BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0022.p (133 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312112|ref|YP_003776204.1| transmembrane permease [Herbasp... 185 9e-49 >gi|300312112|ref|YP_003776204.1| transmembrane permease [Herbaspirillum seropedicae SmR1] Length = 254 Score = 185 bits (470), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 95/118 (80%), Positives = 106/118 (89%) Query: 16 LPQKYIFLMGLAAGASAGFFSVGGAIVAVPGLVGLFGMTQTAAQGLGLAMVTPGTLVALW 75 L +++I +GL+AGASAGFFSVGGAIVAVP L FGMTQTAAQGLGLAMVTPGTLVALW Sbjct: 137 LGRRWIPFVGLSAGASAGFFSVGGAIVAVPLLTAFFGMTQTAAQGLGLAMVTPGTLVALW 196 Query: 76 TYASAGHVDWSVGIPLALGGVLSISWGVALAHHLPERRLRMLFCGALIAIAAWMMTHG 133 TYA AGHVDW+VGIP+ALGGVLSISWGVALAHHLPERRLR+LFCGAL+ ++ WMM G Sbjct: 197 TYAHAGHVDWAVGIPMALGGVLSISWGVALAHHLPERRLRILFCGALLLLSVWMMVGG 254 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 435,830 Number of extensions: 16946 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 106 Number of HSP's successfully gapped: 2 Length of query: 133 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 58 Effective length of database: 1,264,745 Effective search space: 73355210 Effective search space used: 73355210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)