BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0241.0022.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogen... 193 6e-51 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 80 7e-17 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 80 1e-16 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 68 5e-13 gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbas... 61 6e-11 >gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 328 Score = 193 bits (490), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 92/125 (73%), Positives = 103/125 (82%) Query: 44 FSTTDFVSVHLRLNDATRGIVTAQDLAQMKPTATFINTSRAELVASGALETALQQGRPGF 103 F+ D +S+HLRLNDATRGIV A DLA+MK TA F+NTSRAELV ALE AL GRPG Sbjct: 203 FAQADVLSLHLRLNDATRGIVKASDLARMKATACFVNTSRAELVEPDALEAALIAGRPGL 262 Query: 104 AGIDVFETEPLPHDSPLLRMDNVVATPHLGYVEKDGYELYFRAAFQNIVDFANGAPKNVL 163 A +DV+E EPLP +S LL+MDNVVA PHLGYVEKDGYELYFRAAFQNIV +A GAP +VL Sbjct: 263 AALDVYEREPLPVESALLQMDNVVAAPHLGYVEKDGYELYFRAAFQNIVAYAAGAPTHVL 322 Query: 164 NPEAL 168 NPEAL Sbjct: 323 NPEAL 327 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 80.5 bits (197), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 48/116 (41%), Positives = 60/116 (51%) Query: 44 FSTTDFVSVHLRLNDATRGIVTAQDLAQMKPTATFINTSRAELVASGALETALQQGRPGF 103 + DFV V L L T + A + A MK +A FIN SR +V AL ALQ Sbjct: 198 LAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRIVDEAALIAALQDKTIHG 257 Query: 104 AGIDVFETEPLPHDSPLLRMDNVVATPHLGYVEKDGYELYFRAAFQNIVDFANGAP 159 AG+DVFE EPLP SPLL++ NVVA PH+G + A N++ G P Sbjct: 258 AGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRLAMAELAVTNLIAGLRGEP 313 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 79.7 bits (195), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 46/122 (37%), Positives = 62/122 (50%), Gaps = 11/122 (9%) Query: 42 TPFSTTDFVSVHLRLNDATRGIVTAQDLAQMKPTATFINTSRAELVASGALETALQQGRP 101 T F+ +D VS+H L + ++ AQ LA+MK A +NT+R L+ AL AL G+ Sbjct: 193 TLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQALIAALDSGKL 252 Query: 102 GFAGIDVFETEPLPHDSPLLRMDNVVATPHLGYVEKDGYELYFRAAFQNIVDFANGAPKN 161 AG+D FE EP PL R+ N V +PH+G V D Y + GA N Sbjct: 253 RAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTSDAY-----------IAMGTGAASN 301 Query: 162 VL 163 VL Sbjct: 302 VL 303 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 67.8 bits (164), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 45/122 (36%), Positives = 62/122 (50%), Gaps = 2/122 (1%) Query: 48 DFVSVHLRLNDATRGIVTAQDLAQMKPTATFINTSRAELVASGALETALQQGRPGFAGID 107 D V + L + T + A +LA MKPTAT N +R +V AL AL++GR AG+D Sbjct: 204 DHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARGGIVDDAALIAALREGRIAAAGVD 263 Query: 108 VFETEPLPHDSPLLRMDNVVATPHLGYVEKDGYELYFRAAFQNIVDFANGA-PKNVLNPE 166 VFE EP L + NVV TPH+ A N++ +G P N+LNP+ Sbjct: 264 VFENEP-AFKPEFLDLSNVVLTPHIASASTPTRLAMANCAADNLIAALSGQRPPNLLNPD 322 Query: 167 AL 168 + Sbjct: 323 VV 324 >gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 60.8 bits (146), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 41/127 (32%), Positives = 61/127 (48%), Gaps = 13/127 (10%) Query: 44 FSTTDFVSVHLRLNDATRGIVTAQDLAQMKPTATFINTSRAELVASGALETALQQGRPGF 103 F+ +D +S+H L +ATR +V AQ LA KP +NTSR LV + A AL+ GR Sbjct: 195 FAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDTEAAIAALKSGRLRG 254 Query: 104 AGIDVFETEP----------LPHDSPLLRMD---NVVATPHLGYVEKDGYELYFRAAFQN 150 IDV+E E + D + R+ NV+ T H + ++ + +N Sbjct: 255 LAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFTEEAIGQIMQTTIEN 314 Query: 151 IVDFANG 157 + FA G Sbjct: 315 LSAFAAG 321 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 516,284 Number of extensions: 19282 Number of successful extensions: 89 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 8 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)