BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0238.0012.p (238 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein... 196 2e-51 gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 75 5e-15 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 67 2e-12 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 66 3e-12 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 65 4e-12 >gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein [Herbaspirillum seropedicae SmR1] Length = 1047 Score = 196 bits (497), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 116/226 (51%), Positives = 159/226 (70%) Query: 5 NRELYIAVQGDVRDGVQPPDVTHAILPKLDAIKAALPAGYHIDTGGSVEESAKADVALAA 64 NR+ I V DVR GVQ DVT+ + KL+A++A LP GY ID GG VEE+AKA +++A Sbjct: 821 NRQPTITVLADVRPGVQGIDVTNDVNQKLNALRAELPVGYRIDVGGPVEENAKAQSSISA 880 Query: 65 MFPLMIILMLAVIMFQVRSFATMWMVFATAPLGLVGAVPTLLIFHQPFGFNAILGLIGLA 124 PLM+I +L ++M Q++SF MV TAPLGL+G V LL+F QPFGF A+LG I + Sbjct: 881 QMPLMVIAVLLLLMVQLQSFGRTMMVVMTAPLGLIGVVGALLLFGQPFGFVAMLGTIAML 940 Query: 125 GILIRNTLILVDQIRSDLLSGMTPYQAVIESTVRRARPVILTAVAAMLAFIPLTHSSFWG 184 GI++RN++ILVDQI D+ +G + A++ +TVRR RP+ LTA AA+LA IPL S+F+G Sbjct: 941 GIIMRNSVILVDQIEQDIAAGRGRFDAIVGATVRRFRPITLTAAAAVLALIPLLRSNFFG 1000 Query: 185 PLAYVLIGGVGVGTVLTLLFLPALYAIWFKVKRPTAPGRPTGAGHS 230 P+A L+GG+ + T+LTL +LPALYA+ ++VK R T A S Sbjct: 1001 PMATALMGGITIATILTLFYLPALYALCYRVKPDETHHRDTAAQGS 1046 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 75.1 bits (183), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 58/199 (29%), Positives = 94/199 (47%), Gaps = 7/199 (3%) Query: 1 LKRYNRELYIAVQGDVRDGVQPPDVTHAILPKLDAIKAALPAGYHIDTGGSVEESAKADV 60 + R + + I V +VR V A A+K +PAGY GG E A Sbjct: 823 ISREDGKRRIVVSANVRGRDLGGFVAEAERQLQSAVK--VPAGYWTTWGGQFENLQSATQ 880 Query: 61 ALAAMFPLMIILMLAVIMFQVRSFATMWMVFATAPLGLVGAVPTLLIFHQPFGFNAILGL 120 L + P+ ++++ ++ + +VF P L G + L + P +A +G Sbjct: 881 RLQIVIPVALLMVFVLLFAMFSNVKDGLLVFTGIPFALTGGILALAMRGIPMSISAAVGF 940 Query: 121 IGLAGILIRNTLILVDQIRSDLLSGMTPYQAVIESTVRRARPVILTAVAAMLAFIPL--- 177 I L+G+ + N L+++ IRS G T A+ E + R RPV++TA+ A L F+P+ Sbjct: 941 IALSGVAVLNGLVMISFIRSLREEGRTLDAAIHEGAMTRLRPVLMTALVASLGFVPMALA 1000 Query: 178 --THSSFWGPLAYVLIGGV 194 T + PLA V+IGG+ Sbjct: 1001 TGTGAEVQRPLATVVIGGI 1019 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 60/236 (25%), Positives = 112/236 (47%), Gaps = 11/236 (4%) Query: 1 LKRYNRELYIAVQGDVRDGVQPPDVTHAILPKLDAIKAALPAGYHID-TGGSVEESAKAD 59 L RYN + + G G P T + ++++ A LP G+ + TG S+EE + Sbjct: 811 LIRYNGYPAMKIAG----GAAPGRSTGEAMAEMESFVAKLPPGFGFEWTGQSLEEKTSGN 866 Query: 60 VALAAMFPLMIILMLAVIMFQVRSFATMWMVFATAPLGLVGAVPTLLIFHQPFGFNAILG 119 A A++ L +I + V+ S + V PLG++GA+ + + P +G Sbjct: 867 QA-PALYALSLIAVFLVLAALYESTSIPLAVMLVVPLGILGALLGVTLRAMPNDVYFKVG 925 Query: 120 LIGLAGILIRNTLILVDQIRSDLLSGMTPYQAVIESTVRRARPVILTAVAAMLAFIPLTH 179 +I + G+ +N +++++ + G +A +E+ R RP+I+T++A +L +PL Sbjct: 926 MIAVVGLSAKNAILIIEFAKDLQAQGKGLIEATLEAVHLRFRPIIMTSLAFILGVLPLAI 985 Query: 180 SSFWGPLAYVLI-----GGVGVGTVLTLLFLPALYAIWFKVKRPTAPGRPTGAGHS 230 +S G + I GG+ TVL + +P + + K+ + + R A HS Sbjct: 986 ASGAGSASQRAIGTGVMGGMITATVLAVFLVPIFFVVVRKIFKGSERQRKFDAEHS 1041 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 65.9 bits (159), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 42/126 (33%), Positives = 71/126 (56%), Gaps = 6/126 (4%) Query: 90 VFATAPLGLVGAVPTLLIFHQPFGFNAILGLIGLAGILIRNTLILVD-QIRSDLLSGMTP 148 + +T P VGA+ L++ F A++G+I L GI+ +N +++VD I ++ GM+P Sbjct: 928 IISTLPSAGVGALLALMVTGTDFSLIALIGVILLIGIVKKNAIMMVDFAIDAERNRGMSP 987 Query: 149 YQAVIESTVRRARPVILTAVAAMLAFIPL-----THSSFWGPLAYVLIGGVGVGTVLTLL 203 A+ E+ R RP+++T +AA+L IPL + PL ++GG+ V +LTL Sbjct: 988 RDAIFEACKLRFRPIMMTTMAALLGAIPLAIGHGDGAELRQPLGISIVGGLVVSQLLTLY 1047 Query: 204 FLPALY 209 P +Y Sbjct: 1048 TTPVVY 1053 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 65.5 bits (158), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 60/219 (27%), Positives = 110/219 (50%), Gaps = 15/219 (6%) Query: 1 LKRYNRELYIAVQGDVRDGVQPPDVTHAILPKLDAIKAALPAGYHID-TGGSVEESAKAD 59 L+RY + + G GV + + +++ + LP G+ + TG S++E A Sbjct: 809 LQRYQGFPAVRISGSAAPGV----ASGEAMAEMERLAQQLPPGFAVAWTGQSLQERLSAA 864 Query: 60 VA--LAAMFPLMIILMLAVIMFQVRSFATMWMVFATAPLGLVGAVPTLLIFHQPFGFNAI 117 A L A+ L++ L+LA + S++ V PLGLVGAV +++ P Sbjct: 865 QAPMLMALSMLVVFLVLAALY---ESWSIPLSVMLVVPLGLVGAVAAVILRDMPNDVFFK 921 Query: 118 LGLIGLAGILIRNTLILVDQIRSDLLSGMTPYQAVIESTVRRARPVILTAVAAMLAFIPL 177 +G+I + G+ +N +++V+ R GM +A I+++V R RP+++T++A L +PL Sbjct: 922 VGMITVIGLSAKNAILIVEFARQLRSEGMGLVEAAIKASVLRLRPILMTSLAFALGVVPL 981 Query: 178 THSS-----FWGPLAYVLIGGVGVGTVLTLLFLPALYAI 211 +S + + GG+ TVL +LF+P + + Sbjct: 982 MLASGASAETQRAIGTGVFGGMVSATVLAVLFVPVFFVL 1020 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 650,661 Number of extensions: 23145 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 159 Number of HSP's successfully gapped: 12 Length of query: 238 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 156 Effective length of database: 1,231,600 Effective search space: 192129600 Effective search space used: 192129600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)