BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0232.0002.p (455 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 226 4e-60 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 221 1e-58 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 213 2e-56 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 119 6e-28 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 108 1e-24 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 226 bits (575), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 150/460 (32%), Positives = 248/460 (53%), Gaps = 17/460 (3%) Query: 6 SLTVRLTALFALASTAVLVGLGLLIISTVAKHFTDQDEQLLEKELELIRMVVNERXAAIT 65 SL++RL ALF L + +L LG + ++ +D++ L + I +V E A++ Sbjct: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLV-EHAASLE 66 Query: 66 GTFG------EALHNHPGFFVHISSSNGQPLYSTLAGATEP--ILAAAASSRSFTVATGG 117 + L H V I+ ++G L + P I + R++ Sbjct: 67 DILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIRAWDSIQS- 125 Query: 118 HQKFQAIKARLKDEA----SGDNLDVIVAVDTDIHDHFMRNFQTTLILYVAGSALIVVLL 173 K ++AR SG+ V+V + +++ + AL+ +L+ Sbjct: 126 -WKLGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALLAILM 184 Query: 174 SWWAARRGLAPLRAMSDKVQVVSAHNFGERMPVETLPIEIADLAAKLNAMLERLQRDFNR 233 S++ R GL PLR M+ + + VSA+ R+ ++ P E+ ++ N ML+RL R F + Sbjct: 185 SYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRSFQQ 244 Query: 234 ITNFSSDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEELQRLARTISDMLFL 293 ++ FS+D+AH++RTP+ NL+ QT V L + R + Y+ LSSN EE +RL+R + MLFL Sbjct: 245 LSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFL 304 Query: 294 AQTENGISLPSHEPLSLQDEVAALFDFYDALAEEKGVRLLLTGEASIKGDRLMVRRALSN 353 A+ EN + + L + E+ + D+++ +A+E VRL++ G ++ D ++RRA+ N Sbjct: 305 ARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRRAVGN 364 Query: 354 LISNAMRYTPAACAILVSIEKSESDILVHVENDGPEIPAEYLPHLFDRFYRADKSRTKLD 413 L++NA+RYTPA I + ++ + + + V N G IPAE L LFDRFYRAD SR+ Sbjct: 365 LVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRSSA- 423 Query: 414 TDSAGLGLSITKAIMRAHQGEVAVQSGRGK-TRFTLRFSG 452 S+GLGL+I ++IM H G+ +V S G+ T F+LRF G Sbjct: 424 GGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFPG 463 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 221 bits (562), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 115/309 (37%), Positives = 181/309 (58%), Gaps = 3/309 (0%) Query: 137 LDVIVAVDTDIHDHFMRNFQTTLILYVAGSALIVVLLSWWAARRGLAPLRAMSDKVQVVS 196 +DV V VD + + R F ++ ++ V+ S+W AR GLAPLR MSD+ Q S Sbjct: 148 VDVTVGVDGEPYFEMRRTFLLATSVFSLLGIVLAVISSYWIARAGLAPLRQMSDRAQSFS 207 Query: 197 AHNFGERMPVETLPIEIADLAAKLNAMLERLQRDFNRITNFSSDIAHELRTPITNLLTQT 256 RMPVE +P E++ LA N L+R++ + ++ F++D+AHELRTP+ NL+ +T Sbjct: 208 PRKLSARMPVEAMPQELSVLALAFNGALDRIEDAYTQLEAFNADVAHELRTPLANLIGET 267 Query: 257 DVVLTQQRTNEAYRDTLSSNAEELQRLARTISDMLFLAQTENGISLPSHEPLSLQDEVAA 316 V L ++R+ ++D L SN EEL R+ ++DMLFLA+++ G + + P + DEV Sbjct: 268 QVALARERSAGEFKDVLQSNLEELDRIRSIVNDMLFLARSDQGEAATARVPARVADEVQK 327 Query: 317 LFDFYDALAEEKGVRLLLTG--EASIKGDRLMVRRALSNLISNAMRYTPAACAILVSIEK 374 DF++ + ++ G+R+ + G EA+ + + +RAL+NLI NA++++P + + I Sbjct: 328 TVDFFEFVLDDSGMRIEVAGDTEATAMMETALFKRALTNLIQNAIQHSPEGALLRIDIAC 387 Query: 375 SESDILVHVENDGPEIPAEYLPHLFDRFYRADKSRTKLD-TDSAGLGLSITKAIMRAHQG 433 E + V V N G I +LP LFDRFYR D +R ++ + GLGL+I KA+ R H G Sbjct: 388 QEETVRVAVSNPGKPIDEHHLPRLFDRFYRVDSARQDINGSHGHGLGLAIVKAVARMHGG 447 Query: 434 EVAVQSGRG 442 S G Sbjct: 448 RAFASSKEG 456 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 213 bits (543), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 151/469 (32%), Positives = 241/469 (51%), Gaps = 37/469 (7%) Query: 6 SLTVRLTALFALASTAVLVGLGLLIISTVAKHFTDQDEQLLEKELELIR----------- 54 +LT R+TALFAL ++ V+ GLG + + + + + +Q L +E R Sbjct: 12 TLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVEQ 71 Query: 55 ----------MVVNERXAAITGTFGEA--LHNHPGFFVHISSSNGQPLYSTLAGATEPIL 102 M+ NE+ I GEA + ++P V P T P+ Sbjct: 72 MEQRPALFESMMGNEQDVRIFRRQGEAPFIQSNPDHLV--------PPAMTPLPVGSPV- 122 Query: 103 AAAASSRSFTVATGGHQKFQAIKARLKDEASGDNLDVIVAVDTDIHDHFMRNFQTTLILY 162 ++AS R+ A G ++ + A GD ++++ A MR+++ + Sbjct: 123 -SSASLRTSQRADGVRVRW--VSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGA 179 Query: 163 VAGSALIVVLLSWWAARRGLAPLRAMSDKVQVVSAHNFGERMPVETLPIEIADLAAKLNA 222 A + ++ LL + RRGL PL AMS + ++ + R+ E +P E+ +A NA Sbjct: 180 AAAAVVLTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNA 239 Query: 223 MLERLQRDFNRITNFSSDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEELQR 282 ML+RL+ + + FS D+AHE+RTPI L+ Q+ V L Q+R+ E Y L SN EELQR Sbjct: 240 MLDRLEAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQR 299 Query: 283 LARTISDMLFLAQTENGISLPSHEPLSLQDEVAALFDFYDALAEEKGVRLLLTGEASIKG 342 L+R + ++LFLAQ ++ L L +E+ + D+++ +A+E+G+R + + Sbjct: 300 LSRIVENILFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQA 359 Query: 343 DRLMVRRALSNLISNAMRYTPAACAILVSIEKSESDILVHVENDGPEIPAEYLPHLFDRF 402 + +M RR +SNL+ NA+RY A I + E + VEN ++ A+ + +FDRF Sbjct: 360 NLIMWRRVVSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRF 419 Query: 403 YRADKSRTKLDTDSAGLGLSITKAIMRAHQGEVAVQS-GRGKTRFTLRF 450 YR D+SR+ T+S GLGL+I KAIM H G AV+ G RF+LRF Sbjct: 420 YRGDRSRSAF-TESNGLGLAIVKAIMALHGGTAAVECPAPGWIRFSLRF 467 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 119 bits (297), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 86/290 (29%), Positives = 149/290 (51%), Gaps = 15/290 (5%) Query: 166 SALIVVLLSWWAARRGLAPLRAMSDKVQVVSAHNFGERMPVETLPIEIADLAAKLNAMLE 225 +++ VVL+ A RRGL PL+++ ++ G + P+E+ L +N ++ Sbjct: 173 ASIFVVLV---AVRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNELMA 228 Query: 226 RLQRDFNRITNFSSDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEELQRLAR 285 R+ + NF +D+AH+LRTP+T L Q +++ + + +L+ ++R+ R Sbjct: 229 RINKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIR 288 Query: 286 TISDMLFLAQTENGISLPSH-EPLSLQDEVAALFDFYDALAEEKGVRLLLT-GEASIKGD 343 +L LA++E G+ E LSL V + A++K + L A ++GD Sbjct: 289 QSRQLLALARSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLRGD 348 Query: 344 RLMVRRALSNLISNAMRYTPAACAILVSIEKSESDILVHVENDGPEIPAEYLPHLFDRFY 403 R ++ + NLI NA+RY+P + V + + ++ VE+ GP I E+ +FDRFY Sbjct: 349 RFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFY 408 Query: 404 RADKSRTKLDTDSAGLGLSITKAIMRAHQGEVAV---QSGRGKTRFTLRF 450 RA+ +GLGL+I + I H G ++V + G+G T FT+RF Sbjct: 409 RANDK-----VAGSGLGLAIVREIAHDHGGVISVDCKEQGQG-TIFTVRF 452 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 87/274 (31%), Positives = 138/274 (50%), Gaps = 20/274 (7%) Query: 172 LLSWWAARRGLAPLRAMSDKVQVVSAHNFGERMPVET--LPIEIADLAAKLNAMLERLQR 229 +LSW A RGLAP+R + +V +A + P+ +P EI L LN +L RL+ Sbjct: 155 VLSWVAVSRGLAPVRKAAAEV---AARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKH 211 Query: 230 DFNRITNFSSDIAHELRTPITNLLTQTDVVLTQQRTNEAYRDTLSSNAEE-LQRLARTIS 288 F +D AHELRTP+T L QT L ++ +E R ++ + L+R + Sbjct: 212 ATETQRAFVADAAHELRTPLTALRLQTQ--LAERAVDEEERRAAFADLKNGLERTTHLVH 269 Query: 289 DMLFLAQTENGISLPSHEPLSLQDEVAALFDFYDALAEEKGVRLLLTGEAS------IKG 342 +L LA+ E S + L+ + ++ ++AE G+ L L+ + + G Sbjct: 270 QLLTLARQEPDAM--SFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTG 327 Query: 343 DRLMVRRALSNLISNAMRYTPAACAILVSIEKSESDIL-VHVENDGPEIPAEYLPHLFDR 401 + +R +NL+ NA+RYTPA + V ++ S + V V++ GP I LP +FDR Sbjct: 328 NADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDR 387 Query: 402 FYRADKSRTKLDTDSA---GLGLSITKAIMRAHQ 432 FYRA S+ + +A GLGL+I + I H+ Sbjct: 388 FYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLHR 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,262,173 Number of extensions: 50108 Number of successful extensions: 330 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 34 Length of query: 455 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 367 Effective length of database: 1,203,190 Effective search space: 441570730 Effective search space used: 441570730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)