BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0020.p (171 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312746|ref|YP_003776838.1| dihydrofolate reductase oxidore... 253 8e-69 >gi|300312746|ref|YP_003776838.1| dihydrofolate reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 164 Score = 253 bits (645), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 128/166 (77%), Positives = 140/166 (84%), Gaps = 4/166 (2%) Query: 6 SASGALCVIVATDSQNGIGINNTLPWHLPEDLAHFKRTTSGHPIIMGRKTFDSIGRPLPN 65 ++SG L +IVATD NGIGINNTLPW LPEDLAHFKRTTSGH I+MGRKTFDSIGRPLPN Sbjct: 3 TSSGQLTLIVATDLNNGIGINNTLPWRLPEDLAHFKRTTSGHAILMGRKTFDSIGRPLPN 62 Query: 66 RRNIVITRNRDWSHAGVETVSSLQEAAVLVAGADAFIIGGAQIYAESLPLATRLIVTEIA 125 RRNIVITRN W H GVET SLQEAA L AGA AFIIGGAQIYAE+LPLA RLI+T IA Sbjct: 63 RRNIVITRNAGWQHDGVETAGSLQEAAALCAGAPAFIIGGAQIYAEALPLADRLIITRIA 122 Query: 126 QAFDCDAFFPAIDSKVWRETARETRHAESQGVAQGFDYAFVTYERR 171 Q FDCDAFFPA+D +VW+ETARE H+E+ G+ DYAFVTYERR Sbjct: 123 QTFDCDAFFPALDQQVWQETAREEHHSETSGL----DYAFVTYERR 164 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 549,864 Number of extensions: 21772 Number of successful extensions: 60 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 59 Number of HSP's successfully gapped: 1 Length of query: 171 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 92 Effective length of database: 1,245,805 Effective search space: 114614060 Effective search space used: 114614060 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)