BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0245.0001.p (251 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 69 3e-13 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 66 2e-12 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 66 3e-12 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 59 4e-10 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 59 6e-10 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 69.3 bits (168), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 62/183 (33%), Positives = 95/183 (51%), Gaps = 18/183 (9%) Query: 54 RSERMHRIMLGSDSEKAQKIA--IVAAVV----AMLLFSLVVVA-TLHRSLKPLRKASAA 106 R R+ + +G EK ++A I+ V+ +L +L +V L R L PL + Sbjct: 48 REPRLATVQVGETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQR 107 Query: 107 AEKIRIDNLSARLSLEDVPSELAPLFNAFNLALQRLEDGYRVQREFLATAAHELKTPLAL 166 R D+LS + VP E++PL + N L RL +Q+ F+A AAH++KTPLA Sbjct: 108 IRARRPDDLSP-IDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAG 166 Query: 167 MRGQIELDER-GDRSALLRDLDHMA-------RHVHQLLHLAEASERQNYA--MERIDLK 216 MR Q EL R D+ + R L+ +A R V+QLL LA A +Q+ + M ++L Sbjct: 167 MRMQSELALRQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELN 226 Query: 217 DIA 219 ++A Sbjct: 227 ELA 229 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 66.2 bits (160), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 52/160 (32%), Positives = 81/160 (50%), Gaps = 16/160 (10%) Query: 61 IMLGSDSEKA----QKIAIVAAVVAMLLFSLVVVATLH---RSLKPLRKASAAAEKIRID 113 + +G D E + + +V ++L L V+++ L PLR+ S A+ Sbjct: 150 VTVGVDGEPYFEMRRTFLLATSVFSLLGIVLAVISSYWIARAGLAPLRQMSDRAQSFSPR 209 Query: 114 NLSARLSLEDVPSELAPLFNAFNLALQRLEDGYRVQREFLATAAHELKTPLALMRGQIEL 173 LSAR+ +E +P EL+ L AFN AL R+ED Y F A AHEL+TPLA + G+ ++ Sbjct: 210 KLSARMPVEAMPQELSVLALAFNGALDRIEDAYTQLEAFNADVAHELRTPLANLIGETQV 269 Query: 174 DERGDRSA---------LLRDLDHMARHVHQLLHLAEASE 204 +RSA L +LD + V+ +L LA + + Sbjct: 270 ALARERSAGEFKDVLQSNLEELDRIRSIVNDMLFLARSDQ 309 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 55/158 (34%), Positives = 81/158 (51%), Gaps = 17/158 (10%) Query: 84 LFSLVVVATLHRSLKPLRKASAAAEKIRIDNLSARLSLEDV--PSELAPLFNAFNLALQR 141 + S V V+ R L P+RKA+A ++ ++++ L DV P E+ PL +A N L R Sbjct: 155 VLSWVAVS---RGLAPVRKAAA---EVAARDMNSLQPLADVGMPQEIQPLTHALNDLLAR 208 Query: 142 LEDGYRVQREFLATAAHELKTPLALMRGQIELDERG----DRSALLRDLDH----MARHV 193 L+ QR F+A AAHEL+TPL +R Q +L ER +R A DL + V Sbjct: 209 LKHATETQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFADLKNGLERTTHLV 268 Query: 194 HQLLHLAEASERQNYAMERIDLKDIAAEASAQLERLAQ 231 HQLL LA E + ++DL+ + L +A+ Sbjct: 269 HQLLTLAR-QEPDAMSFTQVDLRALLGSVVGDLSSVAE 305 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 58.9 bits (141), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 48/147 (32%), Positives = 77/147 (52%), Gaps = 12/147 (8%) Query: 98 KPLRKASAAAEKIRIDNLSARLSLEDVPSELAPLFNAFNLALQRLEDGYRVQREFLATAA 157 +PL S A +I D+LSARL E +P+EL + +FN L RLE GY +F A Sbjct: 200 QPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRLEAGYAHLAQFSGDLA 259 Query: 158 HELKTPLALMRGQIELDERGDRS---------ALLRDLDHMARHVHQLLHLAEASERQNY 208 HE++TP+ ++ GQ ++ RS + + +L ++R V +L LA+A + Sbjct: 260 HEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIVENILFLAQA-DHAAL 318 Query: 209 AMER--IDLKDIAAEASAQLERLAQQR 233 A+ER +DL + + E +A +R Sbjct: 319 AVERNALDLAEELGRITDYFEGIADER 345 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 58.5 bits (140), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 42/128 (32%), Positives = 72/128 (56%), Gaps = 8/128 (6%) Query: 75 IVAAVVAMLLFSLVVVATLHRSLKPLRKASAAAEKIRIDNLSARLSLE--DVPSELAPLF 132 I+ AVVA++L + +VV +PL + + A ++ + + R+ +E D LA F Sbjct: 175 IIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDF 234 Query: 133 NAFNLALQRLEDGYRVQREFLATAAHELKTPLALMRGQIELDERG---DRSALLRDLDHM 189 N + L+R E +++REF+A +HEL+TPLA++ GQ+E E G + LR L Sbjct: 235 NQLAMTLERNE---KLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSE 291 Query: 190 ARHVHQLL 197 H+++L+ Sbjct: 292 VEHLNKLV 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.132 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 657,582 Number of extensions: 23024 Number of successful extensions: 121 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 124 Number of HSP's successfully gapped: 13 Length of query: 251 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 168 Effective length of database: 1,226,865 Effective search space: 206113320 Effective search space used: 206113320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)