BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0240.0020.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 404 e-114 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 218 3e-58 gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 62 6e-11 gi|300311017|ref|YP_003775109.1| glutathione S-transferase famil... 61 8e-11 gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herb... 57 1e-09 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 404 bits (1039), Expect = e-114, Method: Compositional matrix adjust. Identities = 190/230 (82%), Positives = 209/230 (90%) Query: 1 MIDVYSWATPNGHKVHIMLEELGLDYRTHAIDIGAGDQFTDEFLKISPNNKIPAIVDSDG 60 MIDVYSWATPNGHKVHIMLEE GL Y HAIDIGAGDQFT EFLKISPNNKIPAIVD DG Sbjct: 1 MIDVYSWATPNGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDG 60 Query: 61 PDGQPMSLFESGAILIYLAGKTGKLLGATDREKYNVLQWVMFQMGGLGPMLGQAHHFRIY 120 PDG+P+SLFESGAILIYLAGKTG+ LG T REKY VLQWVMFQMGGLGPMLGQAHHFR+Y Sbjct: 61 PDGEPISLFESGAILIYLAGKTGRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLY 120 Query: 121 APEKIEYAVNRYSNEAKRLYGVLDKQLSRTAYLAGDAYTIADIASFPWTRSWKNQGIELS 180 APE+IEYAVNRY+NEA+RLY V+DKQL + AYLAG+ YTIADIA FPWTRS KNQGI+L Sbjct: 121 APEQIEYAVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLD 180 Query: 181 EYPNVQRWFDAISERPAVQRGVTVLADKRKALTDDKARDILFGNSQYKKR 230 ++PNV+RW+DAIS RPAVQRGVTVLADKRK L DDKA+++LFG SQY+KR Sbjct: 181 DFPNVKRWYDAISARPAVQRGVTVLADKRKPLHDDKAKELLFGKSQYQKR 230 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 218 bits (555), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 109/212 (51%), Positives = 143/212 (67%), Gaps = 7/212 (3%) Query: 2 IDVYSWATPNGHKVHIMLEELGLDYRTHAIDIGAGDQFTDEFLKISPNNKIPAIVDSDGP 61 I +YS TPNG KV IMLEE GL Y H + DQ + EFL ++PNNKIPAI+D +GP Sbjct: 22 IQLYSLPTPNGVKVSIMLEETGLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNGP 81 Query: 62 DGQPMSLFESGAILIYLAGKTGKLLGATDREKYNVLQWVMFQMGGLGPMLGQAHHFRIYA 121 GQP+ LFESGAIL+YLA KTG+ L A +Y V+QWVMFQMGG+GPM GQ F +A Sbjct: 82 GGQPLPLFESGAILLYLAEKTGQFLPADAAARYQVIQWVMFQMGGVGPMFGQLGFFHRFA 141 Query: 122 PEKIE--YAVNRYSNEAKRLYGVLDKQLSRTAYLAGDAYTIADIASFPWTRSW-----KN 174 ++ E +RY NE+KRL GV+DK+L+++ +L GDAY+IADIA+FPW R+ Sbjct: 142 GKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWIRNLIGFYEAA 201 Query: 175 QGIELSEYPNVQRWFDAISERPAVQRGVTVLA 206 + ++P+V R +A RPAV RG+ + A Sbjct: 202 DLVGFQDFPHVARALEAFVARPAVVRGLNIPA 233 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 61.6 bits (148), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 49/190 (25%), Positives = 81/190 (42%), Gaps = 15/190 (7%) Query: 11 NGHKVHIMLEELGLDYRTHAIDIGAGDQFTDEFLKISPNNKIPAIVDSDGPDGQPMSLFE 70 + H+ + LGL +D+ AG FL ++P ++P + D + + + + Sbjct: 11 HAHRAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGE------VVIAD 64 Query: 71 SGAILIYLAGKTGKL--LGATDREKYNVLQWVMFQMGGL--GPMLGQAHHFRIYAPEKIE 126 S AIL+YLA K G+ L + V +W+ G L GP R+ Sbjct: 65 SNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAA-----RLVTVFGAA 119 Query: 127 YAVNRYSNEAKRLYGVLDKQLSRTAYLAGDAYTIADIASFPWTRSWKNQGIELSEYPNVQ 186 + A L G L++ L++ +LA D TIAD+A + + ++L YP VQ Sbjct: 120 FNAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQ 179 Query: 187 RWFDAISERP 196 + I P Sbjct: 180 GFLQRIEALP 189 >gi|300311017|ref|YP_003775109.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 60.8 bits (146), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 59/232 (25%), Positives = 92/232 (39%), Gaps = 49/232 (21%) Query: 1 MIDVYSWATPNGHKVHIMLEELGLDYRTHAIDIGAGDQFTD-EFLKISPNNKIPAIVDSD 59 MI V+ +V +LEELGLDY E LK+ P K P I D + Sbjct: 1 MITVHHLNNSRSQRVLWLLEELGLDYEIKFYQRDPKTMLAPPELLKVHPLGKSPVITDGE 60 Query: 60 GPDGQPMSLFESGAILIYL----AGKTGKLLGATDREKYNVLQWVMFQMGGLGP------ 109 +++ ESG I+ YL +G L + E+ W+ F G P Sbjct: 61 ------LTVAESGNIIEYLLERYGAHSGLLPASGTPERLRYRYWLHFAEGSAMPWLVMQL 114 Query: 110 ------------------MLGQAHHFRIYAPEKIEYAVNRYSNEAKRLYGVLDKQLSRTA 151 ML + R+ AP++ +R ++ +L + Sbjct: 115 IFAKMPKAVPTLIRPIARMLAEGMRSRVVAPQQ------------ERQLRFMEDELGQQG 162 Query: 152 YLAGDAYTIADI-ASFPWTRSWKNQGIELSEYPNVQRWFDAISERPAVQRGV 202 + AG ++ ADI SFP + + +G YPN+ ++ +AI RPA QR + Sbjct: 163 WFAGAQFSGADIQMSFP-VEALRARGGLDGRYPNLLKYLEAIHARPAYQRAL 213 >gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 57.0 bits (136), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 57/197 (28%), Positives = 89/197 (45%), Gaps = 15/197 (7%) Query: 11 NGHKVHIMLEELGLDYRTHAIDIGAGDQFTDE--FLKISPNNKIPAIVDSDGPDGQPMSL 68 N KV ELG+ + D GAG + T E FL ++PN +P +++ DG L Sbjct: 12 NVRKVLWACAELGIAFERE--DWGAGFRPTSEPAFLAMNPNAMVP-VIEEDG-----FVL 63 Query: 69 FESGAILIYLAGKTG--KLLGATDREKYNVLQWVMFQMGGLGPMLGQAHHFRIYAPEKIE 126 +ES AI+ YLA + G L + + V QW+ +Q L A+ + Sbjct: 64 WESNAIIRYLANRHGGAALYPLQAQARARVDQWIDWQASDLNRAWSYAYLTLVRKSPAHG 123 Query: 127 YAVNRYSNEA--KRLYGVLDKQLSRTA-YLAGDAYTIADIASFPWTRSWKNQGIELSEYP 183 A ++ A R VL++QL + Y+AG +T+ADI+ W E P Sbjct: 124 NAQQVAASIADWTRFMEVLEQQLQHSGGYVAGAEFTLADISVGLSVNRWFGTPFEHPPLP 183 Query: 184 NVQRWFDAISERPAVQR 200 V ++D ++ER +R Sbjct: 184 AVAAYYDRLTERDGFRR 200 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 787,928 Number of extensions: 33914 Number of successful extensions: 106 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 6 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)