BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0240.0023.p (279 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 510 e-146 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 80 2e-16 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 78 9e-16 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 74 1e-14 gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 56 2e-09 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 510 bits (1314), Expect = e-146, Method: Compositional matrix adjust. Identities = 237/278 (85%), Positives = 257/278 (92%) Query: 2 CAVHAGDEVIVIEPAYDCYVPAIELAGGIPVFVQMTVSSDGYSVPWDKVSAAVTSKTRLI 61 CAVH GDEVIVIEP YDCYVPAIELAGG+PVFVQM V + GYS+PWDKV AAVT KTR+I Sbjct: 108 CAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGAQGYSIPWDKVKAAVTPKTRMI 167 Query: 62 MVNTPHNPTGSILKKADVNALADIVRGTDILILSDEVYEHMVYDGQAHESLCRHPELAPR 121 MVNTPHNPTGS+++ ADV ALADIVRGTDILILSDEVYEHMVYDGQ HESL RHPELA R Sbjct: 168 MVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYEHMVYDGQPHESLARHPELAER 227 Query: 122 TFINSSFGKTYHVTGWKIGFVAAPAALTAEFRKVHQFNVFTVNTPVQYGIAEYMKNPAPY 181 +FINSSFGKTYHVTGWK+G+VAAPAALTAEFRKVHQFNVFTVNTPVQYG+A YMK+PAPY Sbjct: 228 SFINSSFGKTYHVTGWKVGYVAAPAALTAEFRKVHQFNVFTVNTPVQYGLAAYMKDPAPY 287 Query: 182 VELPAFYQRKRDLFRDGLKNTRFKLLPSDGTYFQCVEYGAISDLSEAEFSKWLTTEIGVA 241 ++LPAFYQ KRDLFR GL NTRF+LLPS GTYFQCV+YGAIS L EAEF KWLT+EIGVA Sbjct: 288 LDLPAFYQHKRDLFRTGLANTRFELLPSQGTYFQCVKYGAISALPEAEFCKWLTSEIGVA 347 Query: 242 AIPVSAFYNTPKESGIVRFCFAKKDETLRLALDRLASL 279 AIPVSAFYNTP+ESGIVRFCFAKKDETL+LAL+RLA L Sbjct: 348 AIPVSAFYNTPRESGIVRFCFAKKDETLQLALERLAKL 385 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 68/281 (24%), Positives = 125/281 (44%), Gaps = 17/281 (6%) Query: 7 GDEVIVIEPAYDCYVPAIELAGGIPVFVQMTVSSDGYSVPWDKVSAAVTS---KTRLIMV 63 GD V+V P+Y ++ +AG V+M+ D + D++ AV K +++++ Sbjct: 153 GDTVLVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFF----DELERAVRESYPKPKMMIL 208 Query: 64 NTPHNPTGSILKKADVNALADIVRGTDILILSDEVYEHMVYDGQAHESLCRHP---ELAP 120 P NPT ++ + + R IL++ D Y + +DG S+ + P E+A Sbjct: 209 GFPSNPTAQCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPSIMQVPGAREVAV 268 Query: 121 RTFINSSFGKTYHVTGWKIGFVAAPAALTAEFRKVHQFNVFTVNTPVQY-GIAEYMKNPA 179 F + K+Y++ GW+IGF+ A L A ++ ++ + TPVQ IA + + Sbjct: 269 EFF---TLSKSYNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAAIAALEGDQS 325 Query: 180 PYVELPAFYQRKRDLFRDGLKNTRFKL-LPSDGTYFQCVEYGAISDLSEAEFSKWLTTEI 238 E+ A Y+R+R++ GL + + +P Y EF++ L + Sbjct: 326 CVEEIRANYERRRNVLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLEFARILLEQA 385 Query: 239 GVAAIPVSAFYNTPKESGIVRFCFAKKDETLRLALDRLASL 279 V P F E VRF + + +R A+ + ++ Sbjct: 386 KVCVSPGIGFGEYGDE--YVRFALIENESRIRQAIRGIKAM 424 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 77.8 bits (190), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 70/284 (24%), Positives = 130/284 (45%), Gaps = 26/284 (9%) Query: 4 VHAGDEVIVIEPAYDC---YVPAIE-----LAGGIPVFVQMTVSSDGYSVPWDKVSAAVT 55 V EV++ +P+Y C +V A E +A G Q++ V Sbjct: 114 VERDSEVLMPDPSYPCNRHFVAAFEGRAKLIASGPEHRFQLSA---------QMVREHWG 164 Query: 56 SKTRLIMVNTPHNPTGSILKKADVNALADIVRGTDILILSDEVYEHMVYDGQAHESLCRH 115 S TR +++ +P NPTG+ + ++ A+ VR + DE+Y+ + Y+G +L Sbjct: 165 SATRGVLLASPSNPTGTSILPDELRAIVGEVRQRGGFTIVDEIYQGLSYEGAPFSALS-- 222 Query: 116 PELAPRTFINSSFGKTYHVTGWKIGFVAAPAALTAEFRKVHQFNVFTVNTPVQYGIAEYM 175 L + +SF K +++TGW++G++ P AL + K+ Q + ++ Q+ Sbjct: 223 --LGDDVVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACF 280 Query: 176 --KNPAPYVELPAFYQRKRDLFRDGLKNTRFKL-LPSDGTYFQCVEYGAISDLSEAEFSK 232 + A Y E A ++R+RD L++ F + + DG ++ + A++D ++ + S Sbjct: 281 TPQTLALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAFYVYADCSALADDAD-QLSL 339 Query: 233 WLTTEIGVAAIPVSAFYNTPKESGIVRFCFAKKDETLRLALDRL 276 + E GV +P F +R +A E L+ A+ RL Sbjct: 340 DMLNEAGVVLVPGLDF-GPFTARRYIRLSYATSMENLQEAVARL 382 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 46/174 (26%), Positives = 81/174 (46%), Gaps = 9/174 (5%) Query: 4 VHAGDEVIVIEPAYDCYVPAIELAGGIPVFVQMTVSSDGYSVPWDKVSAAVTSKTRLIMV 63 ++ GDEV+V P Y + A+ L+GG PV + G+ + +T T+ I+V Sbjct: 116 LNTGDEVLVPSPDYPLWTAAVSLSGGTPVHY-VCDEQQGWQPDIADIKKKITPNTKAIVV 174 Query: 64 NTPHNPTGSILKKADVNALADIVRGTDILILSDEVYEHMVYDGQAHESLCRHPELAPRTF 123 P+NPTG++ + + ++ R ++IL+DE+Y+ ++YDG H SL + F Sbjct: 175 INPNNPTGALYSVEVLKEIIELARQHQLIILADEIYDKVLYDGNTHTSLASLADDV--LF 232 Query: 124 IN-SSFGKTYHVTGWKIGFVAAPAALTAEFRKVHQFNVFT-----VNTPVQYGI 171 I + K Y G++ G++ + N+ N P Q+ I Sbjct: 233 ITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRLCANAPGQFAI 286 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 64/258 (24%), Positives = 106/258 (41%), Gaps = 34/258 (13%) Query: 7 GDEVIVIEPAYDCYVPAIELAGGIPVFVQMTVSSDGYSVPWDKVSAAVTSKTRLIMVNTP 66 G V+ P Y Y + LAG P FV + ++ + +V V + +L+ V +P Sbjct: 134 GALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKRN-FAPDFSQVPQDVWERVQLLFVCSP 192 Query: 67 HNPTGSILKKADVNALADIVRGTDILILSDEVYEHMVYDGQAHESLCRHPELAPRTFIN- 125 NPTG++L D L + +I SDE Y + ++ + L R + Sbjct: 193 GNPTGAVLTLEDWKELFALSDRYGFVIASDECYSEIYFNEDPPLGGMQAARLLGRDYRRL 252 Query: 126 ---SSFGKTYHVTGWKIGFVAAPAALTAEF---RKVHQFNVFTV-----------NTPVQ 168 SS K +V G + GFVA AA+ +F R H + V T VQ Sbjct: 253 ISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQ 312 Query: 169 YGIAEYMKNPAPYVELPAFYQRKRDLFRDGLKNTRFKLLPSDGTYFQCVEYGAISDLSEA 228 IA+Y+ + + L ++ L+ LP D ++ + + ++S+ Sbjct: 313 GNIAKYVTK----------FSQVTPLLQEVLEVA----LP-DAAFYLWAKVDKLVNISDT 357 Query: 229 EFSKWLTTEIGVAAIPVS 246 EF++ L E V +P S Sbjct: 358 EFAQRLYAEYNVTVLPGS 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 879,236 Number of extensions: 35360 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 110 Number of HSP's successfully gapped: 5 Length of query: 279 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 195 Effective length of database: 1,222,130 Effective search space: 238315350 Effective search space used: 238315350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)