BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0229.0007.p
         (373 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop...    65   7e-12
gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil...    62   5e-11
gi|300310022|ref|YP_003774114.1| glutathione S-transferase [Herb...    62   6e-11

>gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum
           seropedicae SmR1]
          Length = 300

 Score = 65.5 bits (158), Expect = 7e-12,   Method: Compositional matrix adjust.
 Identities = 40/129 (31%), Positives = 62/129 (48%), Gaps = 2/129 (1%)

Query: 238 FETVNGFQMHYVDEGDRAREVVLCLHGEPTWGYLYRSLITALCGDYRVVVPDHMGFGKSE 297
           F T+ G + H    GD     +  LHG       ++ ++ AL G + V+ PD  GFG +E
Sbjct: 14  FLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWHVIAPDWRGFGLTE 73

Query: 298 TPQDRSYWLQDHIDNLEKFVLQLDLND-VTLVMHDFGGPVGMGVTARHPGRIKRIISTNG 356
             Q  SYW  D++ +L+  +     ++ + L+ H  GG V        P RIKR+I+  G
Sbjct: 74  RSQADSYWFPDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGVRPQRIKRLINLEG 133

Query: 357 -PTPFGQPD 364
              P  QP+
Sbjct: 134 LGLPATQPE 142


>gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily
           protein [Herbaspirillum seropedicae SmR1]
          Length = 289

 Score = 62.4 bits (150), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 38/133 (28%), Positives = 62/133 (46%), Gaps = 1/133 (0%)

Query: 240 TVNGFQMHYVDEGDRAREVVLCLHGEPTWGYLYRSLITALCGDYRVVVPDHMGFGKSETP 299
           T++    H  + GD     +L LHG   +G  +  L   L   YR +  D  G G+S+  
Sbjct: 9   TLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWD 68

Query: 300 QDRSYWLQDHIDNLEKFVLQLDLNDVTLVMHDFGGPVGMGVTARHPGRIKR-IISTNGPT 358
              +Y+   ++ ++E  V +L+L    L+ H  GG   +   ARHP +++R II   GP 
Sbjct: 69  AGANYYTDAYVADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPG 128

Query: 359 PFGQPDGLERLLK 371
            F    G  R+ +
Sbjct: 129 AFENSPGATRIRR 141


>gi|300310022|ref|YP_003774114.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 204

 Score = 62.4 bits (150), Expect = 6e-11,   Method: Compositional matrix adjust.
 Identities = 53/184 (28%), Positives = 87/184 (47%), Gaps = 20/184 (10%)

Query: 1   MKIYDRPGFPNPARIRIVLAQKNLDSKVTFESIDLIGAEHKQAAFLAKNPSGVLPLLELD 60
           +K+      P   ++R+V+A+K +D +   E +       +Q+     NP G +P L +D
Sbjct: 2   LKLIGSNASPFVRKVRVVMAEKKIDYEFILEDVWSASTTIQQS-----NPLGKVPCLLMD 56

Query: 61  DDTLISESTAITEYLDNLDGNPVLTGRTPKEKAIIHMMQRRAESGLIDAVGIYFHHAT-- 118
           D   + +S  I EYLD L     L     +E+A I   +  A+ G++DA  +     T  
Sbjct: 57  DGGAMFDSRVIVEYLDTLTPVGKLLPPNGRERAEIKCWEALAD-GMLDAAVLVRLEKTLR 115

Query: 119 PGLGDDLQKYKSPEWAHRKEWGERERAKALQGMAYFDGILRDSSYVAGEQFSMADITVFA 178
           P       K +SPEW  R +W + E   +L+ MA  DG L +  +  G  +S+AD+ V  
Sbjct: 116 P------TKQQSPEWIKR-QWSKVE--ASLKAMA--DG-LGEKPFCVGNHYSLADVAVCC 163

Query: 179 GLAF 182
            L +
Sbjct: 164 ALGW 167


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.140    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,261,154
Number of extensions: 53813
Number of successful extensions: 139
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 139
Number of HSP's successfully gapped: 3
Length of query: 373
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 286
Effective length of database: 1,207,925
Effective search space: 345466550
Effective search space used: 345466550
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)