BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0038.p (566 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 620 e-179 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 131 1e-31 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 124 3e-29 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 102 8e-23 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 94 2e-20 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 620 bits (1599), Expect = e-179, Method: Compositional matrix adjust. Identities = 297/349 (85%), Positives = 322/349 (92%) Query: 1 MQYRPLGRTNINVSLITLGTMTWGEQNTEAEAHSQLDYAVERGINLIDVAEMYPVPPKAE 60 MQYRPLGRT++ VSLITLGTMTWGEQN+EA+AH+QLDYAVERGINLIDVAEMYPVPPKAE Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 61 TQGLSERYLGTWLKKSGKRDQLLIATKATGPARKPHNPRHVRGGINNLDRKGLTDALNGS 120 TQGL+ERYLGTWLKKSGKRDQLLIATK TGPARKPHNPRHVRGGINNLDRKGLT+AL+GS Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS 120 Query: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLNYAHVADEETVAIDETLRVLSEFVKAGKIRHIG 180 LERLQLDHVDLYQLHWPDRSVNSFGQL Y HV DE TV I+ETL VLSEFVKAGKIR IG Sbjct: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIG 180 Query: 181 LSNETAWGVGQFLRAAEKFDLERVVSIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 LSNETAWGV QFLRAAE+ LER+V+IQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA Sbjct: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 Query: 241 MGMLSGKYENGARPAAGRLTIFERFTRYSNPQAQTATTAYVALARKHGLDPAQLALAYVN 300 GMLSGKY +GARPA GRLT+FERF+RY+N QA+ AT YVALAR+HGLDPAQ+ALAYVN Sbjct: 241 FGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALAYVN 300 Query: 301 SRPFVTSNIIGATTLEQLRSNIDSLSLQLSPEIIEQIDAIHKTQPNPAP 349 SRPF+TSNIIGATTL QLRSNIDS LQL +++ I+AIH+ QPNPAP Sbjct: 301 SRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAIHRRQPNPAP 349 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 131 bits (329), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 98/349 (28%), Positives = 168/349 (48%), Gaps = 48/349 (13%) Query: 1 MQYRPLGRTNINVSLITLGTMTWGEQNTEAEAHSQLDYAVERGINLIDVAEMYPVPPKAE 60 M YR LG++N+ VS + LG+M +G+Q E+ + A E G+N ID A++Y Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVY------- 53 Query: 61 TQGLSERYLGTWLKKSGKRDQLLIATK-ATGPARKPHNPRHVRGGINNLDRKGLTDALNG 119 T+G SE +G L +G+R + ++A+K + KP+ +N R + Sbjct: 54 TKGKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNE--------SNYSRSWIVRETEN 103 Query: 120 SLERLQLDHVDLYQLHWPDRSVNSFGQLNYAHVADEETVAIDETLRVLSEFVKAGKIRHI 179 L RL D++D+ +H D + ++E ++ L + ++ GKIR Sbjct: 104 ILTRLDTDYLDILYMH-----------------RDYQDENLEEAVQALGDLIRVGKIRGF 146 Query: 180 GLSNETAWGVGQFLRAAEKFDLERVVSIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPL 239 G+SN W + + +R EK ++ + Q YNLLNR E+ + +G++ YSP+ Sbjct: 147 GVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPI 206 Query: 240 AMGMLSGKYENGARPA----AGRLTIFERFTRYSNPQAQTATTAYVALARKH----GLDP 291 A G+L+GKY+ G +PA AGR R + + + ++H G+ Sbjct: 207 ARGVLTGKYQPGQKPAQDTRAGRGD-----KRMMETEFREESLLIAQRLQQHCEARGVKL 261 Query: 292 AQLALAYVNSRPFVTSNIIGATTLEQLRSNIDSLSLQLSPEIIEQIDAI 340 Q A A+V + ++S I G TL Q+ D+ L++S E +D++ Sbjct: 262 GQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKISAEEEALVDSL 310 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 124 bits (310), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 114/353 (32%), Positives = 170/353 (48%), Gaps = 64/353 (18%) Query: 4 RPLGRTNINVSLITLGTMTWGE---QNTEAEAHSQLDYAVERGINLIDVAEMYPVPPKAE 60 R LG VS I LG M + Q +AE+ + +D A+E GINL+D A+MY Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMY------- 70 Query: 61 TQGLSERYLGTWLKKSGKRDQLLIATKATGPARKPHNP--RHVRGGINNLDRKGLTDALN 118 +E LG +K G+RD+ IATK G R P +P R V G + + A+ Sbjct: 71 GPYTNEELLGRAIK--GRRDKFFIATK-FGIRRDPSDPGARGVDGSPAYIRQ-----AVE 122 Query: 119 GSLERLQLDHVDLYQLHWPDRSVNSFGQLNYAHVADEETVAIDETLRVLSEFVKAGKIRH 178 GSL+RL ++ +DLY Y H D T I+ T+ VL++ V+AGKIR+ Sbjct: 123 GSLKRLGVETIDLY----------------YQHRIDPAT-PIEVTMGVLADLVQAGKIRY 165 Query: 179 IGLSNETAWGVGQFLRAAEKFDLERVVSIQNPYNLLNRSFEINLAEFAHREQVGLLAYSP 238 IGLS +A + + R + V ++Q+ Y+L R E + +G +AYSP Sbjct: 166 IGLSEASAATLERAHR------VHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSP 219 Query: 239 LAMGMLSG---KYENGARPAAGRLTIFERFTRYSNPQAQTATTA--------YVALARKH 287 L G L+G ++E+ + E R SNP+ Q A +A +H Sbjct: 220 LGRGFLTGAIQRFED----------LAEDDFRRSNPRFQGENFARNLQLAEKVKEMAGQH 269 Query: 288 GLDPAQLALAYVNSRPFVTSNIIGATTLEQLRSNIDSLSLQLSPEIIEQIDAI 340 G P+QLALA+V ++ I G L N S+ ++L+PE ++ +DAI Sbjct: 270 GCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQALDAI 322 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 102 bits (254), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 101/351 (28%), Positives = 160/351 (45%), Gaps = 51/351 (14%) Query: 1 MQYRPLGRTNINVSLITLGTM-TWGEQNTEAEAHSQLDYAVERGINLIDVAEMYPVPPKA 59 M YR GR+ + + I+LG +G + + L A +RG+ D+A Y P Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPP--- 70 Query: 60 ETQGLSERYLGTWLKKS--GKRDQLLIATKA-----TGPARKPHNPRHVRGGINNLDRKG 112 G +E G W+ K RD+L+I++KA GP P R K Sbjct: 71 --AGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGAR-----------KH 117 Query: 113 LTDALNGSLERLQLDHVDLYQLHWPDRSVNSFGQLNYAHVADEETVAIDETLRVLSEFVK 172 L + SL+R+ LD+VD++ Y+H D +T ++ET+ L+ + Sbjct: 118 LIASCEQSLKRMGLDYVDIF----------------YSHRVDADT-PLEETMGALAHLHR 160 Query: 173 AGKIRHIGLSNETAWGVGQFLRAAEKFDLERVVSI---QNPYNLLNRSFEINLAEFAHRE 229 GK ++G+S+ + + R A V + Q Y++LNR+ E L Sbjct: 161 QGKALYVGISSYSP----ELTRKAAAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEEL 216 Query: 230 QVGLLAYSPLAMGMLSGKYENGARPAAGRLTIFERFTRYSNPQAQTA-TTAYVALARKHG 288 VG + +SPLA G+L+ KY G P R+T + + A A +A K G Sbjct: 217 GVGCIGFSPLAQGLLTSKYLGGV-PEGARVTRAPSLPQRMLSEDNLARIRALNEIASKRG 275 Query: 289 LDPAQLALAYVNSRPFVTSNIIGATTLEQLRSNIDSLS-LQLSPEIIEQID 338 AQ+A+A+V VTS +IGA T+EQL ++D+++ L + E + ID Sbjct: 276 QTLAQMAIAWVLRDARVTSALIGARTVEQLDDSLDAVNKLDFTQEELASID 326 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 94.4 bits (233), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 107/356 (30%), Positives = 165/356 (46%), Gaps = 54/356 (15%) Query: 1 MQYRPLGRTNINVSLITLGTM----TWGEQNTEAEAHSQLDYAVERGINLIDVAEMYPVP 56 M+ R GRT VS I G +WG+ + + +A + L A++ G++ ID A++Y Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQ-DAKAALHAALDAGVSFIDTADVY--- 56 Query: 57 PKAETQGLSERYLGTWLKKSGKRDQLLIATKATGPARKPHNPRHVRGGINNLDRKGLTDA 116 G SE+ + LK+ G ++ +ATKA G PH V G N DR L+ Sbjct: 57 ----GDGRSEQLIAQVLKERGG-ERPFVATKA-GRRLSPH----VASGYN--DRAQLSAF 104 Query: 117 LNGSLERLQLDHVDLYQLHWPDRSVNSFGQLNYAHVADEETVAIDETLRVLSEFVKAGKI 176 ++ SL+ L +D +DL QLH P V + Q + H L V AGKI Sbjct: 105 IDRSLKNLGVDCLDLVQLHCPPTEVY-YQQEVFGH---------------LDALVAAGKI 148 Query: 177 RHIGLSNETAWGVGQFLRAAEKFDLERVVSIQNPYNLL-NRSFEINLAEFAHREQVGLLA 235 RH G+S E V + L+A E V S+Q +N+ R + L E A R + ++A Sbjct: 149 RHYGVSVEK---VEEALKAIE---YPNVKSVQIIFNIFRQRPISLFLQE-AQRRDIAVIA 201 Query: 236 YSPLAMGMLSGKYENGARPAAGRLTIFERFTRYSNPQAQTATTAY-VALAR--------K 286 PLA G+L+GK AA F R + + Y VALA Sbjct: 202 RVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRALVP 261 Query: 287 HGLDPAQLALAYVNSRPFVTSNIIGATTLEQLRSNIDSLSL-QLSPEIIEQIDAIH 341 G+ AQ AL ++ +++ I GA + Q +SN + +L ++ E+++ + AI+ Sbjct: 262 QGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASALPPITAEVMQALRAIY 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,679,585 Number of extensions: 71778 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 6 Length of query: 566 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 476 Effective length of database: 1,193,720 Effective search space: 568210720 Effective search space used: 568210720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)