BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0235.0001.p (463 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 434 e-123 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 130 3e-31 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 114 1e-26 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 105 5e-24 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 78 1e-15 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 434 bits (1116), Expect = e-123, Method: Compositional matrix adjust. Identities = 212/335 (63%), Positives = 258/335 (77%), Gaps = 2/335 (0%) Query: 126 RQGARCELQLXGRSGLKLPLLSLGMWHNFGDTTSLATQREMLRTAFDLGITHFDLANNYG 185 R R + GRSGL LP +SLG+W NFG + T R +LR AFD G+THFDLANNYG Sbjct: 9 RYDGRMPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYG 68 Query: 186 PPYGSAEANFGHLFKQDFLPYRDELIISTKAGWDMWPGPYGQGGGSRKYVLASLDQSLKR 245 PP GSAE NFG +DFLPYRDEL+IS+KAGW MWPGPYG G+RK+++AS +QSLKR Sbjct: 69 PPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIASCEQSLKR 128 Query: 246 MNLDYVDIFYSHRFDPETPLEETMGALATAVQQGKALYVGVSSYSPEKTKEAAELLKQWK 305 M LDYVDIFYSHR D +TPLEETMGALA +QGKALYVG+SSYSPE T++AA +LK Sbjct: 129 MGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEG 188 Query: 306 VPCLIHQPSYNMFNRWIEQGLLDELERQGMGCITFTALAQGLLSDKYLNGIPEDARINRA 365 VP LIHQP+Y+M NR IE GLL LE G+GCI F+ LAQGLL+ KYL G+PE AR+ RA Sbjct: 189 VPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGARVTRA 248 Query: 366 GGGSLKSDHLSEENLQRVRALNDIAKARGQTLAQMALAWVLRDPRVTTTLIGASSSKQIR 425 SL LSE+NL R+RALN+IA RGQTLAQMA+AWVLRD RVT+ LIGA + +Q+ Sbjct: 249 --PSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLD 306 Query: 426 ENVAALDKLGFSADELAAIDKQAQDGHLNLWEVSS 460 +++ A++KL F+ +ELA+IDK A DG ++LW+VSS Sbjct: 307 DSLDAVNKLDFTQEELASIDKHATDGDVDLWKVSS 341 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 99/319 (31%), Positives = 157/319 (49%), Gaps = 13/319 (4%) Query: 137 GRSGLKLPLLSLGMWHNFGDTTSLATQREMLRTAFDLGITHFDLANNYGPPYGSAEANFG 196 G+S LK+ L LG FGD T L ++ +A + G+ D A+ Y G +E G Sbjct: 7 GKSNLKVSALCLGSMM-FGDQTDLQESGRIVASAREHGVNFIDTADVYTK--GKSEEIVG 63 Query: 197 HLFKQDFLPYRDELIISTKAGWDMWPGPYGQGGGSRKYVLASLDQSLKRMNLDYVDIFYS 256 L R E ++++K G M P + SR +++ + L R++ DY+DI Y Sbjct: 64 KLLAGQ----RHEWVLASKLGNAMSGKP-NESNYSRSWIVRETENILTRLDTDYLDILYM 118 Query: 257 HRFDPETPLEETMGALATAVQQGKALYVGVSSYSPEKTKEAAELLKQWKVPC-LIHQPSY 315 HR + LEE + AL ++ GK GVS++ + E L ++ VP L+ QP Y Sbjct: 119 HRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYY 178 Query: 316 NMFNRWIEQGLLDELERQGMGCITFTALAQGLLSDKYLNGIPEDARINRAGGGS--LKSD 373 N+ NR E +L G+G + ++ +A+G+L+ KY G + A+ RAG G + Sbjct: 179 NLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPG-QKPAQDTRAGRGDKRMMET 237 Query: 374 HLSEENLQRVRALNDIAKARGQTLAQMALAWVLRDPRVTTTLIGASSSKQIRENVAALDK 433 EE+L + L +ARG L Q A AWVL + +++ + G + Q+ + A + Sbjct: 238 EFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAE- 296 Query: 434 LGFSADELAAIDKQAQDGH 452 L SA+E A +D GH Sbjct: 297 LKISAEEEALVDSLVTPGH 315 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 114 bits (286), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 93/296 (31%), Positives = 148/296 (50%), Gaps = 27/296 (9%) Query: 156 DTTSLATQREMLRTAFDLGITHFDLANNYGPPYGSAEANFGHLFKQDFLPYRDELIISTK 215 D SLAT + A +LGI D A+ YGP Y + E L + RD+ I+TK Sbjct: 46 DAESLAT----IDRALELGINLLDTADMYGP-YTNEE-----LLGRAIKGRRDKFFIATK 95 Query: 216 AGWDMWPGPYGQGG--GSRKYVLASLDQSLKRMNLDYVDIFYSHRFDPETPLEETMGALA 273 G P G G GS Y+ +++ SLKR+ ++ +D++Y HR DP TP+E TMG LA Sbjct: 96 FGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDLYYQHRIDPATPIEVTMGVLA 155 Query: 274 TAVQQGKALYVGVSSYSPEKTKEAAELLKQWKV-PCLIHQPSYNMFNRWIEQGLLDELER 332 VQ GK Y+G+S S AA L + +V P Q Y+++ R E +L Sbjct: 156 DLVQAGKIRYIGLSEAS------AATLERAHRVHPVTALQSEYSLWTRDPEAEVLAACRT 209 Query: 333 QGMGCITFTALAQGLLSD--KYLNGIPEDARINRAGGGSLKSDHLSEENLQRVRALNDIA 390 G+G + ++ L +G L+ + + ED R + ++ + NLQ + ++A Sbjct: 210 LGIGFVAYSPLGRGFLTGAIQRFEDLAEDD--FRRSNPRFQGENFA-RNLQLAEKVKEMA 266 Query: 391 KARGQTLAQMALAWVL-RDPRVTTTLIGASSSKQIRENVAALDKLGFSADELAAID 445 G T +Q+ALAWV+ +DP + I + ++ E+ A + + ++L A+D Sbjct: 267 GQHGCTPSQLALAWVMAQDPHIVP--IPGTKRRRYLEDNAGSVGVKLAPEDLQALD 320 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 105 bits (263), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 108/343 (31%), Positives = 164/343 (47%), Gaps = 45/343 (13%) Query: 137 GRSGLKLPLLSLGMWHNFGDTTSLATQREMLRTAFDLGITHFDLANNYGPP-----YGSA 191 GR+ LK+ L++LG +G+ S A L A + GI D+A Y P G Sbjct: 7 GRTDLKVSLITLGTM-TWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAETQGLT 65 Query: 192 EANFGHLFKQDFLPYRDELIISTKA-GWDMWP-GPYGQGGG----SRKYVLASLDQSLKR 245 E G K+ RD+L+I+TK G P P GG RK + +L SL+R Sbjct: 66 ERYLGTWLKKSG--KRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGSLER 123 Query: 246 MNLDYVDIF----------------YSHRFDPET-PLEETMGALATAVQQGKALYVGVSS 288 + LD+VD++ Y H D T P+EET+ L+ V+ GK +G+S+ Sbjct: 124 LQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIGLSN 183 Query: 289 YSPEKTKEAAELLKQWKVPCLIH-QPSYNMFNRWIEQGLLDELERQGMGCITFTALAQGL 347 + + +Q + ++ Q YN+ NR E L + R+ +G + ++ LA G+ Sbjct: 184 ETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLAFGM 243 Query: 348 LSDKYLNGIPEDARINRAGGGSLK-SDHLSE-ENLQRVRALND---IAKARGQTLAQMAL 402 LS KYL+G R GG L + S N Q RA + +A+ G AQMAL Sbjct: 244 LSGKYLDGA-------RPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMAL 296 Query: 403 AWVLRDPRVTTTLIGASSSKQIRENVAALDKLGFSADELAAID 445 A+V P +T+ +IGA++ Q+R N+ + + L D LAAI+ Sbjct: 297 AYVNSRPFLTSNIIGATTLAQLRSNIDSAE-LQLGEDVLAAIE 338 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 78.2 bits (191), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 85/310 (27%), Positives = 140/310 (45%), Gaps = 33/310 (10%) Query: 137 GRSGLKLPLLSLGMWHNFGDTTSLATQ--REMLRTAFDLGITHFDLANNYGPPYGSAEAN 194 GR+G ++ + G W G ++ Q + L A D G++ D A+ YG G +E Sbjct: 7 GRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGD--GRSEQL 64 Query: 195 FGHLFKQDFLPYRDELIISTKAGWDMWPGPYGQGGGSRKYVLASLDQSLKRMNLDYVDIF 254 + K+ + ++TKAG + P G R + A +D+SLK + +D +D+ Sbjct: 65 IAQVLKER---GGERPFVATKAGRRLSPH-VASGYNDRAQLSAFIDRSLKNLGVDCLDLV 120 Query: 255 YSHRFDPETPL---EETMGALATAVQQGKALYVGVSSYSPEKTKEAAELLKQWKVPCLIH 311 H P T + +E G L V GK + GVS EK +EA + ++ V + Sbjct: 121 QLH--CPPTEVYYQQEVFGHLDALVAAGKIRHYGVSV---EKVEEALKAIEYPNVKSV-- 173 Query: 312 QPSYNMFNRWIEQGLLDELERQGMGCITFTALAQGLLSDKY---LNGIPEDAR-INRAGG 367 Q +N+F + L E +R+ + I LA GLL+ K +D R NR G Sbjct: 174 QIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGE 233 Query: 368 G-------SLKSDHLSEENLQRVRALNDIAKARGQTLAQMALAWVLRDPRVTTTLIGASS 420 S ++ ++++RAL +G +AQ AL W+L + ++T + GA + Sbjct: 234 AFDVGETFSGVPYEVALAAVEKIRAL----VPQGVPMAQFALRWILMESGISTVIPGARN 289 Query: 421 SKQIRENVAA 430 Q + N AA Sbjct: 290 VAQAQSNSAA 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,405,722 Number of extensions: 60050 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 6 Length of query: 463 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 374 Effective length of database: 1,198,455 Effective search space: 448222170 Effective search space used: 448222170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)