BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0230.0006.p (344 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 120 2e-28 gi|300313300|ref|YP_003777392.1| branched-chain amino acid ABC t... 104 1e-23 gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 100 2e-22 gi|300312238|ref|YP_003776330.1| amino acid ABC transporter perm... 99 7e-22 gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC t... 97 1e-21 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 86/256 (33%), Positives = 136/256 (53%), Gaps = 12/256 (4%) Query: 1 VLALA-LPLYVTSPSHQNLAILILMAAQIGVSWNIVGGYAGQVSLGHVAFYGIGAYTSAI 59 ++ALA +P+ V +P + +L IL+ A + +IV GY GQVSLGH +GIG+Y + + Sbjct: 13 IVALAGIPMVVHNPYYLHLIETILIYAILLFGLDIVVGYTGQVSLGHAGLFGIGSYAAGV 72 Query: 60 LFSVYGVNPWFAMIVGGV-IAALVSLVIGWSCFRLKGHYFAMATIAVAEIIQIVFTNWDF 118 LF G+ P F + + + A+ ++ R+ G Y AM T+A IIQI+ F Sbjct: 73 LFFKLGM-PIFVTVPAAIAVTAVFGAILALPALRVTGPYLAMVTLAFGTIIQILINEMTF 131 Query: 119 AGAAVGLTIPMGEGGWANMIFAGKEP---YYYVALGLLLLTLLANFIIERSFLGYYFRAI 175 P+G MI K ++Y+ L++L++L I +S LG F+A+ Sbjct: 132 LTEG-----PLGIKLQKPMIGGDKMSEVQFFYLVAVLMVLSMLVVHRILKSNLGRAFQAL 186 Query: 176 KDEPDAARSLGVSLSKYKQIAFAVSSFFTAMGGSLYAQKELYIDPASVLGTGMSIKMALV 235 +D P A+ +GVS+ +YK AF +S+ + G+LYA E YI P + ++I L Sbjct: 187 RDSPIASDCMGVSVYRYKVYAFIISAALAGLSGALYAYSEEYISP-NTYNFELTIMFLLA 245 Query: 236 AILGGIGTLFGPLVGA 251 I+GG + G L+GA Sbjct: 246 VIMGGRKSRIGSLIGA 261 Score = 78.6 bits (192), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 41/88 (46%), Positives = 61/88 (69%) Query: 256 RALAVKPKLLLMDEVMAGLNPTEVRETVDVVLKIRDQGISCLIVEHVLEGIMPIANQIVV 315 RAL + P LLL+DE AGL +++E V ++ KIR+ GI+ +++EH ++ +M I + + V Sbjct: 553 RALGLNPSLLLLDEPAAGLTAPDIKELVAIIRKIREAGITIILIEHHMDVVMSICDTVTV 612 Query: 316 LDYGKKIADGSPHDVSNDPHVIAAYLGD 343 LD+G+KIA+G P V DP VI AYLG Sbjct: 613 LDFGQKIAEGKPAQVQADPKVIEAYLGS 640 >gi|300313300|ref|YP_003777392.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 597 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 69/223 (30%), Positives = 115/223 (51%), Gaps = 4/223 (1%) Query: 34 IVGGYAGQVSLGHVAFYGIGAYTSAILFSVYGVNPWFAMIVGGVIAALVSLVIGWSCFRL 93 ++ G G S G AF G+GAYT++ L +V+GV+PW + VG I + + V+G R+ Sbjct: 47 LLTGIGGMTSFGQAAFVGMGAYTTSYLTTVHGVSPWLTLPVGLFITGVSAYVLGRITLRM 106 Query: 94 KGHYFAMATIAVAEIIQIVFTNWDFAGAAVGLT-IPMGEGGWANMIFAGKEPYYYVALGL 152 GHY +ATIA + +F DF G G++ IP + A + A + YY+ + Sbjct: 107 SGHYLPLATIAWGISLYFLFGKVDFLGKYDGISGIPALQ--LAGLDLAKERHLYYLIWAI 164 Query: 153 LLLTLLANFIIERSFLGYYFRAIKDEPDAARSLGVSLSKYKQIAFAVSSFFTAMGGSLYA 212 +L + + S G RA+K A ++GV +YK + F ++ ++ G L+A Sbjct: 165 VLAAAWVSLNLLDSRAGRAIRALKGGRSMAEAMGVDTGRYKILIFVFAALLASVSGWLFA 224 Query: 213 QKELYIDPASVLGTGMSIKMALVAILGGIGTLFGPLVGAGXKK 255 + ++P S M I+ + ++GGI ++G L+GAG K Sbjct: 225 HMQRTVNP-SPFSLAMGIEYLFIVVVGGIAHIWGALLGAGVLK 266 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 31/74 (41%), Positives = 49/74 (66%) Query: 269 EVMAGLNPTEVRETVDVVLKIRDQGISCLIVEHVLEGIMPIANQIVVLDYGKKIADGSPH 328 E AGL E + V+ ++R G+S L+VEH +E +M + + IVV+++G KIA+G+P Sbjct: 521 EPAAGLRHLEKQALSKVLQELRADGMSILLVEHDMEFVMELTDHIVVMEFGAKIAEGTPQ 580 Query: 329 DVSNDPHVIAAYLG 342 +++ P VI AYLG Sbjct: 581 EIARHPAVIEAYLG 594 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 79/243 (32%), Positives = 119/243 (48%), Gaps = 13/243 (5%) Query: 13 PSHQNLAILILMAAQIGVSWNIVGGYAGQVSLGHVAFYGIGAYTSAILFSVYGVNPWFAM 72 P L + IL+A S + + G G VS GH A++G+GAY +A++ AM Sbjct: 345 PYLPVLMVEILVAVLFAASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPLAM 404 Query: 73 IVGGVIAALVSLVIGWSCFRLKGHYFAMATIAVAEIIQIVFTNW-DFAGAAVGLTIPMGE 131 + G A L +L+ GW C RL G Y AM T+A A+I+ W D G + GL Sbjct: 405 LAGAGTAMLAALLFGWFCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLV----- 459 Query: 132 GGWANMIFAGKEPYYYVALGLLLLTLLANFIIERSF---LGYYFRAIKDEPDAARSLGVS 188 G A YYY+A LL + L +++ R G RA +D A++LG+ Sbjct: 460 GLRTAAALASPLAYYYLA---LLCSALGVYLLRRVIHAPFGLALRAARDASGRAQALGMD 516 Query: 189 LSKYKQIAFAVSSFFTAMGGSLYAQKELYIDPASVLGTGMSIKMALVAILGGIGTLFGPL 248 + + FA++ F + G L+A + I P V G S+ ++ +LGG+ +L GPL Sbjct: 517 VRALQWGGFALAGLFCGLAGVLFAFSKGSISP-EVAGVNRSVDGLVMVLLGGVQSLAGPL 575 Query: 249 VGA 251 +GA Sbjct: 576 LGA 578 >gi|300312238|ref|YP_003776330.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 98.6 bits (244), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 71/214 (33%), Positives = 111/214 (51%), Gaps = 15/214 (7%) Query: 39 AGQVSLGHVAFYGIGAYTSAILFSVYGVNPWFAMIVGGVIAALVSLVIGWSCFRLKGHYF 98 G +SL + AF GIGAYT+++L + + A+ GGV+ ALV+LVIG RL G Y Sbjct: 34 CGLLSLANAAFMGIGAYTASLLTMQFEMPFPIALAAGGVLPALVALVIGIPTLRLSGVYL 93 Query: 99 AMATIAVAEIIQIVFTNWDFAGAAVGLT-IPMGEGGWANMIFAGKEPYYYVALGLLLLTL 157 AMAT+ E+++++ N D G +GL +P W ++ G Y L Sbjct: 94 AMATLGFGEVVRVIVLNLDITGGPMGLNGVPPITEWWHIVLLLGVTIYVLARL------- 146 Query: 158 LANFIIERSFLGYYFRAIKDEPDAARSLGVSLSKYKQIAFAVSSFFTAMGGSLYAQKELY 217 RS +G F AIK++ AAR +GV+++ YK +AF + + + G L A Sbjct: 147 ------RRSKIGRAFEAIKEDEIAARLMGVNVAGYKLLAFVIGAAIAGVAGGLNAHFTFT 200 Query: 218 IDPASVLGTGMSIKMALVAILGGIGTLFGPLVGA 251 I P + G ++ + +A+ GG L GP++G+ Sbjct: 201 IGPNNY-GFENAVDILTMAVFGGTSNLIGPMIGS 233 >gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 408 Score = 97.4 bits (241), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 76/272 (27%), Positives = 137/272 (50%), Gaps = 25/272 (9%) Query: 3 ALALPLYVTSPSHQNLAILILMAAQIGVSWNIVGGYAGQVSLGHVAFYGIGAYTSAILFS 62 AL P + S + ++ L L+ +G+ NIV G+AG + LG+V FY +GAYT A+ Sbjct: 91 ALVWPFF-GSRGYVDVMTLALIYVVLGLGLNIVVGFAGLLDLGYVGFYAVGAYTYALCNQ 149 Query: 63 VYGVNPWFAMIVGGVIAALVSLVIGWSCFRLKGHYFAMATIAVAEIIQIVFTNW-DFAGA 121 +G+ W + + +AL ++G+ RL+G Y A+ T+ EII+++ N G Sbjct: 150 YFGLTFWECVPLAAAASALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRLLLNNMTSITGG 209 Query: 122 AVGLT--------------IPMGEGGWA-----NMIFAGKEP---YYYVALGLLLLTLLA 159 G++ P+ EGG + + G Y++AL ++L T Sbjct: 210 PDGVSGIPKPTVFGLVMARNPVTEGGTTFHQLFGLSYQGGHMVIFLYFLALLMVLFTYFV 269 Query: 160 NFIIERSFLGYYFRAIKDEPDAARSLGVSLSKYKQIAFAVSSFFTAMGGSLYAQKELYID 219 + R +G + A++++ A RSLG++ +K K AF + + F + GS +A ++ + Sbjct: 270 TSRLLRMPMGRAWEALREDEIACRSLGINPTKVKLSAFTLGAAFAGLAGSFFAARQGLVT 329 Query: 220 PASVLGTGMSIKMALVAILGGIGTLFGPLVGA 251 P S ++ +A+V +LGG+G+ G ++ A Sbjct: 330 PESFTFIESALILAIV-VLGGMGSQLGVILAA 360 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.141 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,085,135 Number of extensions: 46278 Number of successful extensions: 485 Number of sequences better than 1.0e-08: 43 Number of HSP's gapped: 458 Number of HSP's successfully gapped: 47 Length of query: 344 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 258 Effective length of database: 1,212,660 Effective search space: 312866280 Effective search space used: 312866280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 131 (55.1 bits)