BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0228.0033.p (121 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 174 2e-45 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 54 3e-09 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 174 bits (440), Expect = 2e-45, Method: Composition-based stats. Identities = 78/111 (70%), Positives = 94/111 (84%) Query: 1 VAFDSLEGMMVTDADGKILRVNQAFTKITGYDVEDVIGKTPRLLRSDRHSPAFHRAIWES 60 VAFDS EGM++TDA+ KILRVN+AFT+ITGY E+++GK P LLRSDRH PAF + + ES Sbjct: 479 VAFDSREGMLITDANSKILRVNKAFTEITGYSAEEIVGKHPNLLRSDRHGPAFFQEMRES 538 Query: 61 VRHSGGWQGEIWNRRKNGQVYPEWLTISAVKSDDGVVTHYVGTHHDITERK 111 +R G WQGEIW+RRKNG+VYPEWLTISAVK+ D VTHYV THHDI++RK Sbjct: 539 IRRFGKWQGEIWDRRKNGEVYPEWLTISAVKNKDQQVTHYVSTHHDISDRK 589 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 54.3 bits (129), Expect = 3e-09, Method: Composition-based stats. Identities = 31/100 (31%), Positives = 47/100 (47%) Query: 13 DADGKILRVNQAFTKITGYDVEDVIGKTPRLLRSDRHSPAFHRAIWESVRHSGGWQGEIW 72 + D I VN AF +ITGY + +GK+ R L P +W ++R + Sbjct: 411 EPDYTIEYVNPAFARITGYSSTEAVGKSIRFLEGRDSDPEGFETVWIAMREQREHNVVLR 470 Query: 73 NRRKNGQVYPEWLTISAVKSDDGVVTHYVGTHHDITERKK 112 N K+G I+ V+ + G VTH+V +DIT K+ Sbjct: 471 NYHKDGSELWNDFYIAPVRDESGEVTHFVAALYDITSMKR 510 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 407,927 Number of extensions: 15955 Number of successful extensions: 64 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 68 Number of HSP's successfully gapped: 2 Length of query: 121 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 47 Effective length of database: 1,269,480 Effective search space: 59665560 Effective search space used: 59665560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)