BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0234.0023.p (100 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 108 1e-25 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 108 bits (269), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 49/52 (94%), Positives = 51/52 (98%) Query: 26 RQRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDTTTQLLNTAGVF 77 R+RIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGD T+QLLNTAGVF Sbjct: 17 RKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQLLNTAGVF 68 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.131 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 232,033 Number of extensions: 7388 Number of successful extensions: 32 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 32 Number of HSP's successfully gapped: 1 Length of query: 100 Length of database: 1,619,870 Length adjustment: 69 Effective length of query: 31 Effective length of database: 1,293,155 Effective search space: 40087805 Effective search space used: 40087805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 123 (52.0 bits)