BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0238.0013.p (529 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein... 303 2e-83 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 93 5e-20 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 89 6e-19 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 87 4e-18 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 76 6e-15 >gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein [Herbaspirillum seropedicae SmR1] Length = 1047 Score = 303 bits (776), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 167/419 (39%), Positives = 251/419 (59%), Gaps = 17/419 (4%) Query: 74 LMLAVVMKKGFNGLTLGKSLTRERDLIQSSLPLGMSLTKVSDQALAIEAAIDEFMIKFVM 133 L + V M+ G + + LGK+L + ++ LP G++LT VS A+E ++D+F+ Sbjct: 287 LGIGVTMQPGGDVIRLGKALDARTEELRKRLPAGLTLTAVSSMPHAVEKSVDDFLEAVAE 346 Query: 134 ALAVVIVVSLVTLGFRVGVVVAAAVPLTLSAVFVIMLATGRDFDRITLGALILSLGLLVD 193 A+A+V+VVSLV+LG R G+VV ++P+ L+ + M +++LG L+L+LGLLVD Sbjct: 347 AVAIVLVVSLVSLGIRTGMVVVISIPIVLAVTSLFMYLFDIGLHKVSLGTLVLALGLLVD 406 Query: 194 DAIIAIEMMVVKMEEGMDRISAATYAWGATAAPMASGTLVTIIGFLPVGFARSTAGEYAG 253 DAIIA+EMM VK+E+G +R AA +A+ +TA PM +GTLVT+ GFLP+ A+S GEY Sbjct: 407 DAIIAVEMMAVKLEQGWNRRRAAAFAYTSTAFPMLTGTLVTVAGFLPIALAKSGTGEYTR 466 Query: 254 NIFWVVAFSLITSWFVAVLFIPYLGVKMLPDIP-----------VHAGGQDA-----IYA 297 +IF V A +L+ SW AV+ IP LG ML + P HAG + IY Sbjct: 467 SIFEVSAIALLLSWLAAVVLIPLLGYHMLKEKPGALTDEQIAANPHAGHDEEDHDHPIYQ 526 Query: 298 TRNYQRFRALVRWCVDRKWITAAVTIGMFAAAIFGMGLVQKQFFPNSERPELTIEVNLPP 357 TR Y R V WCV + T +F ++ LV +QFFP+S+RPEL I+++LP Sbjct: 527 TRIYTWLRRSVAWCVAFRGRVVIATTVLFIGSLAAFALVPQQFFPSSDRPELLIDLHLPE 586 Query: 358 GSAFSATEATVKKIEEALRKEPEAKIVSSYIGQGAPRFFLSVNPELPNPAFAQLIVLTDN 417 ++F AT+ ++E L+ P+ S++G GAPRF+L ++ +L +P FAQ +V + Sbjct: 587 RASFEATQREAVRMEALLKDRPQIDHYVSFVGVGAPRFYLPLDQQLASPNFAQFVVTAKS 646 Query: 418 ARDRDALKVRFRKMIDDGKFANARVRVSQFVFGPPVPFPVLFRVVGPDLNTVRDIAFKV 476 DR+AL + + +F+ R R+++ GPPV FPV FR+ G + VR IA +V Sbjct: 647 VEDREALARALEEPLRR-QFSGVRTRLTRLENGPPVGFPVQFRISGEQIPKVRAIAEQV 704 Score = 67.4 bits (163), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 28/71 (39%), Positives = 48/71 (67%) Query: 4 FNLSAFGVRERAVTLFLIIAIAAAGAFAFIKLGRAEDPSFTVKIMTVTAIWPGATAREMQ 63 FNLSA+ ++ +A+ F++ + G ++ +L ++EDP FT K+M + WPGATAR++Q Sbjct: 7 FNLSAWTLQHQALVTFILALVTLLGIGSYSRLSQSEDPPFTFKVMVIQTDWPGATARQVQ 66 Query: 64 QQVGDRLEXSL 74 +Q+ DR+ L Sbjct: 67 EQITDRIARKL 77 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 93.2 bits (230), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 75/314 (23%), Positives = 146/314 (46%), Gaps = 5/314 (1%) Query: 81 KKGFNGLTLGKSLTRERDLIQSSLPLGMSLTKVSDQALAIEAAIDEFMIKFVMALAVVIV 140 + G N + + S+ + +Q+SLP + + ++D+ I A++D+ + ++++A+V++ Sbjct: 288 QPGANVIAVVDSIKKILPQLQASLPGAIDVHILTDRTTTIRASVDDVKFELLLSVALVVM 347 Query: 141 VSLVTL-GFRVGVVVAAAVPLTLSAVFVIMLATGRDFDRITLGALILSLGLLVDDAIIAI 199 V V L ++ + AVPL+L F IM G + +TL AL ++ G +VDDAI+ I Sbjct: 348 VIFVFLRNVPATIIPSVAVPLSLVGTFAIMYLAGFSINNLTLMALTIATGFVVDDAIVMI 407 Query: 200 EMMVVKMEEGMDRISAATYAWGATAAPMASGTLVTIIGFLPVGFARSTAGEYAGNIFWVV 259 E + +EEGM + AA + S T I +P+ F G + Sbjct: 408 ENISRYIEEGMKPMQAALKGAEQIGFTIISLTFSLIAVLIPLLFMGDVVGRLFHEFAVTL 467 Query: 260 AFSLITSWFVAVLFIPYLGVKMLPDIPVHAGG----QDAIYATRNYQRFRALVRWCVDRK 315 A S++ S V++ P + ++L P G + + R R+ +++W + R+ Sbjct: 468 AVSILISAVVSLTLTPMMCARLLRHQPEEQQGWFYHKSQQFFDRIIARYGVMLQWVLARQ 527 Query: 316 WITAAVTIGMFAAAIFGMGLVQKQFFPNSERPELTIEVNLPPGSAFSATEATVKKIEEAL 375 +T V + V K FFP + + +FSA +++ + + Sbjct: 528 KLTLLVAAATLGLTVLLYVFVPKGFFPLQDTGVIQGISEATQSISFSAMGEKQQQLAKVV 587 Query: 376 RKEPEAKIVSSYIG 389 ++P + ++S+IG Sbjct: 588 LQDPAVESLTSFIG 601 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 89.4 bits (220), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 76/307 (24%), Positives = 141/307 (45%), Gaps = 9/307 (2%) Query: 76 LAVVMKKGFNGLTLGKSLTRERDLIQSSLPLGMSLTKVSDQALAIEAAIDEFMIKFVMAL 135 LA+ + G N L K++ + ++ P GM + K D + +I+E + + A+ Sbjct: 289 LAIKLATGANALDTVKAIDKRVAELEKFFPTGMKVQKPYDTTPFVRISIEEVVRTLIEAV 348 Query: 136 AVV-IVVSLVTLGFRVGVVVAAAVPLTLSAVFVIMLATGRDFDRITLGALILSLGLLVDD 194 +V +V+ L FR ++ AVP+ L F ++ G + +T+ A++L++GLLVDD Sbjct: 349 VLVFLVMYLFLQNFRATLIPTIAVPVVLLGTFGVLAVFGFTINTLTMFAMVLAIGLLVDD 408 Query: 195 AIIAIEMMV-VKMEEGMDRISAATYAWGATAAPMASGTLVTIIGFLPVGFARSTAGEYAG 253 AI+ +E + V EEG+ A + G + + LV F+P+ F + G Sbjct: 409 AIVVVENVERVMSEEGLPPKQATRKSMGQISGALVGVALVLAAVFVPMAFFSGSTGVIYR 468 Query: 254 NIFWVVAFSLITSWFVAVLFIPYLGVKML-PDIPVHAGGQDAIYATRNYQ------RFRA 306 + ++ S VA++ P L +L P HA + N Q R++ Sbjct: 469 QFSITIVSAMTLSVLVALVLTPALCATLLKPAAKGHALATTGFFGWFNRQFDRSNIRYQG 528 Query: 307 LVRWCVDRKWITAAVTIGMFAAAIFGMGLVQKQFFPNSERPELTIEVNLPPGSAFSATEA 366 +VR + R W A + A + G + F P+ ++ L + LP G+ + T+ Sbjct: 529 IVRHMLGRGWRYMAAYAVLLALVVAGFMKLPVGFLPDEDQGTLFAIMQLPSGATKARTDE 588 Query: 367 TVKKIEE 373 ++++E+ Sbjct: 589 VIRQVEQ 595 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 86.7 bits (213), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 99/409 (24%), Positives = 180/409 (44%), Gaps = 44/409 (10%) Query: 6 LSAFGVRERAVTLFLIIAIAAAGAFAFIKLGRAEDPSFTVKIMTVTAIWPGATAREMQQQ 65 L + G ++ A T+ + + + F I L RA TV++ V + G + + + Sbjct: 222 LPSPGTQQIAATVVVNGQLTSPEQFGNIIL-RANANGATVRLRDVARVEVGGQSYDTSGR 280 Query: 66 VGDRLEXSLMLAVVMKKGFNGLTLGKSLTRERDLIQSSLPLGMSLTKVSDQALAIEAAID 125 + + S + V + N L ++ ++ + + P G+ T D + ++ +I+ Sbjct: 281 LDGK--PSSFIGVQLSPTANALGTATAVHKKMEELAKYFPTGVKYTIPYDTSKFVKISIE 338 Query: 126 EFMIKFVMALAVVIVVSLVTL-GFRVGVVVAAAVPLTLSAVFVIMLATGRDFDRITLGAL 184 E + + A+ +V +V + L R ++ VP+ L F +L G + +T+ + Sbjct: 339 EVVKTLIEAIVLVFLVMFLFLQNIRYTIIPTIVVPVALLGTFATLLLFGFSINVLTMFGM 398 Query: 185 ILSLGLLVDDAIIAIEMMVVKM-EEGMDRISAATYAWGATAAPMASGTLVTIIGFLPVGF 243 +L++G+LVDDAI+ +E + M EEG+ A A G + + TLV I F+P+ F Sbjct: 399 VLAIGILVDDAIVVVENVERIMSEEGLSPADATRKAMGQISGALIGITLVLIAVFIPMAF 458 Query: 244 ARSTAGEYAGNIFWVVAFSLITSWFVAVL----FIPYLGVKMLPDIPVHAGGQDAIYATR 299 G G I+ + S++ S +VL P L +L PV G Sbjct: 459 ----FGGAVGAIYRQFSLSMVASMVFSVLMAFTLTPALCATILK--PVEKG--------- 503 Query: 300 NYQRFRALVRWCVDRKWITAA-----VTIGMFAAA----------IFGMGLVQ----KQF 340 ++ + W +RK+ + A + GM A + +GL+ F Sbjct: 504 HHHEKKGFFGW-FNRKFNSTASGYQGIIGGMLNKAGRYMIVYVLIVACVGLLYVRLPASF 562 Query: 341 FPNSERPELTIEVNLPPGSAFSATEATVKKIEEALRKEPEAKIVSSYIG 389 PN ++ + V LPPG++ S T A VK+IEE K+P + + +G Sbjct: 563 LPNEDQGYIITNVQLPPGASTSRTTAVVKQIEEYYLKDPAVEHAIAVLG 611 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 76.3 bits (186), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 73/318 (22%), Positives = 146/318 (45%), Gaps = 19/318 (5%) Query: 74 LMLAVVMKKGFNGLTLGKSLTRERDLIQSSLPLGMSLTKVSDQALAIEAAIDEFMIKFVM 133 ++L+++ G + L + +L + +LP + +T + DQ++ ++AA+ + + ++ Sbjct: 281 VLLSILKNGGASTLDIVDNLRKLLPTAAQTLPEDIKITPLFDQSVFVKAAVTGVISEALI 340 Query: 134 ALAVVIVVSLVTLG-FRVGVVVAAAVPLTLSAVFVIMLATGRDFDRITLGALILSLGLLV 192 A + + L+ LG +R +++ +PL++ A +I+ A G + +TLG L LS+G+LV Sbjct: 341 AAGLTAAMVLLFLGNWRSTLIIGLTIPLSIMAAILILAALGETLNLMTLGGLALSVGILV 400 Query: 193 DDAIIAIEMMVVKMEEGMDRISAATYAWGATAAPMASGTLVTIIGFLPVGFARSTAGEYA 252 D AI+ IE + + G A G P TL I F+P+ F A Sbjct: 401 DQAIVTIENIERHLHLGKPLHDAIIVGAGEIGVPAFVATLCICIVFVPMFFLSGVARFLF 460 Query: 253 GNIFWVVAFSLITSWFVAVLFIPYLGVKMLPDIPVHAGGQDAIYATRNYQRFRAL-VRWC 311 + V F++ S+ ++ +P L + ++ G D+ + + +RA R+ Sbjct: 461 VPLAEAVVFAMAASYLLSRTLVPTLVMLLMGS----HGQVDSAKQSLLQRLYRAFDNRFE 516 Query: 312 VDRKWITAAVTIGM-----FAAAIFGMGLVQ--------KQFFPNSERPELTIEVNLPPG 358 R T ++ + FA A G L+ + FFPN + ++ + + +P G Sbjct: 517 RVRHGYTLVLSSLLAHRRRFALAFLGFCLLSCLLYPFLGRDFFPNVDGGQIRLHMRMPTG 576 Query: 359 SAFSATEATVKKIEEALR 376 + T ++E +R Sbjct: 577 TRIEETARAADEVERVIR 594 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,209 Number of extensions: 48297 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 12 Length of query: 529 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 439 Effective length of database: 1,193,720 Effective search space: 524043080 Effective search space used: 524043080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)