BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0068.p (84 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane... 131 8e-33 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 70 4e-14 gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709... 67 3e-13 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 64 3e-12 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 63 4e-12 >gi|300312012|ref|YP_003776104.1| phosphodiesterase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 797 Score = 131 bits (330), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 59/84 (70%), Positives = 66/84 (78%) Query: 1 MNMEHDADDAKIVKSTIDLGHNLGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMPG 60 M M D DDAKIV+ST+DLGHNLGLRVVAEG+E+ VW LLRQMGCDQ QGYFM KPMP Sbjct: 714 MKMAQDQDDAKIVRSTVDLGHNLGLRVVAEGVESEEVWFLLRQMGCDQGQGYFMGKPMPV 773 Query: 61 DRFIEWLEQWTPPEMPSQSENMAI 84 F+ W EQW P+M QSENMA+ Sbjct: 774 QHFLAWREQWRAPDMLDQSENMAV 797 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 69.7 bits (169), Expect = 4e-14, Method: Composition-based stats. Identities = 33/64 (51%), Positives = 41/64 (64%) Query: 6 DADDAKIVKSTIDLGHNLGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMPGDRFIE 65 DADDA I S I L +L LRV+AEG+ET + LR GCD+ QGY S P+P D+F + Sbjct: 880 DADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSPPVPADKFEQ 939 Query: 66 WLEQ 69 L Q Sbjct: 940 ILRQ 943 >gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709 [Herbaspirillum seropedicae SmR1] Length = 598 Score = 67.0 bits (162), Expect = 3e-13, Method: Composition-based stats. Identities = 32/64 (50%), Positives = 39/64 (60%) Query: 6 DADDAKIVKSTIDLGHNLGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMPGDRFIE 65 D D IV S + L HNLG VVAEGLE+ LLR +GCD QGY +++PMP Sbjct: 533 DEADQLIVPSILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARPMPLAELEA 592 Query: 66 WLEQ 69 WLE+ Sbjct: 593 WLER 596 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 63.5 bits (153), Expect = 3e-12, Method: Composition-based stats. Identities = 29/62 (46%), Positives = 42/62 (67%), Gaps = 1/62 (1%) Query: 6 DADDAK-IVKSTIDLGHNLGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMPGDRFI 64 DAD K IV++ I + H LG++V+AEG+ET+ LLR GC+ QGY S+P+P D F+ Sbjct: 675 DADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQGYLYSRPLPEDDFL 734 Query: 65 EW 66 + Sbjct: 735 NF 736 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 63.2 bits (152), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 29/68 (42%), Positives = 40/68 (58%) Query: 2 NMEHDADDAKIVKSTIDLGHNLGLRVVAEGLETLAVWNLLRQMGCDQAQGYFMSKPMPGD 61 ++ DAD A IVK+ I L H L LRV+AEG+E L++ CD+ QGY+ KPM Sbjct: 623 DIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKPMEVA 682 Query: 62 RFIEWLEQ 69 F W+ + Sbjct: 683 VFTAWMSE 690 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 289,474 Number of extensions: 10071 Number of successful extensions: 54 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 54 Number of HSP's successfully gapped: 13 Length of query: 84 Length of database: 1,619,870 Length adjustment: 54 Effective length of query: 30 Effective length of database: 1,364,180 Effective search space: 40925400 Effective search space used: 40925400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)