BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0019.p (224 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 387 e-109 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 96 2e-21 gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 78 7e-16 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 76 2e-15 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 75 6e-15 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 387 bits (994), Expect = e-109, Method: Compositional matrix adjust. Identities = 192/250 (76%), Positives = 209/250 (83%), Gaps = 26/250 (10%) Query: 1 MIVLITGATAGFGA--------------------------LKEFGADKVLPLVLDVTDKN 34 M+VLITGATAGFGA +KE GADKVLPLVLDVT K+ Sbjct: 1 MVVLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGADKVLPLVLDVTSKS 60 Query: 35 SIKSALASLPQGWQDIDVLINNAGLALGLEPAHQVALEDWETMIDTNVKGLVTVTHHILP 94 SI++ LASL W+++DVLINNAGLALGL PAH+VAL DWETMIDTNVKGLVT+TH +LP Sbjct: 61 SIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLP 120 Query: 95 NMVKRGTGTIINLGSIAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGL 154 +MVKRG GTIINLGS+AGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGL Sbjct: 121 DMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAPGL 180 Query: 155 CGGTEFSNVRFKGNDSAAAKVYEGTTPLTAVDIAEAAFWVATLPAHVNINYIEMMPTCQG 214 CGGTEFSNVRFKG+D AAAKVYEGTTPLTA DIAEAAFWVAT+PAHVNINY+E+MPTCQG Sbjct: 181 CGGTEFSNVRFKGDDGAAAKVYEGTTPLTADDIAEAAFWVATMPAHVNINYMELMPTCQG 240 Query: 215 FGPLNIKRTQ 224 F P NIKR Q Sbjct: 241 FSPFNIKRKQ 250 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 96.3 bits (238), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 76/243 (31%), Positives = 117/243 (48%), Gaps = 32/243 (13%) Query: 2 IVLITGATAGFGA-----LKEFGADKVL---------PLVLDVT--------------DK 33 +V+ITGA++G G L GA VL LV D+T + Sbjct: 9 VVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVTTDVARR 68 Query: 34 NSIKSALASLPQGWQDIDVLINNAGLALGLEPAHQVALEDWETMIDTNVKGLVTVTHHIL 93 + +++ +A Q + IDVL+NNAG+ + L P ++ +E+W+ MID NVKG++ L Sbjct: 69 DDVEALVAKGEQHFGRIDVLVNNAGI-MPLAPMAKLKVEEWDRMIDVNVKGVLYGVAAAL 127 Query: 94 PNMVKRGTGTIINLGSIAGAYPYPG-GNVYGATKAFVDQFTLNLRADLVGTGVRATNLAP 152 P + +G IIN+ S+AG + G G VY ATK V T LR + GVR T ++P Sbjct: 128 PRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTE-APDGVRTTIISP 186 Query: 153 GLCGGTEFSNVRFKGNDSAAAKVYEGTTPLTAVDIAEAAFWVATLPAHVNINYIEMMPTC 212 G ++ K A YE + + +A A + PA+V+I+ + + P Sbjct: 187 GAVDSELKTHSSDKETSEALLAWYEANQ-IPSDSVARAIAYAIEQPANVDISEVVLRPVA 245 Query: 213 QGF 215 Q F Sbjct: 246 QEF 248 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 77.8 bits (190), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 66/193 (34%), Positives = 97/193 (50%), Gaps = 27/193 (13%) Query: 22 KVLPLVLDVTDKNSIKSALASLPQGWQDIDVLINNAGL----ALGLEPAHQVALEDWETM 77 ++LPL DV D +SI++A+ + +DVL+NNAG+ AL +P QV + Sbjct: 47 RILPL--DVADAHSIQAAV----EAAGPVDVLVNNAGIGNLGALEAQPMAQV-----REL 95 Query: 78 IDTNVKGLVTVTHHILPNMVKRGTGTIINLGSIAGAYPYPGGNVYGATKAFVDQFTLNLR 137 +TN G + +T +P M RG G +IN+ S P VY A+KA ++ FT +L Sbjct: 96 FETNTLGSIAMTQAFIPQMRARGQGLVINVTSSVTLRALPLLAVYTASKAAINAFTESLA 155 Query: 138 ADLVGTGVRATNLAPGLCGGTEFS-NVRFKGNDSA-------AAKVYE---GTTPLT-AV 185 +L GV+ + PG T F+ N R + S AA+V+E T LT A Sbjct: 156 LELAPLGVQVRLVLPGRSPQTRFAENARARMAASVPEPYAALAAQVFENFQAATQLTYAE 215 Query: 186 DIAEAAFWVATLP 198 D+AEA + A P Sbjct: 216 DVAEAVWQAANDP 228 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 75.9 bits (185), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 45/135 (33%), Positives = 72/135 (53%), Gaps = 1/135 (0%) Query: 21 DKVLPLVLDVTDKNSIKSALASLPQGWQDIDVLINNAGLALGLEPAHQVALEDWETMIDT 80 K + + DV D ++I +A+A IDVLINNAG + P V E ++ +I+ Sbjct: 56 SKSITIGADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIINI 115 Query: 81 NVKGLVTVTHHILPNMVKRGTGTIINLGSIAGAY-PYPGGNVYGATKAFVDQFTLNLRAD 139 N + +V T ++P M +G G IIN+ S+A + PG +Y A+K FV T + + Sbjct: 116 NARSVVAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKE 175 Query: 140 LVGTGVRATNLAPGL 154 L+ +R +APG+ Sbjct: 176 LLADKIRVNAVAPGV 190 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 74.7 bits (182), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 41/128 (32%), Positives = 69/128 (53%), Gaps = 1/128 (0%) Query: 29 DVTDKNSIKSALASLPQGWQDIDVLINNAGLALGLEPAHQVALEDWETMIDTNVKGLVTV 88 +V++ S K+A + ++DVL+NNAG+ + +++ +DW+ +IDTN+ L V Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQ-FRKMSKDDWDAVIDTNLNSLFNV 119 Query: 89 THHILPNMVKRGTGTIINLGSIAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRAT 148 T ++ M +RG G IIN+ S+ G G Y KA + FT+ L ++ GV Sbjct: 120 TKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVN 179 Query: 149 NLAPGLCG 156 ++PG G Sbjct: 180 TVSPGYIG 187 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 758,805 Number of extensions: 33730 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 29 Length of query: 224 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 142 Effective length of database: 1,231,600 Effective search space: 174887200 Effective search space used: 174887200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)