BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0006.p (138 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313111|ref|YP_003777203.1| TRAP-type C4-dicarboxylate tran... 104 3e-24 gi|300310440|ref|YP_003774532.1| TrapT dctQ-M fusion permease, d... 79 2e-16 >gi|300313111|ref|YP_003777203.1| TRAP-type C4-dicarboxylate transport system dctQ-M fusion permease large permease [Herbaspirillum seropedicae SmR1] Length = 627 Score = 104 bits (259), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 53/53 (100%), Positives = 53/53 (100%) Query: 33 EMKKRGSKPEELVALLSSSGAMTETIPPSLVLITIGAVCSVSITALFIGGLMP 85 EMKKRGSKPEELVALLSSSGAMTETIPPSLVLITIGAVCSVSITALFIGGLMP Sbjct: 328 EMKKRGSKPEELVALLSSSGAMTETIPPSLVLITIGAVCSVSITALFIGGLMP 380 Score = 82.4 bits (202), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 54/64 (84%), Positives = 56/64 (87%), Gaps = 2/64 (3%) Query: 73 VSITALFIGGLMPAPPFGVGFYAACAIGKVSPDKVFNRVWGYLAALVVALLVVAAVPWIS 132 V I A+ IG + APPFGVGFYAACAIGKVSPD VFNRVWGYLAALVVALLVVA VPWIS Sbjct: 566 VVILAMGIG--LFAPPFGVGFYAACAIGKVSPDGVFNRVWGYLAALVVALLVVAFVPWIS 623 Query: 133 IGFL 136 IGFL Sbjct: 624 IGFL 627 >gi|300310440|ref|YP_003774532.1| TrapT dctQ-M fusion permease, dicarboxylate transport protein [Herbaspirillum seropedicae SmR1] Length = 618 Score = 78.6 bits (192), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 35/51 (68%), Positives = 40/51 (78%) Query: 86 APPFGVGFYAACAIGKVSPDKVFNRVWGYLAALVVALLVVAAVPWISIGFL 136 APPFG+G+YAAC IG+V PD R+W YL ALVV LL+VA VPW SIGFL Sbjct: 568 APPFGLGYYAACTIGRVHPDVAVKRIWPYLGALVVGLLIVAFVPWFSIGFL 618 Score = 77.4 bits (189), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 37/53 (69%), Positives = 45/53 (84%) Query: 33 EMKKRGSKPEELVALLSSSGAMTETIPPSLVLITIGAVCSVSITALFIGGLMP 85 EMK+RG+ EL++LL++SGAM ETIPPSLVLITIG+V VSI ALF GG+MP Sbjct: 323 EMKRRGNHEGELISLLAASGAMAETIPPSLVLITIGSVAGVSIAALFTGGIMP 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 384,534 Number of extensions: 12849 Number of successful extensions: 64 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 64 Number of HSP's successfully gapped: 4 Length of query: 138 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 62 Effective length of database: 1,260,010 Effective search space: 78120620 Effective search space used: 78120620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)