BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0016.p (160 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 65 2e-12 gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum s... 59 2e-10 gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 55 2e-09 gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herb... 55 2e-09 gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein... 54 5e-09 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 65.5 bits (158), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 32/77 (41%), Positives = 45/77 (58%) Query: 1 MIEVNLIAPILLTRDVMPDLRASGDGMVVNVASGIALVGVPFYATYAAAKAGISHFGEAL 60 + E N + I +T+ +P +RA G G+V+NV S + L +P A Y A+KA I+ F E+L Sbjct: 95 LFETNTLGSIAMTQAFIPQMRARGQGLVINVTSSVTLRALPLLAVYTASKAAINAFTESL 154 Query: 61 RRELKGEGVHVLTVYPG 77 EL GV V V PG Sbjct: 155 ALELAPLGVQVRLVLPG 171 >gi|300311727|ref|YP_003775819.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 58.9 bits (141), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 34/111 (30%), Positives = 50/111 (45%) Query: 1 MIEVNLIAPILLTRDVMPDLRASGDGMVVNVASGIALVGVPFYATYAAAKAGISHFGEAL 60 + E N + + + V+P R GDG+++NV S L P A Y A+K I F +L Sbjct: 95 IFETNTFGVMAMCQAVIPQFRERGDGVIINVTSSATLAAFPLVAAYTASKTAIEGFTASL 154 Query: 61 RRELKGEGVHVLTVYPGATETPMMASSKAGPDLGFVRESAQAVAQAAVQGI 111 ELK GV V V PG + A++ G + E ++ A G Sbjct: 155 AHELKAVGVRVKLVEPGYGPSTSFATNGQQRMQGLITEPYESFAHEVFAGF 205 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 55.5 bits (132), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 31/81 (38%), Positives = 48/81 (59%), Gaps = 2/81 (2%) Query: 1 MIEVNLIAPILLTRDVMPDLRASGDGMVVNVASGIALVGVPFYATYAAAKAGISHFGEAL 60 + +V+L P LT+ ++P L A G G ++N++SG+A +P YA YA+ K G+ + Sbjct: 119 LFKVHLKGPFFLTQALLP-LLAQG-GRIINISSGLARFSLPGYAAYASMKGGVEVMTRYM 176 Query: 61 RRELKGEGVHVLTVYPGATET 81 +E G+ V TV PGA ET Sbjct: 177 AKEFGARGIVVNTVAPGAIET 197 >gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 55.5 bits (132), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 31/85 (36%), Positives = 44/85 (51%) Query: 1 MIEVNLIAPILLTRDVMPDLRASGDGMVVNVASGIALVGVPFYATYAAAKAGISHFGEAL 60 MI +N+ AP LT +P A G G V+N++S + + Y +KA + F ++L Sbjct: 114 MIALNVTAPTRLTYAAVPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSL 173 Query: 61 RRELKGEGVHVLTVYPGATETPMMA 85 EL +GV V V PGAT T A Sbjct: 174 HHELAAQGVQVQAVLPGATATDFWA 198 >gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 244 Score = 53.9 bits (128), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 30/84 (35%), Positives = 48/84 (57%) Query: 1 MIEVNLIAPILLTRDVMPDLRASGDGMVVNVASGIALVGVPFYATYAAAKAGISHFGEAL 60 +++VNL+ R V P +RA+ G +VN+AS G P + Y+AAKAG+ ++L Sbjct: 106 IVQVNLLGVFHACRSVTPAMRAARRGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSL 165 Query: 61 RRELKGEGVHVLTVYPGATETPMM 84 +EL GV V + P A +T ++ Sbjct: 166 GKELAESGVLVNAIAPAAVKTALL 189 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 477,086 Number of extensions: 18461 Number of successful extensions: 141 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 6 Length of query: 160 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 82 Effective length of database: 1,250,540 Effective search space: 102544280 Effective search space used: 102544280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)