BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0059.p (272 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 209 2e-55 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 101 8e-23 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 67 2e-12 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 65 6e-12 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 59 3e-10 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 209 bits (531), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 107/119 (89%), Positives = 111/119 (93%), Gaps = 3/119 (2%) Query: 1 MASSP---QTYTPPPPLTGGKLVLGTLCVSLAVFMNVLDSSIANVAIPTISGNLGVSIDE 57 MA+SP Q YTPPPPLTG LVLGTL VSLAVFMNVLDSSIANVAIPTISGNLGVS+DE Sbjct: 1 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 Query: 58 GTWVITSFAAANAISIPLTGWLTQRIGAVRLFVSSVLLFVLASWLCGLAPTLPILLGAR 116 GTWVITSFAAANAISIPLTGWLTQR+GAVRLFV+SVLLFVLASWLCGLAPTLPILL AR Sbjct: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAAR 119 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 101 bits (251), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 52/103 (50%), Positives = 77/103 (74%) Query: 14 LTGGKLVLGTLCVSLAVFMNVLDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISI 73 L+GG+LV + ++ A F+ VLD++IANV++ TI+G LG S +GT+VITS+A A AI++ Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 74 PLTGWLTQRIGAVRLFVSSVLLFVLASWLCGLAPTLPILLGAR 116 PLTGWL R G VR+F+ S+++F L S LCG+A +L +L+ R Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFR 128 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 37/90 (41%), Positives = 56/90 (62%) Query: 27 SLAVFMNVLDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISIPLTGWLTQRIGAV 86 +L M LD SI N ++PTI G +G S EGTW+ T++ A + IP++ WL + +G Sbjct: 23 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLR 82 Query: 87 RLFVSSVLLFVLASWLCGLAPTLPILLGAR 116 L +++ LF L S LCGL+ TLP+++ R Sbjct: 83 VLLLTAASLFTLFSVLCGLSTTLPMMIVGR 112 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 65.1 bits (157), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 34/92 (36%), Positives = 54/92 (58%) Query: 25 CVSLAVFMNVLDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISIPLTGWLTQRIG 84 V+ FM LD++I N A+P ++ +LG + V+ ++ A P +GWL R G Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 85 AVRLFVSSVLLFVLASWLCGLAPTLPILLGAR 116 R++ +++LLF L S LC +A +LP+LL AR Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAAR 148 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 59.3 bits (142), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 32/93 (34%), Positives = 53/93 (56%) Query: 24 LCVSLAVFMNVLDSSIANVAIPTISGNLGVSIDEGTWVITSFAAANAISIPLTGWLTQRI 83 + +++ M +LD ++ NV + I+G+L + W++ + A + + G L R Sbjct: 33 VALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADRF 92 Query: 84 GAVRLFVSSVLLFVLASWLCGLAPTLPILLGAR 116 GA RL+++ + LFVLAS LCG AP P L+ AR Sbjct: 93 GARRLYLTGLFLFVLASLLCGAAPNGPFLIVAR 125 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 696,981 Number of extensions: 23597 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 10 Length of query: 272 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 188 Effective length of database: 1,222,130 Effective search space: 229760440 Effective search space used: 229760440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)