BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0010.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312740|ref|YP_003776832.1| thymidylate synthetase [Herbasp... 137 9e-34 >gi|300312740|ref|YP_003776832.1| thymidylate synthetase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 137 bits (345), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 82/222 (36%), Positives = 114/222 (51%), Gaps = 48/222 (21%) Query: 6 DRTGTGTRSVFGHQMRFNLQEGFPLLTTKKLHIKSIVHELIWFLAGSTNIAYLKDNGVKI 65 +RTG T S+ G MRF+LQ+GFP +TTK+L KS+V EL FL + + A + G K+ Sbjct: 20 NRTGIRTLSIPGAMMRFDLQKGFPAVTTKRLAFKSVVGELCGFLRATRSAADFRALGCKV 79 Query: 66 WDEWADAEG------------NLGPIYGYQWRSWPA---PNGEH---------------- 94 WD+ A+ +LGP+YG QWR WPA + H Sbjct: 80 WDQNANENADWLANPFRSGADDLGPVYGVQWRQWPAYKLIDAAHTTQIEAARSKGFAIVS 139 Query: 95 ---------------IDQIAQVVSQIRNNPDSRRLIVSAWNVADIPQMKLPPCHAFFQFY 139 +DQ+ + + I NNP SRR++ WN A + ++ LP CH +QF Sbjct: 140 SLQDEGVEKVLLYKAVDQLRECLDTIVNNPGSRRILFHGWNCAVLDEVALPACHLLYQFL 199 Query: 140 --VADGKLSCQLYQRSADIFLGVPFNIASYALLTHMVAQQTG 179 + G++S LY RS DI LG PFN+A A L H+VA+ TG Sbjct: 200 PNQSTGEISLCLYVRSNDIGLGTPFNLAEGAALLHLVARLTG 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 857,211 Number of extensions: 34132 Number of successful extensions: 87 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 84 Number of HSP's successfully gapped: 1 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)