BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0233.0001.p (116 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313461|ref|YP_003777553.1| prolin-rich signal peptide prot... 117 2e-28 gi|300313462|ref|YP_003777554.1| hypothetical protein Hsero_4176... 80 5e-17 >gi|300313461|ref|YP_003777553.1| prolin-rich signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 255 Score = 117 bits (293), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 55/78 (70%), Positives = 63/78 (80%) Query: 26 FTSSGEIGAFGVEPELYTEKKRNRAATNTHFNRERNVVSFSASTTTYPRAGGEQDRASLI 85 F+S GE+ A+GV P LYTEKK+ R+ATNTHFNR + +SFSAS T YPR GGEQDR SLI Sbjct: 92 FSSEGELDAWGVSPTLYTEKKKFRSATNTHFNRNGDSISFSASETRYPRRGGEQDRGSLI 151 Query: 86 WQLAAIGRGDSTKFVPGA 103 WQLAAIGRGD +F PGA Sbjct: 152 WQLAAIGRGDPAQFQPGA 169 >gi|300313462|ref|YP_003777554.1| hypothetical protein Hsero_4176 [Herbaspirillum seropedicae SmR1] Length = 253 Score = 79.7 bits (195), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 34/77 (44%), Positives = 50/77 (64%) Query: 27 TSSGEIGAFGVEPELYTEKKRNRAATNTHFNRERNVVSFSASTTTYPRAGGEQDRASLIW 86 TS G + A+G+ P+ + EK+ R + T FNRE+N ++F+ S +YP GGEQDR S+ W Sbjct: 91 TSEGAVDAYGLAPDRFVEKRLRREPSTTTFNREQNKITFTQSAESYPLQGGEQDRTSITW 150 Query: 87 QLAAIGRGDSTKFVPGA 103 Q AI R ++ K PG+ Sbjct: 151 QFVAIARANAAKMKPGS 167 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.125 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 349,464 Number of extensions: 11647 Number of successful extensions: 31 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 31 Number of HSP's successfully gapped: 2 Length of query: 116 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 43 Effective length of database: 1,274,215 Effective search space: 54791245 Effective search space used: 54791245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 124 (52.4 bits)