BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0230.0010.p (425 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 94 2e-20 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 90 3e-19 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 75 1e-14 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 60 5e-10 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 57 3e-09 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 94.4 bits (233), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 95/350 (27%), Positives = 160/350 (45%), Gaps = 39/350 (11%) Query: 12 AGLAVSFSIAAFSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVPN 71 A + ++F+ AA+S Q+ +KI + PLSG A G + ++ +A D +N AKG Sbjct: 12 AAIGLAFAGAAYS----QEVVKIAHVGPLSGPNAHMGKDNENGARMAVDELN--AKG--- 62 Query: 72 LPFSAGGGLPNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATTS 131 F+ GG K K +LV D +P+ A +L+ Q KV AV+G S T S Sbjct: 63 --FTIGG-----KKVKFELVGEDDASDPKQATAVATKLVDQ-KVAAVIGHLNSGTTIPAS 114 Query: 132 QVAERYKVPYLNPESSSASLTQRGFKWFFRTTAHDDLFVTNFYEFFKELEAKKGIKISKL 191 ++ +P ++P +++ TQ+GFK FR A+D ++ + K I + Sbjct: 115 KIYSDAGIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAV--- 171 Query: 192 GAFNENTLWGNETTKLETKLSKDRGYDLVKTIVYPAKSTQLTSEVQLLKAAAPQVVMQSS 251 ++ T +G K +K G +V T K+T + + LK P VV Sbjct: 172 --IDDRTAYGQGVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGG 229 Query: 252 YLGDAILS--MKTYKELGFTPDMILANDAGFIDTEFVKTLGKDA---DFIVTRDVWSLDL 306 DA+ ++ K+LG + + + I T + L D D ++ + +D Sbjct: 230 M--DAVGGPMLRQMKQLGISAKFMGGDG---ICTGSLPGLAGDGLGDDQVICAEAGGVDE 284 Query: 307 AGKNPLIKQVNDL---FNARFKINFTGNSSRTFTGLMTLADAINRAGSTD 353 AGK K ++D + +F I+ N++ + MT+ADA+ +AGS D Sbjct: 285 AGK----KGMDDFRAAYKKKFGIDVVYNAAYAYDATMTVADAMAKAGSAD 330 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 101/371 (27%), Positives = 162/371 (43%), Gaps = 34/371 (9%) Query: 5 VFKRVFKAGL---AVSFS-IAAFSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAAD 60 +FKRV +GL A S + I AF+ + Q +KIG PL+G AS G + + L +A + Sbjct: 1 MFKRV--SGLNIIAASLALIPAFAMAQETQVVKIGFSSPLTGPQASAGKDNQGGLMMAIE 58 Query: 61 IINNGAKGVPNLPFSAGGGLPNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVG 120 +N P + GG K K ++ D Q +P+ G A++L Q V A+VG Sbjct: 59 RLNA-------QPITVGG-----KKIKFDVIAEDDQADPKSGVAVAQKLADQ-GVKAIVG 105 Query: 121 AYFSNVTATTSQVAERYKVPYLNPESSSASLTQRGFKWFFRTTAHDDLFVTNFYEFFKEL 180 Y S VT S+V + + +S+ +TQ+GF FR A D L Sbjct: 106 PYNSGVTIPASRVYNDAGI-VVATVASNPKITQQGFATLFRVAASDSQLGGKM-----AL 159 Query: 181 EAKKGIKISKLGAFNENTLWGNETTKLETKLSKDRGYDLVKTIVYPAKSTQLTSEVQLLK 240 A K +K ++ ++ T +G + K++K G D+V T K+T T+ + +K Sbjct: 160 YAAKELKFKRVAVIDDRTAYGQGLAQEFIKVAKANGIDVVSTDFTNDKATDFTAILTSIK 219 Query: 241 AAAPQVVMQSSYLGDAILSMKTYKELGFTPDMILANDAGFIDTEFVKTLGKDA---DFIV 297 P V Y + K+LG D+ L G E + LG DA Sbjct: 220 GKKPDAVFLGGYAPQGGPIKRQMKQLGV--DVPLMGGDGICSPEMGR-LGGDAIGESVYC 276 Query: 298 TRDVWSLDLAGKNPLIKQVNDLFNARFKINFTGNSSRTFTGLMTLADAINRAGSTDAEAI 357 T+ LD A + K +D + ++ + + G+M +A A+ +A S D + Sbjct: 277 TQGGTMLDKAKEG---KVFSDEYQKKYNRPAETYAVSFYDGMMLIAQAMKQANSVDPKQF 333 Query: 358 RKALSETNIPG 368 AL++ + G Sbjct: 334 GPALAKISYKG 344 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 97/406 (23%), Positives = 179/406 (44%), Gaps = 49/406 (12%) Query: 11 KAGLAVSFSIAAFSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVP 70 +A +A + A ++QA IKIGV P +G +A G M+D ++LA IN Sbjct: 6 QAFVAAALMSLALTSQAAD-PIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEIN------- 57 Query: 71 NLPFSAGGGLPNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATT 130 +AGG L G +I+L+ D + + G A+ LI +EKVVA VG + V + Sbjct: 58 ----AAGG----LLGRQIQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALAS 109 Query: 131 SQVAERYKVPYLNPESSSASLTQRGFK----WFFRTTAHDDLFVTNFYEFFKELEAKKGI 186 + + K+P +N ++ +T++ K + FRT+A D + + EA Sbjct: 110 QRFYQEAKIPVINNVATGTVITKQFEKEKDNYVFRTSAADKIQAKMIAD-----EAVDKQ 164 Query: 187 KISKLGAFNENTLWGNETTKLETKLSKDRGYDLVKTIVYPAKSTQLTSEVQLLKAAAPQV 246 K +K+ ++T +G + K+ D+ V Y K +T+++ K Q Sbjct: 165 KFTKVAILADSTNYGQLGREDLEKVLADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQA 224 Query: 247 VMQSSYLGDAILSMKTYKE--------LGFTPDMILANDAGFIDTEFVKTLGKDADFIVT 298 V+ + +G + + E +G P + + N FID GK+ + Sbjct: 225 VL-TYGIGPELAQIANGMEKLNWHVPIIGSWP-LSMGN---FIDNS-----GKNGNGARM 274 Query: 299 RDVWSLDLAGKNPLIKQVNDLFNARFKINFTG---NSSRTFTGLMTLADAINRAGSTDAE 355 + D G P K + + +K++ ++++ + ++ LA AI +AG+TD + Sbjct: 275 PQTFIQD--GNTPKRKAFIEAYQKAYKVDRMPSAVSAAQGYDSILLLAAAIKQAGTTDGD 332 Query: 356 AIRKALSETNIPGNKLIMPW-KGIKFDEHGQNTLSSGILLQIINGK 400 +R AL N ++ + K D+H + ++ ++ NG+ Sbjct: 333 KVRAALENLNTKVEGVVTTYDKPFTHDDHEAIKPAMVVMGEVKNGR 378 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 59.7 bits (143), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 84/366 (22%), Positives = 141/366 (38%), Gaps = 55/366 (15%) Query: 21 AAFSAQAQQQEIKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVPNLPFSAGGGL 80 A S A +IK+GV L+G AA G +K+ LA++ IN AKG Sbjct: 17 ATLSQAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEIN--AKG------------ 62 Query: 81 PNLKGAKIKLVFADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATTSQVAERYKVP 140 + G K+ LV D QG + ++LI Q+KV+AV G SN +A KV Sbjct: 63 -GVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVV 121 Query: 141 YLNPESSSASLTQRGFKWFFRTTAHDDLFVTNFYEFFKELEAKKGIKISKLGAFNENTLW 200 +++ +T G F + D+ K KK ++ Sbjct: 122 VFGTSNTADGITAMGPFTFRNSVMEADVLPVTVKAAVKHFGIKK-----------VAVIY 170 Query: 201 GNETTKLETKLSKDRGYDLVK------------TIVYPAKSTQLTSEVQLLKAAAPQVVM 248 GN+ ++ GYD+ K T Y +++ +KA P ++ Sbjct: 171 GNDDAFTKS------GYDVFKATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIV 224 Query: 249 QSSYLGDAILSMKTYKELGFTPDMILANDAGFIDTEFVKTLGKDA-DFIVTRDVWSLDLA 307 S +A + + LG I N +++ + + KDA D + WS + Sbjct: 225 CSCLAEEAANIILQTRALGMKQPFIGGNG---LNSPKLFEIAKDAGDNTLMGSPWSAE-- 279 Query: 308 GKNPLIKQVNDLFNARFKINFTGNSSRTFTGLMTLADAINRAG-----STDAEAIRKALS 362 + P K + A+F + +++ + + +ADA+ + D +A+R AL Sbjct: 280 NQTPANKAFITAYKAKFGADPDQFAAQAYDAMYIVADALKNVKLSGNLAKDRDALRAALP 339 Query: 363 ETNIPG 368 I G Sbjct: 340 AVKIDG 345 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 57.0 bits (136), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 80/389 (20%), Positives = 153/389 (39%), Gaps = 43/389 (11%) Query: 32 IKIGVIYPLSGAAASTGAEMKDALELAADIINNGAKGVPNLPFSAGGGLPNLKGAKIKLV 91 + IG PLSG + G M++ ++LA N +G+ GG K +++L+ Sbjct: 40 LAIGFAGPLSGPSGGVGKSMQNGVQLALAEANR--RGL-----RIGG-----KSWQLRLI 87 Query: 92 FADSQGNPQVGATEAERLITQEKVVAVVGAYFSNVTATTSQVAERYKVPYLNPESSSASL 151 D + +P A L+ Q++V+AVVG + S + + V R V + P + S L Sbjct: 88 AQDDRADPATAEFVARSLV-QQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRL 146 Query: 152 TQRGFKWFFRTTAHDDLF--VTNFYEFFKELEAKKGIKISKLGAFNENTLWGNETTKLET 209 T + FRT +++ + Y K LE + + I ++ T +G + Sbjct: 147 TAMSYPRIFRTIPNNESLGRLAAGYAVDK-LETRTIVTI------DDRTPFGQGLAEQFA 199 Query: 210 KLSKDRGYDLVKTIVYPAKSTQLTSEVQLLKAAAPQVVM------QSSYLGDAILSMKTY 263 + + +RG +V K++ + + + P ++ Q+ L +I +K + Sbjct: 200 RTASERGAQVVGRYSVSDKTSDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLH 259 Query: 264 KELGFTPDMILANDAGFIDTEFVKTLGKDADFIVTRDVWSLDLAGKNPLIKQVNDLFNAR 323 L +P G + F+ G DA+ + + K P K ++ Sbjct: 260 ARLMASP--------GTLGLPFLMRAGPDANGALVLEPGPPQ--DKMPGWKSFRQRYSEN 309 Query: 324 FKINFTGNSSRTFTGLMTLADAINRAGSTDAEAIRKALSETNIPGNKLIMPWKGIKFDEH 383 F + + + + I +AGS D E I +A+ G + F+E Sbjct: 310 FDSDMDLYAVFAYEAAQAIIQGIRQAGSADPERIAEAMHRLRFEGVS-----GPVAFNEE 364 Query: 384 GQNTLSSGILLQIINGKYYTVWPFDMATR 412 G S + ++ + ++ V F+ R Sbjct: 365 GDLLQPSFTMYEVKDQRWQPVARFNGLPR 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,202,734 Number of extensions: 47528 Number of successful extensions: 167 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 9 Length of query: 425 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 337 Effective length of database: 1,203,190 Effective search space: 405475030 Effective search space used: 405475030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)