BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0071.p (197 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312008|ref|YP_003776100.1| RNA polymerase sigma-E factor [... 131 3e-32 gi|300312009|ref|YP_003776101.1| hypothetical protein Hsero_2697... 70 2e-13 >gi|300312008|ref|YP_003776100.1| RNA polymerase sigma-E factor [Herbaspirillum seropedicae SmR1] Length = 163 Score = 131 bits (330), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 84/129 (65%), Positives = 97/129 (75%), Gaps = 3/129 (2%) Query: 72 QKLASWRRGSDMRPWLFSIMHNLHVDQRRKPVIPTVALDDEDALATPAYAPDRLVGYDLE 131 QKL SW+RGS MRPWLFSIMHNLH D RR+ TVALD+ + A D V DLE Sbjct: 35 QKLDSWQRGSPMRPWLFSIMHNLHADARRRASPATVALDEYGEIDQAQVALDPGVLLDLE 94 Query: 132 SALRLLAAEQREILLLVVLEEMTYEEVAATLGIPLGTVMSRLSRARERLRTLVEGRPQH- 190 +AL+LL EQRE+LLLVVLEEM YEEVA TLGIP GTVMSRLSRARERLR +++G+ + Sbjct: 95 TALQLLPPEQREVLLLVVLEEMRYEEVAVTLGIPTGTVMSRLSRARERLRRVMQGQGESS 154 Query: 191 --STLKVVK 197 S+LKVVK Sbjct: 155 PGSSLKVVK 163 >gi|300312009|ref|YP_003776101.1| hypothetical protein Hsero_2697 [Herbaspirillum seropedicae SmR1] Length = 123 Score = 69.7 bits (169), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 36/64 (56%), Positives = 40/64 (62%), Gaps = 2/64 (3%) Query: 16 SAVPAAFAEEMAVKKADDVLVTTKGMTVYTFDND--PVGKSVCNGPCATAWPPVAPVGQK 73 SA A A+ VK D +LV GMTVYTFD D GKSVCNGPCA WPPVAP+G Sbjct: 15 SASVTALAQGAGVKAMDGILVDAHGMTVYTFDKDTPNNGKSVCNGPCAKLWPPVAPMGSP 74 Query: 74 LASW 77 A + Sbjct: 75 AAPY 78 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 569,890 Number of extensions: 22847 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 2 Length of query: 197 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 117 Effective length of database: 1,241,070 Effective search space: 145205190 Effective search space used: 145205190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)