BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0241.0024.p (301 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 122 4e-29 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 116 3e-27 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 114 9e-27 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 99 3e-22 gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 97 2e-21 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 122 bits (306), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 103/322 (31%), Positives = 149/322 (46%), Gaps = 41/322 (12%) Query: 1 VLVRIRAAGLNPLDAKIRAG-----KAGHAQAQLPAVLGIDMAGVVEAIGDGVSGFNVGD 55 VLVR+ A GLNP D +R G P +LG D++GVVEA+ D V F VGD Sbjct: 35 VLVRVHAVGLNPPDWYLREGYKMLPPEWQPTVAFPLILGTDISGVVEAVADDVKDFAVGD 94 Query: 56 EVYGM---TGGVGSVQGSLAQFAAVDADLLAHKPANIGMREAAALPLIFITAWEGLVDRA 112 EVY M G+ + AQ+ +V A +A KP I +AAA P+ +TAW+ +++ Sbjct: 95 EVYSMVRFPSGLAGGSRAYAQYVSVAASEIALKPQRISHAQAAAAPMSLLTAWQFMIELG 154 Query: 113 H--------------VRSGQKVLVHGGAGGVGHIAVQIARAFGADVYATGSAEQRDLITG 158 H + V+V+G AGGVGH A+Q+A+ GA V A GS + Sbjct: 155 HDIQNPLQPHRHEPVPLKDKTVVVNGAAGGVGHFALQLAKWKGARVIAIGSGGHEAFLRD 214 Query: 159 YGA-TFIDYRASTVEEYVQQYTGGEGFDIVYDTVGGATLDASFIAAKRYHGHVVSCLGWG 217 GA FIDY S E+ + D+V D VGG KR G Sbjct: 215 LGADEFIDYTRSAAEDVLSD------VDLVIDAVGGPASGRFLRTLKR---------GGA 259 Query: 218 THALAPLSFRAATYSGVFTLMPLLTGKGRAHHGEIMEQATRLIEAGKIVVQVDQRHFGFD 277 + PL F A + + + T + R+ G ++Q L+++G + V +D F Sbjct: 260 LFPIFPLGFSGAEEAARLGVN-VSTTQVRS-SGAQLQQLAGLLDSGVVRVAIDST-FALQ 316 Query: 278 QADAAYDVIDAGGARGKLVIEI 299 A A++ G RGK+V+ + Sbjct: 317 DAGKAHERAAQGHIRGKIVLSV 338 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 116 bits (290), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 104/307 (33%), Positives = 150/307 (48%), Gaps = 19/307 (6%) Query: 1 VLVRIRAAGLNPLDAKIRAGK--AGHAQAQLPAVLGIDMAGVVEAIGDGVSGFNVGDEVY 58 VL+++ AAG+N D R G + LP G+++AG + A G SGF +GD V Sbjct: 30 VLIKVHAAGVNRPDVFQRTGNYPVPPGASDLP---GLEVAGEIVAGDLGDSGFKLGDLVC 86 Query: 59 GMTGGVGSVQGSLAQFAAVDADLLAHKPANIGMREAAALPLIFITAWEGLVDRAHVRSGQ 118 + G G A+F A P + EAAALP + T W + DR + + Sbjct: 87 ALVQG-----GGYAEFCAAPLAQCLPVPKGLSALEAAALPENYFTVWSNVFDRGQLSGEE 141 Query: 119 KVLVHGGAGGVGHIAVQIARAFGADVYAT-GSAEQRDLITGYGATF-IDYRASTVEEYVQ 176 +LV GG+ G+G A+QIARA G V+AT GSAE+ GA I+Y+ E V+ Sbjct: 142 TLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKCRACEELGAERAINYKTEDFEAVVK 201 Query: 177 QYTGGEGFDIVYDTVGGATLDASFIAAKRYHGHVV--SCLG--WGTHALAPLSFRAATYS 232 + TGG G D++ D VGG L I A G +V + LG G + + R T + Sbjct: 202 ELTGGRGVDVILDMVGGDYLPRE-IKALADDGRLVFIAQLGGAKGQLDMGQVMRRRLTIT 260 Query: 233 GVFTLMPLLTGKGRAHHGEIMEQATRLIEAGKIVVQVDQRHFGFDQADAAYDVIDAGGAR 292 G TL P A ++ E LIEAGKI + R F +QA A+ +++ Sbjct: 261 GS-TLRPRPVAFKAAIAAKLREHVWPLIEAGKIRPVIHHR-FPLEQAAQAHAMMEGSTHI 318 Query: 293 GKLVIEI 299 GK+++E+ Sbjct: 319 GKIMLEL 325 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 114 bits (285), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 100/290 (34%), Positives = 140/290 (48%), Gaps = 16/290 (5%) Query: 3 VRIR--AAGLNPLDAKIRAGKAGHAQAQLPAVLGIDMAGVVEAIGDGVSGFNVGDEVYGM 60 VRIR A GLN +D RAG LPA LG + AG +EA+G GVSGF VGD V Sbjct: 31 VRIRHAAVGLNFIDCYFRAGLYPQP---LPAGLGQEAAGTIEAVGAGVSGFKVGDRV--- 84 Query: 61 TGGVGSVQGSLAQFAAVDADLLAHKPANIGMREAAALPLIFITAWEGLVDRAHVRSGQKV 120 G G+ A+ + AD+L P I AAA+ L +T L V+ G + Sbjct: 85 -AYAGRPNGAYAEERVMPADILVKLPDAISFDTAAAMMLQGMTVQYLLRQTYAVQPGDTI 143 Query: 121 LVHGGAGGVGHIAVQIARAFGADVYATGSAEQR-DLITGYGAT-FIDYRASTVEEYVQQY 178 L H AGG+G IA Q ARA G ++ T S++++ L +GA I+Y+ E V++ Sbjct: 144 LFHAAAGGIGLIACQWARALGVNLIGTVSSDEKAALALQHGAAHVINYKTENFVERVKEL 203 Query: 179 TGGEGFDIVYDTVGGATLDASFIAAKRYHGHVVSCLGWGTHALAPLSFRAATYSGVFTLM 238 TGG G +VYD++G T S + R G +VS G + +AP G L Sbjct: 204 TGGRGVQVVYDSIGKDTFLGS-LDCLRPRGMMVS-FGNASGPVAPFGVSELASRGSLFLT 261 Query: 239 PLLTGKGRAHHGEIMEQATRLIE---AGKIVVQVDQRHFGFDQADAAYDV 285 G + E+ A L + KI ++++QR+ D A A D+ Sbjct: 262 RPSLGNYTSTRAELDATAGDLFDMVLGNKISIEINQRYALKDVAQAHADL 311 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 99.4 bits (246), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 73/207 (35%), Positives = 108/207 (52%), Gaps = 11/207 (5%) Query: 1 VLVRIRAAGLNPLDAKIRAGKAGHAQA-QLPAVLGIDMAGVVEAIGDGVSGFNVGDEVYG 59 V V ++AAG+ P+D K+RAG+ H A LP V G D AGV+ A+G+GV VG+ V Sbjct: 30 VRVAMQAAGVAPVDTKLRAGRLQHLVALDLPKVPGRDGAGVITALGEGVHTLQVGEAVCV 89 Query: 60 MTGGVGSVQGSLAQFAAVDADLLAHKPANIGMREAAALPLIFITAWEGLVDRAHVRSGQK 119 M G +G+ + + + KP + M++AAAL +AW L+ A + +G K Sbjct: 90 MPSMAG--RGTTLAELVLPQEQVVRKPDVLSMQQAAALLQPACSAWIALMQTAPIAAGMK 147 Query: 120 VLVHGGAGGVGHIAVQIARAFGADVYATGSAEQRDLITGYGATFIDYRASTVEEYVQQYT 179 V +H G+G VG + VQ+A GA+V A+ + + + G AS V Y + Sbjct: 148 VFIHAGSGAVGSLMVQLAAHLGAEVTASCRSSNVEYVAALG-------ASRVLAYDRDEL 200 Query: 180 GG-EGFDIVYDTVGGATLDASFIAAKR 205 G D+V D VGGA D S+ +R Sbjct: 201 GRVRDQDVVIDLVGGAAHDQSYRMLRR 227 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 89/308 (28%), Positives = 150/308 (48%), Gaps = 20/308 (6%) Query: 1 VLVRIRAAGLNPLDAKIRAGKAGHAQAQLPAVLGIDMAGVVEAIGDGVSGFNVGDEVYGM 60 +LV ++A +NP+D KIRA A+ VLG D++GVV A+G V+ F GDEV+ Sbjct: 34 LLVEVKAISVNPVDTKIRAS------AKEARVLGWDVSGVVRAVGPAVTLFAPGDEVF-- 85 Query: 61 TGGVGSVQGSLAQFAAVDADLLAHKPANIGMREAAALPLIFITAWEGLVDRAHVRS---- 116 G + G+ ++ VD ++ KP+++G +AAA+PL ITAWE L DR V Sbjct: 86 YAGSITRPGANSELHVVDERIVGRKPSSLGHADAAAIPLTAITAWELLFDRVAVAEGGGQ 145 Query: 117 GQKVLVHGGAGGVGHIAVQIARAFGA-DVYATGS-AEQRDLITGYGATFIDYRASTVEEY 174 GQ +L+ G AGGVG + Q+AR V T S E + GA + + ++ Sbjct: 146 GQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASRPETAHWVGELGAHHVIDHSQPLKPQ 205 Query: 175 VQQYTGGEGFDIVYDTVGGATLDASFIAAKRYHGHVVSCLGWGTHALAPLSFRAAT--YS 232 +++ G IV A I G + + PL ++ + + Sbjct: 206 LEKL-GIPEVGIVISLTHTDQHYAQIIDVLAPQGQLALIDDPASLDAMPLKRKSISLHWE 264 Query: 233 GVFTLMPLLTGKGRAHHGEIMEQATRLIEAGKIVVQVDQRHFGFDQADA--AYDVIDAGG 290 +FT T H E++++ ++L++ G + + + D A+ A+ ++++G Sbjct: 265 LMFTRSMFQTPDMIRQH-ELLQRVSQLLDQGVLRTTTGEHYGRIDAANLRRAHGLLESGK 323 Query: 291 ARGKLVIE 298 A+GK+V+E Sbjct: 324 AKGKIVLE 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,011,658 Number of extensions: 45125 Number of successful extensions: 187 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 6 Length of query: 301 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 216 Effective length of database: 1,217,395 Effective search space: 262957320 Effective search space used: 262957320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)