BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0018.p (150 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 108 2e-25 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 60 1e-10 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 108 bits (269), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 58/131 (44%), Positives = 78/131 (59%), Gaps = 5/131 (3%) Query: 6 GRFFGQP-KDRGEVLSWLMFIATGLGPFSGQAVHFQHAAPAGLDYAVNRYRREIDRHYQV 64 GRF G+ +++ EVL W+MF GLGP GQA HF+ AP ++YAVNRY E R YQV Sbjct: 83 GRFLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLYAPEQIEYAVNRYTNEARRLYQV 142 Query: 65 LNQRLEGRTYIVGDECTVADISAWGWVDRAGHVFKKPEDPLGDYPNVKRWFQAMDARPAT 124 ++++L Y+ G+ T+ADI+ + W K L D+PNVKRW+ A+ ARPA Sbjct: 143 MDKQLGQHAYLAGEHYTIADIACFPWT----RSHKNQGIDLDDFPNVKRWYDAISARPAV 198 Query: 125 ARARKVGAXAR 135 R V A R Sbjct: 199 QRGVTVLADKR 209 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 59.7 bits (143), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 42/124 (33%), Positives = 64/124 (51%), Gaps = 9/124 (7%) Query: 15 RGEVLSWLMFIATGLGPFSGQAVHFQHAAPAGLDYAV----NRYRREIDRHYQVLNQRLE 70 R +V+ W+MF G+GP GQ F A G +Y +RY E R V+++RL Sbjct: 113 RYQVIQWVMFQMGGVGPMFGQLGFFHRFA--GKEYEDKRPRDRYVNESKRLLGVMDKRLA 170 Query: 71 GRTYIVGDECTVADISAWGWVDRAGHVFKKPEDPLG--DYPNVKRWFQAMDARPATARAR 128 ++ GD ++ADI+ + W+ R F + D +G D+P+V R +A ARPA R Sbjct: 171 QSQWLGGDAYSIADIATFPWI-RNLIGFYEAADLVGFQDFPHVARALEAFVARPAVVRGL 229 Query: 129 KVGA 132 + A Sbjct: 230 NIPA 233 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 492,475 Number of extensions: 20425 Number of successful extensions: 65 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 2 Length of query: 150 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 73 Effective length of database: 1,255,275 Effective search space: 91635075 Effective search space used: 91635075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)