BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0030.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 692 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 181 8e-47 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 172 4e-44 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 159 4e-40 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 102 6e-23 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 692 bits (1785), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/382 (89%), Positives = 368/382 (96%) Query: 5 SDAYQDLRDAVRDLCNTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLG 64 SD++ DLRDAVRDLC TFPPEYWRK+DEARGYPE+FVDALTKAGWLAALIP EYGGSGLG Sbjct: 7 SDSHADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLG 66 Query: 65 LTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSEQQKQLYLPKIASGELRLQSMGVT 124 LTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGS +QK+ YLP+IASGELRLQSM VT Sbjct: 67 LTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRLQSMAVT 126 Query: 125 EPTTGTDTTKIKTTAVKKGDRYVVNGQKVWISRIQHSDLMILLARTTPLDQVKKKSEGMS 184 EPTTGTDTT+IKT AVKKGDRYV+NGQKVWISR+QHSDLMILLARTTPL+QV +KSEGMS Sbjct: 127 EPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMS 186 Query: 185 IFLVDLKQAIGNGMSVRPILNMVNHETNELFFDNLEIPVENLIGEEGKGFKYILDGLNAE 244 IFLVDLK+AIG+GM VRPILNMVNHETNELFFDNLEIP ENLIGEEG+GF+YILDGLNAE Sbjct: 187 IFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAE 246 Query: 245 RTLIAAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSYIELEAANLMRFKA 304 RTLIAAECIGDGYWFI+KA QYA+ERVVFDRPIGMNQGVQFP+A++YIE+EAANLMR++A Sbjct: 247 RTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAANLMRYQA 306 Query: 305 CELFDAHQPCGAQANMAKFLAAKASWEAANVCLQTHGGFGFACEYDVERKFRETRLYQVA 364 C LFDA Q CGAQANMAK+LAAKA+WEAANVCLQTHGGFGFACEYDVERKFRETRLYQVA Sbjct: 307 CALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRETRLYQVA 366 Query: 365 PISTNLIYSYVAEHILGLPRSF 386 PISTNLI+SYVAEH+LGLPRSF Sbjct: 367 PISTNLIFSYVAEHVLGLPRSF 388 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 181 bits (459), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 121/371 (32%), Positives = 194/371 (52%), Gaps = 27/371 (7%) Query: 5 SDAYQDLRDAVRDLCNTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLG 64 S ++ +RDA+R + D +P E + L + G + +P ++GG+G+ Sbjct: 4 SPEHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMD 63 Query: 65 LTEASVIMEEINRTGG---------NSGACHGQMYNMGTLLRHGSEQQKQLYLPKIASGE 115 + +EEI G NS AC G ++GS QK+ +L +A GE Sbjct: 64 YMSLVLALEEIAAGDGATSTIVSVQNSLAC-------GITEKYGSPTQKEQWLKPLARGE 116 Query: 116 LRLQSMGVTEPTTGTDTTKIKTTAVKKGDRYVVNGQKVWISRIQHSDLMILLARTTPLDQ 175 + L +TEP TG+D I+T A + GD +++NG K +I+ +H+ + I+ A T D+ Sbjct: 117 M-LGCFCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVT---DR 172 Query: 176 VKKKSEGMSIFLVDLKQAIGNGMSV-RPILNMVNH--ETNELFFDNLEIPVENLIGEEGK 232 K +G+S FLV G V R M H +T ++ DN +P L+G+EG+ Sbjct: 173 SAGK-KGISCFLVPCDTP---GFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGE 228 Query: 233 GFKYILDGLNAERTLIAAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSYI 292 G++ L L A R IAA+ +G E A YA++R F PI +Q V F +AD Sbjct: 229 GYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNT 288 Query: 293 ELEAANLMRFKACELFDAHQPCGAQANMAKFLAAKASWEAANVCLQTHGGFGFACEYDVE 352 L+AA LM ++A +L D +PC +A+MAK A++A+ + A+ +Q HGG G+ ++ VE Sbjct: 289 LLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVE 348 Query: 353 RKFRETRLYQV 363 R +R+ R+ Q+ Sbjct: 349 RIYRDVRICQI 359 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 172 bits (436), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 113/372 (30%), Positives = 195/372 (52%), Gaps = 21/372 (5%) Query: 11 LRDAVRDLCNTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLGLTEASV 70 LR+AV ++ ++D + +P + L G L + EYGG+GLG + Sbjct: 18 LREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHII 77 Query: 71 IMEEINRTGGNSGACHGQMYNM--GTLLRHGSEQQKQLYLPKIASGELRLQSMGVTEPTT 128 MEEI+R + G +G N+ + R+G+E+QK+ YLP++ SG+ + ++ ++EP Sbjct: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDF-IGALAMSEPNA 136 Query: 129 GTDTTKIKTTAVKKGDRYVVNGQKVWISRIQHSDLMILLARTTPLDQVKKKSEGMSIFLV 188 G+D +K A KKGDRYV+NG K+WI+ +D++++ A+T ++ + GM+ FLV Sbjct: 137 GSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKT----DLEAGARGMTAFLV 192 Query: 189 DLKQAIGNGMSVRPILN---MVNHETNELFFDNLEIPVENLIGEEGKGFKYILDGLNAER 245 + G SV L+ M T EL F + E+P EN++G G+G ++ GL+ ER Sbjct: 193 EKGY---KGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFER 249 Query: 246 TLIAAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSYIELEAANLMRF--- 302 ++++ +G ++ Y +R F + IG Q +Q +AD Y + A + Sbjct: 250 SVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVG 309 Query: 303 KACELFDAHQPCGA----QANMAKFLAAKASWEAANVCLQTHGGFGFACEYDVERKFRET 358 +AC+ D+ A A + A KA+W A +Q+ GG G+ EY V R +R+ Sbjct: 310 QACDRADSADKVRALRKDAAGAILYSAEKATWMAGE-AIQSLGGNGYINEYPVGRLWRDA 368 Query: 359 RLYQVAPISTNL 370 +LY++ ++ + Sbjct: 369 KLYEIGAGTSEI 380 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 159 bits (402), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 113/378 (29%), Positives = 186/378 (49%), Gaps = 20/378 (5%) Query: 11 LRDAVRDLCNTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLGLTEASV 70 + + RD + K DE +P + + + G+ P GG GL +A+V Sbjct: 12 FQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATV 71 Query: 71 IMEEINRTGGNSGACHGQMYNMGTLL--RHGSEQQKQLYLPKIASGELRLQSMGVTEPTT 128 + EE+ R S A + ++NM + + G K + ++A+G ++ S +TEP + Sbjct: 72 VFEELARAC-TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGR-KIGSYCLTEPGS 129 Query: 129 GTDTTKIKTTAVKKGDRYVVNGQKVWISRIQHSDLMILLARTTPLDQVKKKSEGMSIFLV 188 G+D +KTTA + YV+NG K +IS +D+++++ART + + G+S +V Sbjct: 130 GSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-----GEGARGVSAIVV 184 Query: 189 DLKQAIGNGMSV-----RPILNMVNHETNELFFDNLEIPVENLIGEEGKGFKYILDGLNA 243 GN V + + T + FDN+++P ++L+G+EG+GF + + GL+ Sbjct: 185 P-----GNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDG 239 Query: 244 ERTLIAAECIGDGYWFIEKAAQYAKERVVFDRPIGMNQGVQFPIADSYIELEAANLMRFK 303 R IA +G ++ A Y KER F RPI Q +QF +AD EL AA M Sbjct: 240 GRINIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRL 299 Query: 304 ACELFDAHQP-CGAQANMAKFLAAKASWEAANVCLQTHGGFGFACEYDVERKFRETRLYQ 362 A DA P MAK LA + N LQ HGG+G+ EY +ER FR+ R++Q Sbjct: 300 AATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQ 359 Query: 363 VAPISTNLIYSYVAEHIL 380 + + ++ ++ +L Sbjct: 360 ILEGTNEIMRVIISRKLL 377 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 102 bits (253), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 80/315 (25%), Positives = 145/315 (46%), Gaps = 28/315 (8%) Query: 13 DAVRDLCNTFPPEYWRKVDEARGYPEEFVDALTKAGWLAALIPAEYGGSGLGLTEASVIM 72 D +R + T P K DE F A+ AG++ AL+P +GG+ L + ++ Sbjct: 24 DVLRKVGPTITP--LVKPDERFYATRPFYQAMADAGFIKALLPESFGGTALSTLDFALAA 81 Query: 73 EEINRTGGN-SGACHGQMYNMGTLLRHGSEQQKQLYLPKIASGELRLQSMGVTEPTTGTD 131 EE+ N A G + ++ HG+ +QKQ +LP RL ++ TE T G + Sbjct: 82 EELTAVDVNVPSALLGTGLGLHAVVHHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGAN 141 Query: 132 ------TTKIKTTAVKKGDRYVVNGQKVWISRIQHSD-----LMILLARTTPLDQVKKKS 180 + ++T A +GD +V+NG K + + D L ++ RT P Sbjct: 142 YDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGKGAHLYSVICRTDP---ALPPQ 198 Query: 181 EGMSIFLVDLKQAIGN--GMSVRPILNMVNHETN---ELFFDNLEIPVENLIGEEGKGFK 235 + +++ +V GN G+ + +L+ + H + F+N+ +P N++G+ G G Sbjct: 199 QSLAVIMVP-----GNAPGIEIVGLLDTMGHRATISPRVHFNNVRVPASNIVGKPGDGIS 253 Query: 236 YILDGLNAERTLIAAECIGDGYWFIEKAAQYAK-ERVVFDRPIGMNQGVQFPIADSYIEL 294 + + LI + C+G + A ++A+ +R P+ +Q V F +AD + + Sbjct: 254 MLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSGTIPVIEHQNVGFMLADIKMRI 313 Query: 295 EAANLMRFKACELFD 309 EAA + +K+C D Sbjct: 314 EAARYLTWKSCHQLD 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,167,734 Number of extensions: 47174 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 10 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)