BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0016.p (245 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel f... 317 4e-88 gi|300309940|ref|YP_003774032.1| biopolymer transport transmembr... 88 6e-19 gi|300310025|ref|YP_003774117.1| biopolymer transport transmembr... 86 3e-18 gi|300312127|ref|YP_003776219.1| biopolymer transport transmembr... 84 7e-18 gi|300310500|ref|YP_003774592.1| biopolymer transport ExbB-like ... 58 7e-10 >gi|300310552|ref|YP_003774644.1| MotA/TolQ/ExbB proton channel family protein [Herbaspirillum seropedicae SmR1] Length = 234 Score = 317 bits (813), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 177/228 (77%), Positives = 191/228 (83%), Gaps = 18/228 (7%) Query: 1 MNVTQDLSFFALITNASVIVQFVMLLLLLASVFSWTYIFRKMFTIRSAR--SRXFRKMF- 57 MNVTQDLSF +LI NASV+VQ VMLLLL AS+ SWTYIFRKMFTIR+AR + F ++F Sbjct: 1 MNVTQDLSFISLIANASVLVQLVMLLLLSASIISWTYIFRKMFTIRAARVQTEEFERVFW 60 Query: 58 --------------TIRSARGHGGALERIFQAGMGEFNKAKAQVG-KGAAIDSTGVLDGA 102 R A G GGALERIFQAGMGEF KAKA V +G A+D + +LDGA Sbjct: 61 SGGNLSALYQDALSNRRKAGGAGGALERIFQAGMGEFTKAKASVAARGGAVDPSLLLDGA 120 Query: 103 RRAMRAAYQREMDALESHLAFLATVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA 162 RRAMRAAYQREMDALESHLAFLA+VGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA Sbjct: 121 RRAMRAAYQREMDALESHLAFLASVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVA 180 Query: 163 PGIAEALIATAIGLFAAIPAVIAYNRFSHDIDRLAIRFESFIEEFSNI 210 PGIAEALIATAIGLFAAIPAV+AYNR+SHDIDRLAIRFESFIEEFSNI Sbjct: 181 PGIAEALIATAIGLFAAIPAVVAYNRYSHDIDRLAIRFESFIEEFSNI 228 >gi|300309940|ref|YP_003774032.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 231 Score = 88.2 bits (217), Expect = 6e-19, Method: Compositional matrix adjust. Identities = 67/208 (32%), Positives = 97/208 (46%), Gaps = 18/208 (8%) Query: 5 QDLSFFALITNASVIVQFVMLLLLLASVFSWTYIFRKMFTIRSAR--SRXFRKMFTIRSA 62 Q++ F + V LLL+ S+ SW YI K + R S + + + Sbjct: 3 QNIGFAHYWAQGDAVSHSVAYLLLIMSIASWYYILSKAWAAWRIRRGSNALERFWQAPTL 62 Query: 63 RGHGGALERI----FQAGMGEFNKAKAQVGK--------GAAIDSTGVLDGARRAMRAAY 110 A++R+ M E A +G AA D ++ R +R Sbjct: 63 ADATAAMQRLDSEQIYTPMAERAAEAADIGTRQQGTPSLNAATDPGELIT---RTLRREI 119 Query: 111 QREMDALESHLAFLATVGSVSPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALI 170 R LE L LA+VGS +P+VGLFGTVWGI +A ++ + VA + EALI Sbjct: 120 NRVSARLERGLTLLASVGSTAPFVGLFGTVWGIYHALVAVSASGTVQIDKVAGPVGEALI 179 Query: 171 ATAIGLFAAIPAVIAYNRFSHDIDRLAI 198 TA+GL AIPAV+AYN F+ I+R+ + Sbjct: 180 MTALGLLVAIPAVLAYNAFTR-INRVVL 206 >gi|300310025|ref|YP_003774117.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 85.9 bits (211), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 59/178 (33%), Positives = 87/178 (48%), Gaps = 11/178 (6%) Query: 19 IVQFVMLLLLLASVFSWTYIFRK---MFTIRSARSRXFRKMFTIRS------ARGHGGAL 69 + + V +LLL S+ SW I K + +R A + + + + G Sbjct: 18 VTKGVAVLLLAMSIASWYVIITKALQLLRLRKASAAAGHQFWDTTNLPEGVATLGQDNPF 77 Query: 70 ERIFQAGMGEFNKAKAQVGKGAAIDSTGVLDGARRAMRAAYQREMDALESHLAFLATVGS 129 + +AG+ + A KG D V D ++R A L++ +A LA+VGS Sbjct: 78 AEVAKAGVTAMSHHTAH--KGHLHDQLSVSDWVTLSLRQAIDEASGKLQAGMAVLASVGS 135 Query: 130 VSPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALIATAIGLFAAIPAVIAYN 187 +P+VGLFGTVWGI +A + QA++ VA + EALI TA+GL AIPA YN Sbjct: 136 TAPFVGLFGTVWGIYHALVSIGTTGQASIDKVAGPVGEALIMTALGLAVAIPATFGYN 193 >gi|300312127|ref|YP_003776219.1| biopolymer transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 84.3 bits (207), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 62/197 (31%), Positives = 96/197 (48%), Gaps = 21/197 (10%) Query: 18 VIVQFVMLLLLLASVFSW-TYIFRKMFTIRSARS--RXFRKMFTIRSARGH----GGALE 70 V+ +F++ +LL S SW I + M R +R + ++ + S G G + Sbjct: 15 VVTRFLITILLGMSSVSWIVMIVKGMANRRLSRIGLKAHKEFWAAHSIEGGIAAIGAGTD 74 Query: 71 RIFQAGMGEFNKAKAQVGKGAAIDSTGVLDGARRAMRAAYQREMDALESHLAFLATVGSV 130 + +A+AQ + ST + +R ++ L+ LA LA++GS Sbjct: 75 NPYSNIATAAVEAQAQKDQPLLEGSTSNTEWVAYCVRNELDEQIAHLQGGLAVLASIGST 134 Query: 131 SPYVGLFGTVWGIMNAFRGLANVQQATLAAVAPGIAEALIATAIGLFAAIPAVIAYN--- 187 SP++GLFGTVWGI +A + QA+L VA + E+L+ TA+GLF AIPAV+ YN Sbjct: 135 SPFIGLFGTVWGIYHALIAIGASGQASLDHVAGPVGESLVMTAVGLFVAIPAVLGYNYVA 194 Query: 188 -----------RFSHDI 193 RFSH + Sbjct: 195 RGNRTVAQKLMRFSHQL 211 >gi|300310500|ref|YP_003774592.1| biopolymer transport ExbB-like transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 201 Score = 58.2 bits (139), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 54/198 (27%), Positives = 87/198 (43%), Gaps = 19/198 (9%) Query: 27 LLLASVFSWTYIFRKMFTIRSARSRXFRKMFTIRSARGHGGALERIFQAG--MGEFNKAK 84 LL+AS+ + ++ +R RSR + R++Q+G + Sbjct: 15 LLIASIIGLALVIERLVYLR--RSRILPPDLL--------NQVVRVYQSGRITDDVADKL 64 Query: 85 AQVGKGAAIDSTGV--LDGARRAMRAAYQREMDA----LESHLAFLATVGSVSPYVGLFG 138 Q + + G+ +D R M+ A + A LE L L T+ S++P +GLFG Sbjct: 65 EQNSPLGHVLAAGLRNVDAPREVMKEAIEEAGQAAAHRLERFLTTLGTIASLAPLMGLFG 124 Query: 139 TVWGIMNAFRGLANVQQATLAAVAPGIAEALIATAIGLFAAIPAVIAYNRFSHDIDRLAI 198 TV G++ F N A +A GI+ AL T GL A+PA++ Y F +D + Sbjct: 125 TVVGMIEIFAS-QNAAGTNPAQLAHGISIALYNTGFGLAIAMPALVFYRHFRALVDGFVV 183 Query: 199 RFESFIEEFSNIXADRRQ 216 E +F +I D R+ Sbjct: 184 DMEQQAVKFVDIVHDVRR 201 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 640,490 Number of extensions: 25708 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 148 Number of HSP's successfully gapped: 5 Length of query: 245 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 162 Effective length of database: 1,226,865 Effective search space: 198752130 Effective search space used: 198752130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)