRFTSI 138 + +G + Y P + NVE +D LKGP+ LYG + PGGTIN K+P ++ Sbjct: 125 NILWNGFASNRGYAPPRDMANVESVDFLKGPAAALYGNSEPGGTINVVTKKPQFKSANAL 184 Query: 139 KTSLRSNSSGGSYVALDTGGPLDDGRFGYRFNVARERNGDFRHNGDTSRDFVSGAFDWAI 198 S+ SN + S A+D+ G L YR N A E G R + R+F + AF W + Sbjct: 185 DLSIGSNDAYRS--AVDSTGALSS-TVAYRLNAAMEHKGSMRDQVRSEREFFAPAFTWLL 241 Query: 199 NPDLLVRANFDYQERKISAQPSIGPYLNGQLPPVGSIDPRTLLGQP-----WLQYETRTF 253 + ++ ++ +K I +NG G + LG+P +Q + Sbjct: 242 TENTILNYEGEFLRQKAPLDRGI-VAVNGN---AGVLPVSRFLGEPNDGDIKMQNQNHLL 297 Query: 254 NIGADLQYRINDKWKLTTRAAQSYNGRVAAFPDVYNVAANGNVLSGDIYYNPNQSFRVIS 313 L+ R+N+ W L T + + + A+ L Y + S+ S Sbjct: 298 T----LEQRLNENWHLRTGIFHKEGSLDGYSTEAFALQADNATLRRQRRYR-DYSWHDTS 352 Query: 314 TDSYVSGDFETIGIRHQLVTGFSTRNFQAVESGFRVLPDTVG----NIYNPSFTSAPSNL 369 + + G F T G+ H+++ G + R+ P V NIYNP + A Sbjct: 353 LQAEIGGKFATAGVGHEVLVGTEVDWLTLDQRMLRINPSNVAPYAVNIYNPVYGQAQPTP 412 Query: 370 TYPNHNKSKNYQPSVFISDLMSLSDRWDVMLGLRHVRYKND-SMPASGKNTSQTASINAP 428 T + +++ D +SLS+RW ++ G+R +++ + +G T Q +P Sbjct: 413 TANTDTSERQISTGLYLQDQLSLSERWRLLAGVRADAFRSTLNNLRNGAVTRQDKHAYSP 472 Query: 429 SAAVTFKVTPALSTYLSYAEGF 450 +T+ +TP+ S Y+S ++ F Sbjct: 473 RLGLTYLLTPSWSVYVSASKSF 494 >gi|300310762|ref|YP_003774854.1| outer membrane ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 717 Score = 107 bits (266), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 116/447 (25%), Positives = 194/447 (43%), Gaps = 32/447 (7%) Query: 24 PEELPFSVQSYSSELIDAQESRTMMDVLKNDPSVQDATQAGAYEMVRIRGYFSDWTNTVR 83 P E+P S+QS S E + +RT+ L P + + ++ RIRG+ S N + Sbjct: 77 PMEVPQSIQSVSVEQVSVYGARTLAGALLGVPGISSISDT-RFDAFRIRGFSS--ANDLL 133 Query: 84 RDGMSVAPYYEIPLENVEQIDLLKGPSGFLYGINSPGGTINYAIKRPTRDRFTSIKTSLR 143 DGM P Y L N+E++++L+GP+ LYG S GG IN K+P +D + + +L Sbjct: 134 LDGMRDDPQYVRGLGNIERVEVLRGPASVLYGRGSGGGVINRISKQPGQD--VASQATLS 191 Query: 144 SNSSGGSYVALDTGGPLDDGRFGYRFNVARERNGDFRHNGDTSRDFVSGAFDWAINPD-L 202 S+G ALD PL + R N RE G FR + +R + + A W D Sbjct: 192 LGSAGLLGAALDWNRPLAP-HWALRINTGREHAGSFRDTVNGTRQYFAPALKWESGRDSW 250 Query: 203 LVRANFDYQERKISAQPSIGPYLNGQLPPVGSIDPRTLLGQPWLQYETRTFNIGADLQYR 262 L++A +D ER P G + G+ + +P T P +E G D+ Sbjct: 251 LLQAEYDEYERV----PDRG--IPGRAIATANGEP-TAYALPSAGHEQFFGAAGRDVIRD 303 Query: 263 INDKWKLTTRAAQSYNGRVAAFPDVYNVAAN-GNVLSGDIYYNPNQSFRVISTDSY---- 317 I+ + T A S + + + ++ +N N Y + + Y Sbjct: 304 IHMSLRSTLTRALSQDWTLRHALALMDLNSNFDNTFVTQAYQSAPLDLDRVQRTRYQQNL 363 Query: 318 ----VSGDFETIGI--RHQLVTGFSTRNFQAVESGFRVLPDTVGNIYNPSFTSAPSNLTY 371 V + E +G H L+ G ++Q E + + P T + T Sbjct: 364 QQRSVQNNLELLGQLGAHDLLLGVE-HSWQKREPRLWSVAAPAVSAVTPENTRNGDDPTT 422 Query: 372 P---NHNKSKNYQPSVFISDLMSLSDRWDVMLGLRHVRYKNDSM-PASGKNTSQTASINA 427 P N++K+ N +++ D ++L+ W ++ GLR R++ DS +G + +TA + Sbjct: 423 PFQMNYHKASNL--GLYVQDKITLAAHWKMLAGLRWDRFEVDSRNRRNGVRSERTAHALS 480 Query: 428 PSAAVTFKVTPALSTYLSYAEGFEQPG 454 P V ++ P YLS+++ F G Sbjct: 481 PRIGVVWEALPGQHAYLSWSKNFAPVG 507 >gi|300311911|ref|YP_003776003.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 733 Score = 105 bits (263), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 133/479 (27%), Positives = 196/479 (40%), Gaps = 67/479 (13%) Query: 13 GVNVGALGYKKPEELPFSVQSYSSELIDAQESRTMMDVLKNDPSVQDATQAGAY-EMVRI 71 G +G LG + P +Y+ +LI Q++ + DVLKNDP V+ G + E I Sbjct: 68 GSRLGVLGNVDNLDSPVRSVAYTQQLIADQQAHGVGDVLKNDPVVRTTRGFGNFQESYMI 127 Query: 72 RGYFSDWTNTVRRDGMSVAPYYEIPLENVEQIDLLKGPSGFLYGIN----SPGGTINYAI 127 RG+ ++ + + + P + E +E++++ G S FL G GGTIN Sbjct: 128 RGFIANSDDLMYNGLFGILPRQYVASEMMERVEVQYGASAFLNGATPGDAGLGGTINIVP 187 Query: 128 KRPTRDRFTSIKTSLRSNSSGGSYVALDTG---GPLDDGRFGYRFNVARERNGDFRHNGD 184 KR + T + S +S Y ALD GP + R G R N AR G N Sbjct: 188 KRAANEPLTDLTLGYESGNS--QYAALDVSRRFGPQE--RLGVRINAARHAGGTGVANER 243 Query: 185 TSRDFVSGAFDWAINPDLLVRANFDYQERKIS-AQPS--IGPYLNGQLP--PVGSIDPRT 239 + S D+ N D + A+ QE +S +PS IG L LP P GS + Sbjct: 244 RETNLFSLGVDYRGN-DFRLSADLGLQENNLSNVRPSVTIGAGLT-SLPVAPSGSAN--- 298 Query: 240 LLGQPWLQYETRTFNIGADLQYRINDKWKLT--TRAAQSYNGRVAAFPDVYNVAANGNVL 297 GQPW R DL + +W L+ T A ++ R N L Sbjct: 299 -YGQPWSYSNAR------DLFGTVRGEWDLSSSTTAWAAFGARQGT---------ENNSL 342 Query: 298 SGDIYYNPNQS------FRVISTDSYVSGDFETIGIRHQLVTGFSTRNFQAVESGFRVLP 351 SG N + F D +G+ +G+R L TG + A S + + Sbjct: 343 SGVRVTNASTGAAATYRFDNARQDDVATGE---VGVRTHLQTGAVSHKLVAAASAYSLNS 399 Query: 352 DTV----------GNIYNPS------FTSAPSNLTYPNHNKSKNYQPSVFISDLMSLSDR 395 N+YNP+ TS ++L P N +V L DR Sbjct: 400 RNAYGLSPSYALATNLYNPTNYAQPALTSLGNSLADPQTTIKTNLLSAVVSDTLGFYDDR 459 Query: 396 WDVMLGLRHVRYKNDSMPASGK-NTSQ-TASINAPSAAVTFKVTPALSTYLSYAEGFEQ 452 V +G+R + K+D+ + + NT+ S +P A V FK+T LS Y +Y EG +Q Sbjct: 460 VLVTVGVRQQQLKSDTYAYNTQANTAHYDQSATSPMAGVVFKLTKTLSVYGNYIEGLQQ 518 >gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisiderophore receptor signal peptide protein, tonB-dependent receptor [Herbaspirillum seropedicae SmR1] Length = 715 Score = 102 bits (253), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 120/462 (25%), Positives = 201/462 (43%), Gaps = 70/462 (15%) Query: 26 ELPFSVQSYSSELIDAQESRTMMDVLKNDPSVQDATQAG--AYEMVRIRGYFSDWTNTVR 83 E P S+ + ++D+Q++ ++ D LKN V A Q G ++ + IRG + ++++ Sbjct: 79 ETPQSISVVTRAMMDSQQAVSLSDALKNVSGVV-AQQYGRRGWDDLIIRGQVA--SDSLY 135 Query: 84 RDGMSVAPYYEIP--LENVEQIDLLKGPSGFLYGINSPGGTINYAIKRPTRDRFTSIKTS 141 DG+ + L ++Q+++LKGP LYG+ PGG +N KRP F ++ + Sbjct: 136 LDGLRTTASSRVAEQLYGLQQVEVLKGPGSLLYGLVLPGGLVNMVSKRPQGSDFANVDVT 195 Query: 142 LRSNSSGGSYVALDTGGPLDDGRFGYRFNVARERNGDFRHNGDTSRDFVSGAFDW----- 196 + S+ S V L+ ++G+ +R N N D + D V W Sbjct: 196 IGSHDLYQSTVDLNKSLS-ENGKQAFRIN-------GLISNSDDATDHV-----WFKNRY 242 Query: 197 ---AINPDLLVRANF----DYQERKISAQPSIGPYLNGQLPPVGSIDP-------RTLL- 241 +++ DL R +F YQER Q LP GSI P R+L Sbjct: 243 IAPSLSLDLGARTDFTILTSYQERNYVRQQG--------LPLSGSITPNASGPLARSLFI 294 Query: 242 ----GQPWLQYETRTFNIGADLQYRINDKWKLTTRAAQSYNGRVAAFPDVYNVAANGNVL 297 +P+ ETR IG L +R +D W L N R F + + N+ Sbjct: 295 GEPGARPYAANETR---IGYSLTHRFDDGWTLNN------NLRYQQFTMSGQLISINNLT 345 Query: 298 SGDIYYNP-NQSF--RVISTDSYVSGDFETIGIRHQLVTGFSTRNFQAVESGFRVLPDTV 354 + + +Q+F + + D+ V F T +H++ G N + +E + Sbjct: 346 GTSLTRSAMDQTFEGQTFTMDTNVQRSFATAWGKHEVTLGTDYMNSRELERTLTCSVTPL 405 Query: 355 GNIYNPSFTS---APSNLTYPNH--NKSKNYQPSVFISDLMSLSDRWDVMLGLRHVRYKN 409 NIYNPS+ + P+ LT N N + + D +SL +RW ++ G+R R Sbjct: 406 SNIYNPSYGARVVCPTQLTSTNAKLNDTSVRDTGFYARDKISLGERWQLLAGVRQDRAAT 465 Query: 410 DSMPA-SGKNTSQTASINAPSAAVTFKVTPALSTYLSYAEGF 450 + A +G ++ +S SAA+ ++ + YLSYA F Sbjct: 466 YTTSALTGSHSDNASSATTASAAIMVELWHGVRPYLSYATSF 507 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,554,403 Number of extensions: 67441 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 153 Number of HSP's successfully gapped: 16 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)