BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0238.0011.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 328 4e-91 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 295 4e-81 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 280 1e-76 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 243 1e-65 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 159 3e-40 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 328 bits (841), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 190/376 (50%), Positives = 243/376 (64%), Gaps = 5/376 (1%) Query: 2 YNIQAADRLPNLALNGNGARQRLPADVNPTGRAGVNSSYFAPPPPPPPPPPPFGRVRNLS 61 Y I AD LPNL L+GN AR R A + TG+ +++SY P FGRVRNL Sbjct: 99 YQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLK 158 Query: 62 EAALQRYFATEQAQRAAQISLIAEVANTYEALVTDRRLLQLTESTLASRADSYDRQKQLY 121 +AAL Y +TE+AQ++AQISL+A+VA Y A + L+L + TL SR + D ++ Sbjct: 159 DAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRL 218 Query: 122 AEGASSEYDLRQAESLFEAAKAARAQQERRKTLDENALTLLVGHSLASDTGTPTAA--SA 179 GAS+ D R ++L E+A+ A+ Q ER++ ENALTLLVG + P A S Sbjct: 219 DVGASTALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGE---LPPAQLLSD 275 Query: 180 ELVLPDLPAGLPSDLLTARPDILQQEAVLRSQNASIGAARAAFFPRISLTGNFGSASSQL 239 E ++ D+PAGLPSDLLT RPDI E L + NA+IGAARAAFFPRISLTGNFGSASS L Sbjct: 276 EKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTL 335 Query: 240 SGLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAETDKKIAVAQYEKTVQVAFREVADAL 299 GLF+SGS +WSF P +S+PIF+ GRN ANL++AE K AV YEK +Q AFREVADAL Sbjct: 336 GGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADAL 395 Query: 300 AGRATLADEAGAVKAQVAAESARYGFAKSRYEAGYSSYLEYLDAQRSLFAIQQQSIQVQL 359 R +L ++ A +A +AA+ R R++ G +S L+ LDAQR LF+ QQ IQ + Sbjct: 396 VARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQ 455 Query: 360 AELQNRITLYKVLGGG 375 L N I LYK LGGG Sbjct: 456 LRLNNAIDLYKALGGG 471 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 295 bits (755), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 172/379 (45%), Positives = 236/379 (62%), Gaps = 4/379 (1%) Query: 2 YNIQAADRLPNLALNGNGARQRLPADVNPTGRAGVNSSYFAPPPPPPPPPPPFGRVRNLS 61 Y +Q A P++ + R P D++ TG A + SY A FGRVR+L Sbjct: 103 YGVQRAALFPSIKAATSETASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLK 162 Query: 62 EAALQRYFATEQAQRAAQISLIAEVANTYEALVTDRRLLQLTESTLASRADSYDRQKQLY 121 E ALQ + +T +A+R+ QISLIAEVA + L +D+ L+L + TL S++ SY ++ + Sbjct: 163 EQALQSFLSTAEARRSTQISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSF 222 Query: 122 AEGASSEYDLRQAESLFEAAKAARAQQERRKTLDENALTLLVGHSLASDT---GTP-TAA 177 G+SS LRQA++ E+A+ + D NAL LLVG L + G P T Sbjct: 223 ELGSSSALTLRQAQTSVESARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRL 282 Query: 178 SAELVLPDLPAGLPSDLLTARPDILQQEAVLRSQNASIGAARAAFFPRISLTGNFGSASS 237 +A L +P GLPSDLL R DI+Q E LR+ NA+IGAARAAF+P I+LT N G+AS+ Sbjct: 283 AAASPLATIPPGLPSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASA 342 Query: 238 QLSGLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAETDKKIAVAQYEKTVQVAFREVAD 297 +L+GLF GS SWSF+PQIS+PIF+GG N ANL++A + I+VAQYEK +Q AFREV+D Sbjct: 343 RLAGLFKGGSGSWSFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSD 402 Query: 298 ALAGRATLADEAGAVKAQVAAESARYGFAKSRYEAGYSSYLEYLDAQRSLFAIQQQSIQV 357 ALA R TL + A +A V A + +++RY +G SYL LD+QR+L++ Q+ I Sbjct: 403 ALAQRNTLGRQLQAQEALVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGT 462 Query: 358 QLAELQNRITLYKVLGGGW 376 QL+ N +T YK LGGGW Sbjct: 463 QLSRWTNLVTFYKTLGGGW 481 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 280 bits (717), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 170/383 (44%), Positives = 226/383 (59%), Gaps = 6/383 (1%) Query: 2 YNIQAADRLPNLALNGNGARQRLPADVNPTGRAGVNSSYFAPPPPPPPPPPPFGRVRNLS 61 Y I A+R P+++ G + R PA+++ TG A V+ FGR++NLS Sbjct: 110 YRITDANRYPSISATGTSSASRTPAELSTTGSAAVSHQQSVTVGVSAYELDFFGRLKNLS 169 Query: 62 EAALQRYFATEQAQRAAQISLIAEVANTYEALVTDRRLLQLTESTLASRADSYDRQKQLY 121 +AAL+ Y + +R+ ISL+AEVA + L +DR LL+L + T AS++ ++ ++ + Sbjct: 170 DAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSH 229 Query: 122 AEGASSEYDLRQAESLFEAAKAARAQQERRKTLDENALTLLVGHSLAS---DTGTPTAAS 178 G S DL E+ E+A+A A+ + D NAL LLVG + + G P AS Sbjct: 230 DIGTVSGVDLASQEATVESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDTAS 289 Query: 179 AELVLPDLPAGLPSDLLTARPDILQQEAVLRSQNASIGAARAAFFPRISLTGNFGSASSQ 238 VLP PAGLPS +L RPD+L E L+S NA IGAARAAFFP ISLT N G+AS Sbjct: 290 ---VLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRT 346 Query: 239 LSGLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAETDKKIAVAQYEKTVQVAFREVADA 298 L GLF +GS SW+F P I++PIF G A+L+ A+ + I VA YEK +Q AFREVADA Sbjct: 347 LGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADA 406 Query: 299 LAGRATLADEAGAVKAQVAAESARYGFAKSRYEAGYSSYLEYLDAQRSLFAIQQQSIQVQ 358 LA RATL A AA + + +RY G SYL L AQR+L++ QQ I ++ Sbjct: 407 LAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLR 466 Query: 359 LAELQNRITLYKVLGGGWKAAKQ 381 L E NRITLYKVLGGGW Q Sbjct: 467 LTEQSNRITLYKVLGGGWSEQTQ 489 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 243 bits (621), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 156/374 (41%), Positives = 219/374 (58%), Gaps = 1/374 (0%) Query: 1 MYNIQAADRLPNLALNGNGARQRLPADVNPTGRAGVNSSYFAPPPPPPPPPPPFGRVRNL 60 +Y I AD P + R R PAD+N T R V S+Y A +GR+R+L Sbjct: 102 LYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSL 161 Query: 61 SEAALQRYFATEQAQRAAQISLIAEVANTYEALVTDRRLLQLTESTLASRADSYDRQKQL 120 +AAL+ A++ + A +SL+A+VA+ + L L++ T+ASR +S+ + Sbjct: 162 DDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRR 221 Query: 121 YAEGASSEYDLRQAESLFEAAKAARAQQERRKTLDENALTLLVGHSLASDTGTPTAASAE 180 GA+S +L Q E+L A+A AQ ++++ NALTLLVG + G A Sbjct: 222 VEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSPVVLPVGQARAGLDA 281 Query: 181 LVLPDLPAGLPSDLLTARPDILQQEAVLRSQNASIGAARAAFFPRISLTGNFGSASSQLS 240 + P P GLP+ LLT RPD+ E L + NA+IGAARAAFFP ISLTGN G+AS++LS Sbjct: 282 VFAPLQP-GLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELS 340 Query: 241 GLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAETDKKIAVAQYEKTVQVAFREVADALA 300 GLF GS +W+F P I++PIF GR NL ++E + +AVA YEKTVQ AFREVADALA Sbjct: 341 GLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALA 400 Query: 301 GRATLADEAGAVKAQVAAESARYGFAKSRYEAGYSSYLEYLDAQRSLFAIQQQSIQVQLA 360 R L + ++ V + R + RY+ G + +L+ LDAQR L + QQQ +Q++ A Sbjct: 401 ARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRA 460 Query: 361 ELQNRITLYKVLGG 374 L + + LY LGG Sbjct: 461 LLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 159 bits (403), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 129/378 (34%), Positives = 197/378 (52%), Gaps = 3/378 (0%) Query: 2 YNIQAADRLPNLALNGNGARQRLPADVNPTGRAGVNSSYFAPPPPPPPPPPPFGRVRNLS 61 + IQ + LP + L R R P + +G A Y +GR+ +L+ Sbjct: 96 WRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAASTREYRVGLGASSWELDFWGRIASLN 155 Query: 62 EAALQRYFATEQAQRAAQISLIAEVANTYEALVTDRRLLQLTESTLASRADSYDRQKQLY 121 EAALQ + A+ A++A ISLI +VA + +L L L E+ A+ A+S + + Sbjct: 156 EAALQEWMASRAAEQALVISLIGQVAAAWTSLRELDERLHLLETLQAATAESLALMQARH 215 Query: 122 AEGASSEYDLRQAESLFEAAKAARAQQERRKTLDENALTLLVGHSLASDTGTPTAASAEL 181 G + L Q S +A +A+ ++ + + L +L G + P A + Sbjct: 216 DAGRADAAALAQQRSELAQMRAEQAELQQMRASQRSTLEMLAGLTQLPAALEP--APTTI 273 Query: 182 VLP-DLPAGLPSDLLTARPDILQQEAVLRSQNASIGAARAAFFPRISLTGNFGSASSQLS 240 +LP L GLPS LL ARPDI+ E L++ A + AARAAFFPRISLT G AS+ L Sbjct: 274 LLPFALAPGLPSTLLAARPDIIAAEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQ 333 Query: 241 GLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAETDKKIAVAQYEKTVQVAFREVADALA 300 LF SR+W+F+P+IS+P+ +GG + ++A D++ A+ YE+TVQ AFREVA L Sbjct: 334 DLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLE 393 Query: 301 GRATLADEAGAVKAQVAAESARYGFAKSRYEAGYSSYLEYLDAQRSLFAIQQQSIQVQLA 360 L ++ + V ++ R R G +S L+ L+++R QQ+ ++ A Sbjct: 394 EAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHA 453 Query: 361 ELQNRITLYKVLGGGWKA 378 L+N+I LY LGGG +A Sbjct: 454 LLRNQIALYVALGGGTQA 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.128 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 997,329 Number of extensions: 36363 Number of successful extensions: 171 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 17 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)