BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0053.p (344 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 463 e-132 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 160 1e-40 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 85 8e-18 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 80 3e-16 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 71 1e-13 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 463 bits (1192), Expect = e-132, Method: Compositional matrix adjust. Identities = 230/344 (66%), Positives = 277/344 (80%) Query: 1 MKALVLEKQHDIQLREIDLPLAIGPRDVKIKIHTVGVCGSDVHYYQHGNIGSFVVNEPMV 60 M+ALVLE +++LREIDLP +G +DV+I+IHTVG+CGSD+HYY HG+IG F V PMV Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 61 LGHEAAGVVAEVGAEVRNLKVGDRVCMEPGVPDFESTATRLGLYNLDPKVRFWATPPVHG 120 LGHEA+G V EVG+ V +LKVGDRVCMEPG+P +S AT G+YNLDP VRFWATPP+HG Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHG 120 Query: 121 CLTPFVSHPAAFTYKLPDNVSFAEGAIVEPLAIGLQAATKARIKPGDLAVVIGAGTIGMM 180 CLT V HPAAFTY+LPDNVSFAEGAIVEPL+IGLQAATKAR+KPGD AVVIGAGTIG M Sbjct: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 Query: 181 TALAALAGGCSGVVICDLVQEKLDVIDPYPGITTVNLRRQSLPEMVAGISSGWGADIVYE 240 TALAALAGG + V++ D+V EKL P + TV++ R++L ++V + GWGAD+V+E Sbjct: 181 TALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGWGADVVFE 240 Query: 241 ASGSDKAYDGIFDLLCPGGCLVLVGMPNTKVAIDIVAIQTKEIRIESVFRYANIFSRALA 300 ASG Y + DL+CPGGC VLVGMP VA+D+VA+QTKE+R+ESVFRYANIF RALA Sbjct: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALA 300 Query: 301 LIASGKINVKPFISRSFKFADGVAAFEAAAGADPHDVKIQIELE 344 LI+SG I+VKPFISR F F+ + AFE AA P DVKIQIE+E Sbjct: 301 LISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEME 344 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 106/348 (30%), Positives = 174/348 (50%), Gaps = 10/348 (2%) Query: 2 KALVLEKQHDIQLREIDLPLAIGPRDVKIKIHTVGVCGSDVHYYQHGNIGSFVVNEPMVL 61 + LV+ HD++++ +D A+ P+ +K+K+ G+CGSD+HYY HG G + EPMVL Sbjct: 3 QGLVIHAPHDLRIQALDTG-ALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVL 61 Query: 62 GHEAAGVVAEVGAEVRNLKVGDRVCMEPGVPDFESTATRLGLYNLDPKVRFWA----TPP 117 GHE +G +AEVG V ++ G R+ + P P + G N +RF+ TP Sbjct: 62 GHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPH 121 Query: 118 VHGCLTPFVSHPAAFTYKLPDNVSFAEGAIVEPLAIGLQAATKARIKPGDLAVVIGAGTI 177 V G + + + + D+VS E A+ EPL++ L A +A G +V G G I Sbjct: 122 VQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPI 181 Query: 178 GMMTALAALAGGCSGVVICDLVQEKLDVIDPYPGITTVNLRRQSLPEMVAGISSGWGA-D 236 G + +AA G VV+ D+ L + +N+ P + ++ G D Sbjct: 182 GALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINM--AETPAALKAYAADKGTFD 239 Query: 237 IVYEASGSDKAYDGIFDLLCPGGCLVLVGMPNTKVAIDIVAIQTKEIRIESVFRYANIFS 296 +++EASG+++A G + L P +V VG+ + + KE+ FR+ F+ Sbjct: 240 VLFEASGNERALVGALEALRPQAIIVQVGL-GGDIQFPANLLVGKELEWRGAFRFHEEFA 298 Query: 297 RALALIASGKINVKPFISRSFKFADGVAAFEAAAGADPHDVKIQIELE 344 A+ L+ ++VKP I+ ++ A V AFE AG +K+QI + Sbjct: 299 MAVELMNRKLVDVKPLITATYPIAQAVEAFE-IAGDRSRMMKVQISFD 345 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 85.1 bits (209), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 96/369 (26%), Positives = 153/369 (41%), Gaps = 41/369 (11%) Query: 1 MKALVLEKQHDIQLREIDLPLAI--------GPRDVKIKIHTVGVCGSDVHYYQHGNIGS 52 ++A VL + D + PL I GP +V +++ G+C SD+ G Sbjct: 3 IQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVI----TGV 58 Query: 53 FVVNEPMVLGHEAAGVVAEVGAEVRNLKVGDRV-------------CME--PGVPDFEST 97 PM LGHEA+ V EVG+ V +L+ GD V CME P + + + Sbjct: 59 RPRPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAA 118 Query: 98 ATRLGLYNLDPKVRFWATPPVHGCL--TPFVSHPAAFTY---KLPDNVSFAEGAIVE-PL 151 +G + T PVH L + F H K+ ++ + A+ + Sbjct: 119 TNTVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAV 178 Query: 152 AIGLQAATK-ARIKPGDLAVVIGAGTIGMMTALAALAGGCSGVVICDLVQEKLDVIDPYP 210 G+ AA A +K G A V+G G +G+ L ALA G V+ DL KL + Sbjct: 179 LTGVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLG 238 Query: 211 GITTVNLRRQSLPEMVAGISSGWGADIVYEASGSDKAYDGIFDLLCPGGCLVLVGM--PN 268 TVN R E V ++ G G D +E +GS +A + + + GG V G+ P Sbjct: 239 ATATVNARDPDAVEKVRALTRG-GVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPG 297 Query: 269 TKVAIDIVAIQTKEIRIESVFRYANI----FSRALALIASGKINVKPFISRSFKFADGVA 324 A+ V++ +E ++ + + + R + L +GK+ V + AD Sbjct: 298 QHWALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINR 357 Query: 325 AFEAAAGAD 333 F+ G + Sbjct: 358 GFDRLHGGE 366 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 79.7 bits (195), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 86/309 (27%), Positives = 129/309 (41%), Gaps = 25/309 (8%) Query: 14 LREIDLPLAI--------GPRDVKIKIHTVGVCGSDVHYYQHGNIGSFVVNEPMVLGHEA 65 +RE PL+I P + +K GVC +D+H HG+ P + GHE Sbjct: 10 VREFGKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHA-AHGDW-PVKPTPPFIPGHEG 67 Query: 66 AGVVAEVGAEVRNLKVGDRVCMEPGVPDFES-----TATRLGLYNLDPKVRFWATPPVHG 120 G VA VGA V+++K GDRV GVP + + R G L + + V+G Sbjct: 68 TGYVAAVGAGVKHVKEGDRV----GVPWLHTACGCCSPCRTGWETLCAEQQNTGY-SVNG 122 Query: 121 CLTPFVSHPAAFTYKLPDNVSFAEGAIVEPLAIGLQAATK-ARIKPGDLAVVIGAGTIGM 179 + F LPDN+ F A V + + K ++PG+ V+ G G +G Sbjct: 123 SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGLGH 182 Query: 180 MTALAALAGGCSGVVICDLVQEKLDVIDPYPGITTVNLRRQSLPEMVAGISSGWGADIVY 239 M A A G VV D+ ++KL + TV+ R + V I G +V Sbjct: 183 MAVQYAKAMGMH-VVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAHGALVT 241 Query: 240 EASGSDKAYDGIFDLLCPGGCLVLVGMPNTKVAIDIVAIQTKEIRIE-SVFRYANIFSRA 298 S KA + F L G + LVG+P +++ + K I + S+ + Sbjct: 242 AV--SPKAMEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRITVRGSIVGTRQDLEES 299 Query: 299 LALIASGKI 307 L A GK+ Sbjct: 300 LVFAAEGKV 308 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 72/277 (25%), Positives = 113/277 (40%), Gaps = 47/277 (16%) Query: 1 MKALVLEKQHDIQLREIDLPLAIGPRDVKIKIHTVGVCGSDVHYYQHGNIGSFVVNEPMV 60 M+AL+ HD+++ + P+ DV +K+ +CGSD+H Y+ G I + + + Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLYR-GKIPATKAGD--I 57 Query: 61 LGHEAAGVVAEVGAEVRNLKVGDRVCM--------------------EPGVPD----FES 96 LGHE GVV E G ++ LK GDRV + E PD + Sbjct: 58 LGHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNA 117 Query: 97 TATRLGLYNLDPKVRFWATPPVHGCLTPFVSHPAAFTYKLPDNVSFAEGAIV---EPLAI 153 R G + P G +V P A + + + A+ ++ + L Sbjct: 118 KGIRSGAAMFGYSHLYGGMP---GGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPT 174 Query: 154 GLQAATKARIKPGDLAVVIGAGTIGMMTALAALAGGCSGVVICD-------LVQEKLDVI 206 G QA A ++ G + GAG +G M A +A G + + D QE+ DVI Sbjct: 175 GYQAVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVI 234 Query: 207 DPYPGITTVNLRRQSLPEMVAGISSGWGADIVYEASG 243 VN + E + ++G G D V +A G Sbjct: 235 -------PVNFDQVDPAEYIIANTAGRGVDGVIDAVG 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 899,051 Number of extensions: 33229 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 5 Length of query: 344 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 258 Effective length of database: 1,212,660 Effective search space: 312866280 Effective search space used: 312866280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)