BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0003.p (265 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 451 e-128 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 145 2e-36 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 142 3e-35 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 135 3e-33 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 134 5e-33 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 451 bits (1161), Expect = e-128, Method: Compositional matrix adjust. Identities = 205/264 (77%), Positives = 245/264 (92%) Query: 1 MARAMEDKVVIVSGAGSVGEGWGNGKAAAVLYAREGARVLAVDRNLQAAQETCDLIRSEG 60 M + M+ K +IV+GAGSVGEGWGNGKAAAVLYAREG RVLAVDR+++AA +T D+IRSEG Sbjct: 1 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEG 60 Query: 61 GVCEVVAADVSRTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGPVEASEESWNRVIAIN 120 GVCEV+AADVSR +++A+ +AA+DHFGR+DVLHNNVGIA+ GGPVE SEESWNR++A+N Sbjct: 61 GVCEVLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN 120 Query: 121 QTSVFMTCKHVLPVMEKQKKGAIVNISSLAAIRWVGFPYVAYSASKAAMLALTKNIAMQY 180 QTS+F+T KHVLP+MEKQ+KGAIVNISSLAA+RW+GFPY+ Y+A+KAA+LA TKN+AMQY Sbjct: 121 QTSLFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 Query: 181 APSGIRANCVLPGLMDTPMIREPLKASYGGDIEEMRRKRHAQCPMGHMGDAWDVAHAALF 240 AP GIRANCVLPG+MDTPMIREPLKASYGGDIE+MR KR+AQCPMG MGDAWDVAHA+LF Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 241 LASDAARYVTGVDLIVDGGLSLKC 264 LASD ARY+TG+D+IVDGGLSL+C Sbjct: 241 LASDHARYITGLDMIVDGGLSLRC 264 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 145 bits (367), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 94/263 (35%), Positives = 144/263 (54%), Gaps = 15/263 (5%) Query: 5 MEDKVVIVSGAGSVGEGWGNGKAAAVLYAREGARVLAVDRNLQAAQETCDLIRSEGGVCE 64 ++DKV IV+G GS + A YAREGA ++ D + I++ GG Sbjct: 3 LQDKVAIVTGGGSGFG-----EGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAV 57 Query: 65 VVAADVSRTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGP-VEASEESWNRVIAINQTS 123 ADVS+ D++ ++ AL+HFG++D++ NN G P +E E+ ++RV A+N S Sbjct: 58 FAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKS 117 Query: 124 VFMTCKHVLPVMEKQKKGAIVNISSLAAIR-WVGFPYVAYSASKAAMLALTKNIAMQYAP 182 +F++ K +P + GA +NI+S A IR G + Y+ SK A++ +K++A + P Sbjct: 118 IFLSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTW--YNGSKGAVITTSKSMAAELGP 175 Query: 183 SGIRANCVLPGLMDTPMIREPLKASYGGDIEEMRRKRHAQCPMGHMGDAWDVAHAALFLA 242 IR NCV P + T ++ E + D E R+K A PMG D+A+A L+L Sbjct: 176 DKIRVNCVNPVISATGLLSEFMGVP---DTPENRKKFVATIPMGRFSTPEDIANACLYLG 232 Query: 243 SDAARYVTGVDLIVDGGLSLKCV 265 SD A +VTGV + VDGG +CV Sbjct: 233 SDEAEFVTGVCIEVDGG---RCV 252 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 142 bits (357), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 95/259 (36%), Positives = 133/259 (51%), Gaps = 18/259 (6%) Query: 5 MEDKVVIVSGAGSVGEGWGNGKAAAVLYAREGARVLAVDRNLQAAQETCDLIRSEGGVCE 64 ++ KV +++GA + G G+A A+L+AR GA ++ R A ++ LI ++GG Sbjct: 4 LQGKVALITGASA-----GIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRAS 58 Query: 65 VVAADVSRTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGPV-EASEESWNRVIAINQTS 123 VVA DV E V ALD FG +D+ NN G P+ E S + W + N TS Sbjct: 59 VVAGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTS 118 Query: 124 VFMTCKHVLPVMEKQKKGAIVNISSLAAIRWVGFP-YVAYSASKAAMLALTKNIAMQYAP 182 F+ + +P M + GAIV SS G P AY A+KA ++ L K IA YA Sbjct: 119 AFLGARCQIPAMLARGAGAIVFTSSFVG-SSAGLPGMAAYGAAKAGLMGLVKGIAADYAV 177 Query: 183 SGIRANCVLPGLMDTPMIREPLKASYGGDIEEMRRKRHAQCPMGHMGDAWDVAHAALFLA 242 GIRAN +LPG +DT M +P + + + M+R + ++A AALFLA Sbjct: 178 QGIRANALLPGGVDTDMGGDPQQKQWAAGLHAMKRIAQPE----------EIAAAALFLA 227 Query: 243 SDAARYVTGVDLIVDGGLS 261 S A +VTG L DGG S Sbjct: 228 SPMASFVTGTALYADGGNS 246 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 135 bits (341), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 86/259 (33%), Positives = 137/259 (52%), Gaps = 18/259 (6%) Query: 5 MEDKVVIVSGAGSVGEGWGNGKAAAVLYAREGARVLAVDRNLQAAQETCDLIRSEGGVCE 64 ++DKV +++G + G G A A L A +GARV+ +D I S+ Sbjct: 3 LKDKVAVITGGAGIN---GLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQ---HL 56 Query: 65 VVAADVSRTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGPVEASEESWNRVIAINQTSV 124 + ADV+ E A L FGRID+L NN GI + +E + ++R++ ++ Sbjct: 57 GLVADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGT 116 Query: 125 FMTCKHVLPVMEKQKKGAIVNISSLAAIRW---VGFPYVAYSASKAAMLALTKNIAMQYA 181 + VLP M Q+ G+I+ ISS++A R +G P+ YSA+KA +L L + +A ++ Sbjct: 117 LYMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREFG 174 Query: 182 PSGIRANCVLPGLMDTPMIREPLKASYGGDIEEMRRKRHAQCPMGHMGDAWDVAHAALFL 241 P GIR N + PGL+ T +I+ L EE + + P+ +G A D+ A +FL Sbjct: 175 PEGIRVNSITPGLIGTDIIKGKLT-------EEKKAEIAETIPLARLGRADDIGGACVFL 227 Query: 242 ASDAARYVTGVDLIVDGGL 260 SD ++Y TG+ L V+GG+ Sbjct: 228 GSDLSQYCTGITLDVNGGM 246 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 134 bits (338), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 92/260 (35%), Positives = 146/260 (56%), Gaps = 20/260 (7%) Query: 5 MEDKVVIVSG-AGSVGEGWGNGKAAAVLYAREGARVLAVDRNLQAAQETCDLIRSEGGVC 63 ++DKV I++G AG G G+ + A L A +GAR++ +D L+ A+ R G Sbjct: 3 LKDKVAIITGGAGQNGLGF----STARLMAAQGARIVILD--LERAEPAGAAARL-GAQH 55 Query: 64 EVVAADVSRTPDIEAMVQAALDHFGRIDVLHNNVGIAETGGPVEASEESWNRVIAINQTS 123 + ADV+ EA A L FGRID+L NN G+ + +E + + ++R++ ++ Sbjct: 56 LGLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRG 115 Query: 124 VFMTCKHVLPVMEKQKKGAIVNISSLAAIRW---VGFPYVAYSASKAAMLALTKNIAMQY 180 + VLP M+ Q+ G+IV ISS++A R +G P+ YSA+KA +L L + +A ++ Sbjct: 116 TLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREF 173 Query: 181 APSGIRANCVLPGLMDTPMIREPLKASYGGDIEEMRRKRHAQCPMGHMGDAWDVAHAALF 240 P GIR NC+ PGL+ T + ++ L A +I + P+G +G DV +F Sbjct: 174 GPQGIRVNCITPGLISTDINKDILSAEKKAEIAQT-------IPLGRLGGPDDVGGVCVF 226 Query: 241 LASDAARYVTGVDLIVDGGL 260 LASD ++Y TGV L V+GG+ Sbjct: 227 LASDLSQYCTGVTLDVNGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 799,701 Number of extensions: 31407 Number of successful extensions: 242 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 49 Length of query: 265 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 181 Effective length of database: 1,222,130 Effective search space: 221205530 Effective search space used: 221205530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)